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CpG oligodeoxynucleotide, preparation, and application thereof

A technology of deoxynucleotides and nucleotides, which is applied in the field of compound adjuvants and preparations, can solve the problems of weak immunostimulatory activity, and achieve the effects of low toxic and side effects, reduced hydrolysis, and simple preparation methods

Pending Publication Date: 2021-01-01
LANZHOU INST OF VETERINARY SCI CHINESE ACAD OF AGRI SCI
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  • Abstract
  • Description
  • Claims
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AI Technical Summary

Problems solved by technology

Studies have found that the immunostimulatory activity of a simple CpG motif containing 6 deoxynucleotides is extremely weak. To make it exert the best immunostimulatory effect, the length of CpG must be increased, generally 15-30 bases

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  • CpG oligodeoxynucleotide, preparation, and application thereof
  • CpG oligodeoxynucleotide, preparation, and application thereof
  • CpG oligodeoxynucleotide, preparation, and application thereof

Examples

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Embodiment Construction

[0028] The present invention is explained in detail below in conjunction with examples. The experimental methods used in the following examples are conventional methods unless otherwise specified; the materials and reagents used in the following examples can be obtained from commercial sources unless otherwise specified.

[0029] 1. Design and screening of CpG ODN molecules

[0030] (1) Screening of CpG ODN molecules

[0031] Through the screening and verification of the structure, function and function of four CpG ODN sequences (see Table 2), a CpG ODN sequence CpG ODN 46-mod with a strong immune enhancing effect was screened out, and its nucleotide sequence is SEQ ID No. 1, and all nucleotides are all sulfur-modified, referred to as CpG ODN 46.

[0032] The CpG ODN nucleotide backbone in Table 2 is that all nucleotides are not thio-modified (name suffix is ​​-non) and all nucleotides are fully thio-modified (name suffix is ​​-mod). The non-CpG control does not contain CpG...

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Abstract

The invention discloses preparation and application of a composite adjuvant containing CpG oligodeoxynucleotide. The invention provides the CpG oligodeoxynucleotide with a sequence shown as SEQ ID No.1, and all nucleotides are subjected to sulfo-modification. The CpG oligodeoxynucleotide has the capability of enhancing B cell proliferation and Th I type immune response. The sulfo-modification prolongs the residence time of the CpG oligodeoxynucleotide in vivo, and reduces the hydrolysis effect of various hydrolases on the CpG oligodeoxynucleotide. The preparation method is simple, the qualityis easy to control, large-scale production is easy, safety is good, and toxic and side effects are low. The invention provides the preparation method of the composite adjuvant containing the CpG oligodeoxynucleotide, and the composite adjuvant is prepared by mixing and emulsifying the CpG oligodeoxynucleotide and an oil emulsion ISA 206 according to a certain proportion, so that the process is simple, and the performance is stable. The composite adjuvant containing the CpG oligodeoxynucleotide can promote the advanced production of antibodies and has a strong immunopotentiation effect.

Description

technical field [0001] The invention relates to a CpG oligodeoxynucleotide, a compound adjuvant containing the CpG oligodeoxynucleotide, a preparation method and use thereof. Background technique [0002] In the process of vaccine development, people realized that the successful preparation of vaccines must study the compatibility of adjuvants and vaccine components so that the two can form a stable, safe and immunogenic vaccine complex. An adjuvant is a non-specific immunopotentiator, which can enhance the body's immune response to the antigen or change the type of immune response together with the antigen or after pre-injection into the body. Therefore, as a non-specific immune enhancer, adjuvants play a vital role in inducing an effective immune response after vaccination. Commonly used adjuvants include: mineral adjuvants such as aluminum salt adjuvant, oil emulsion adjuvant such as ISA206, cytokine adjuvant, microbial component adjuvant such as CpG-ODN, LPS (CpG-ODN as...

Claims

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Application Information

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IPC IPC(8): C12N15/117A61K39/39A61K39/135A61P31/14A61P37/04
CPCC12N15/117A61K39/39A61K39/12A61P31/14A61P37/04C12N2310/17A61K2039/55561A61K2039/5252A61K2039/552C12N2770/32134A61K2039/55566
Inventor 景志忠房永祥赵波贾怀杰陈国华何小兵沈岩尔李政厅杨帆刘娟秦娟
Owner LANZHOU INST OF VETERINARY SCI CHINESE ACAD OF AGRI SCI
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