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Method for detecting transgenic components of corn by utilizing quadruple real-time fluorescent PCR

A technology of transgenic ingredients and real-time fluorescence, applied in biochemical equipment and methods, recombinant DNA technology, microbial measurement/inspection, etc., can solve problems that cannot meet the needs of genetically modified food supervision

Pending Publication Date: 2020-12-25
ZHEJIANG ECONOMIC & TRADE POLYTECHNIC
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  • Abstract
  • Description
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  • Application Information

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Problems solved by technology

At present, with the introduction of exogenous gene types of agricultural products and the approval of transgenic production and application of more and more crops, the detection of a single item can no longer meet the needs of genetically modified food supervision, and there is an urgent need to develop high-throughput rapid genetically modified detection technology

Method used

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  • Method for detecting transgenic components of corn by utilizing quadruple real-time fluorescent PCR
  • Method for detecting transgenic components of corn by utilizing quadruple real-time fluorescent PCR
  • Method for detecting transgenic components of corn by utilizing quadruple real-time fluorescent PCR

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Embodiment Construction

[0063] 1 Materials and methods

[0064] 1.1 Sample collection

[0065] The maize positive samples were mainly "MON810", "MON863", "NK603", "MON88017", "Bt176", "Bt11", "FAPAS GeMSU 56A", "FAPAS GeMSU 56B", "ACAS-T067-J273". The maize negative samples were "ZY1711", "Meiyu No. 7", "Cainuo No. 1", "Jingnian No. 1" and "Tiannuo No. 182". The corn deep-processing products are the circulating products purchased by the laboratory in the market in 2019, with a total of 50 batches.

[0066] 1.2 Genomic DNA extraction

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Abstract

The invention discloses a method for detecting transgenic components of corn by utilizing quadruple real-time fluorescent PCR, and primers and probes used in the method. The method disclosed by the invention comprises the following steps of designing the primers and the probes required by quadruple real-time fluorescent PCR amplification; extracting genome DNA in the corn to be detected; carryingout quadruple real-time fluorescent PCR amplification; and judging whether the corn to be detected is transgenic corn or not according to a PCR amplification result. The invention provides the effective method for rapidly detecting whether the corn and a deep processing product thereof are the transgenic corn or not.

Description

technical field [0001] The invention relates to transgenic corn and its deep-processed products, in particular to a quadruple real-time fluorescent PCR detection method applicable to whether the corn and its deep-processed products contain genetically modified ingredients (that is, whether it is genetically modified corn), and belongs to the field of food safety transgenic detection. Background technique [0002] Corn is the second largest commercial food crop in the world. The global planting area is about 60.6 million hectares, accounting for 45.1% of the global transgenic planting area. The transgenic penetration rate has reached 33%, making it the second largest crop in planting area. At present, there are 146 lines of genetically modified corn in the world, mainly including genetically modified traits such as herbicide resistance, insect resistance, quality improvement, drought tolerance, insect resistance + herbicide tolerance, quality and herbicide tolerance. As a maj...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): C12Q1/6895C12Q1/6851C12N15/11
CPCC12Q1/6895C12Q1/6851C12Q2600/13C12Q2600/16C12Q2600/166C12Q2531/113C12Q2537/143C12Q2563/107C12Q2545/101
Inventor 王凤军叶素丹黄涵年
Owner ZHEJIANG ECONOMIC & TRADE POLYTECHNIC