Chenopodium ambrosioides volatile oil and application thereof in prevention and treatment of corn weevil and pholiota adiposa

A technology of Nepeta nepeta volatile oil and Tribulus chinensis, applied in application, insecticide, animal repellent, etc., can solve unsolved problems

Inactive Publication Date: 2020-12-15
SOUTHWEST FORESTRY UNIVERSITY
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0006] The above studies mainly focus on the application of the extract of Nepeta nepeta in agricultural pests. The extract contains alkaloids, glycosides, flavonoids, volatile oils, organic acids and other compounds, although the extract of Nepeta nepeta It can play a positive role in the control of agricultural pests. However, the above studies have not resolved which type of compound in the Nepeta extract is the effective inhibitory component, and whether the effective inhibitory component can improve its effect on agricultural pests. The problem of suppression effect

Method used

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  • Chenopodium ambrosioides volatile oil and application thereof in prevention and treatment of corn weevil and pholiota adiposa
  • Chenopodium ambrosioides volatile oil and application thereof in prevention and treatment of corn weevil and pholiota adiposa
  • Chenopodium ambrosioides volatile oil and application thereof in prevention and treatment of corn weevil and pholiota adiposa

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0019] Example 1: Extraction and determination of the volatile oil of Schizonepeta yunnanensis

[0020] The above-ground part of Nepeta nepeta in this example is collected from Yiliang County, Kunming City, Yunnan Province, longitude: 103°3′43″, latitude: 24°58′27″, altitude: 1742m, through the Dendrology Teaching and Research Office of Forestry College, Southwest Forestry University Dr. Liu Weiyi identified it as Dysphania ambrosioides of Chenopodiaceae, and it was dried in the shade for later use.

[0021] The extraction process of the volatile oil of Nepeta yunnanensis is as follows: cut the fresh Nepeta yunnanensis sample into pieces, put it into a 1000mL round bottom flask, add appropriate amount of water, use a volatile oil extractor, extract by steam distillation for 5 hours, and obtain a light yellow oil The material was freeze-dried, formulated into solutions of different concentrations, and stored at -4°C for future use.

[0022] The determination procedure of Nepet...

Embodiment 2

[0031] Example 2: Application of the volatile oil of Nepeta yunnanensis in the prevention and control of corn weevil and red beetle by fumigation

[0032] (1) Experiments of corn elephant and red-spotted corn beetle

[0033] The source of corn weevils for testing is provided by the Natural Fun Insect and Plant Hall of Qushou, Xichuan County, Nanyang City, Henan Province, in a constant temperature incubator (temperature 27°C ± 2°C, relative humidity 75% ± 5%, photoperiod L: D = 16h: 8h ) were fed with wheat, placed in an insect culture room for 7 days, and then the adults were screened out; after a new generation of adults emerged in large numbers for about a week, the adults were screened out as test insects.

[0034] For the test, the source of the red pseudograss is provided by the Natural Interesting Insect Plant Hall of Xichuan County, Nanyang City, Henan Province, cultivated in an indoor artificial climate incubator (temperature 27 ° C ± 2 ° C, relative humidity 75% ± 5%,...

Embodiment 3

[0046] Example 3: Application of the volatile oil of Nepeta yunnanensis in the prevention and control of corn weevil and red beetle through contact killing

[0047] (1) Experiments of corn elephant and red-spotted corn beetle

[0048] With embodiment 2.

[0049] (2) Determination of contact activity

[0050] The contact killing activity of the volatile oil of Nepeta nepeta on the corn weevil and the red beetle was determined by the micro-drop method. Determine the test concentration range through preliminary experiments. The contact concentration of nepeta volatile oil on corn weevil is: 112.5mg / mL, 56.25mg / mL, 28.125mg / mL, 14.06mg / mL, 7.03mg / mL. The contact concentrations for Trichosmus chinensis are: 50mg / mL, 10mg / mL, 2mg / mL, 0.4mg / mL, 0.08mg / mL. Use a pipette to take 0.5 μL of solutions of different concentrations and drop them on the pronotum of the corn elephant and the red corn beetle. Each concentration treats 10 test insects, repeats 5 times, and transfers them to ...

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Abstract

The invention discloses chenopodium ambrosioides volatile oil, and the oil is prepared from the following compounds in percentage by weight: 36.136 percent of (+)-4-carene, 23.897 percent of 2-carene,10.571 percent of o-cymene, 9.047 percent of ascaritin epoxide, 4.882 percent of ascaritin and the balance of trace compounds. Meanwhile, the invention further discloses the chenopodium ambrosioidesvolatile oil which can be used for preventing and treating zeamazus maydis and pholidota rufida through fumigation, contact killing and repelling. The use concentrations of the chenopodium ambrosioides volatile oil for preventing and treating the corn weevil and the pholidota erythrorhizon through fumigation are 1.35 mg / L and 2.83 mg / L respectively. The use dosages of the chenopodium ambrosioidesvolatile oil for preventing and treating the corn weevil and the pholidota erythrorhizon through contact toxicity are 16.8 [mu]g / head and 29.35 [mu]g / head respectively. The use concentrations of the chenopodium ambrosioides volatile oil for preventing and treating the corn weevil and the pholidota erythrorhizon through repelling are 0.094 to 58.975 [mu]g / cm <2> and 58.975 [mu]g / cm <2> respectively. The chenopodium ambrosioides volatile oil has a good prevention and treatment effect on zeastrum maydis and pholiota adiposa, and can be used for prevention and treatment in a fumigation, contact killing and repelling manner.

Description

technical field [0001] The invention relates to the field of application of botanical pesticides in storage pests, in particular to the application of the volatile oil of nepeta yunnanensis in preventing and treating corn weevil and red scorpion beetle. Background technique [0002] Corn weevil (Sitophilus zeamais Motschulsky) belongs to Coleoptera (Coleoptera) insects of the family Curculionidae. It is distributed all over the world and endangers stored goods such as rice, wheat, sorghum, corn, oilseeds, Chinese medicinal materials, and grain products. Stored grain pests are not only the main cause of the decline in the quantity and quality of stored grain, but also an important medium for grain mildew and the spread of pathogenic microorganisms, which seriously endanger the physical and mental health of consumers. [0003] Tribolium castaneum Herbst (Tribolium castaneum Herbst) belongs to Coleoptera (Coleoptera) and is an insect of the family Tenebrionidae. It is also an i...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): A01N27/00A01N43/90A01N65/08A01P7/04
CPCA01N27/00A01N43/90A01N65/08
Inventor 梁倩
Owner SOUTHWEST FORESTRY UNIVERSITY
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