PROS1 gene molecular marker related to chicken carcass traits and application

A technology of carcass traits and molecular markers, applied in the field of molecular biotechnology and molecular markers, can solve the problems of low meat production, poor reproductive performance, low feed conversion rate, etc., to achieve increased meat production, improved anticoagulant effect, The effect of improving the efficiency of breeding

Active Publication Date: 2021-01-05
佛山市南海种禽有限公司
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  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

Yellow-feathered broiler chickens, especially local chicken breeds, generally have problems such as low feed conversion rate, low meat production and poor reproductive performance, which have great requirements on breeding costs and feed consumption

Method used

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  • PROS1 gene molecular marker related to chicken carcass traits and application
  • PROS1 gene molecular marker related to chicken carcass traits and application
  • PROS1 gene molecular marker related to chicken carcass traits and application

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Experimental program
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Embodiment Construction

[0030] 1. Animal samples

[0031] 320 80-day-old Guangdong jute broilers were selected, and 1 mL of subcutaneous venous blood was collected and stored at -30°C for DNA extraction. Carcass traits such as body weight, shin length, eviscerated weight, half eviscerated weight, and breast muscle weight were measured.

[0032] 2. Main reagents

[0033] Blood Sample DNA Extraction Kit (Brand: OMEGA; Item No.: D3392; Guangzhou Feiyang Bioengineering Co., Ltd.), T3Super PCR Mix (Brand: Qingke; Item No.: TSE030; Beijing Qingke Xinye Biotechnology Co., Ltd.), DNA marker (Brand : Quanshijin; Item No.: BM101-01; Beijing Quanshijin Biotechnology Co., Ltd.).

[0034] 4 Primer Design for PCR Amplification of 5' Flanking Region Sequence of Chicken PROS1 Gene

[0035] According to the 5' flanking region sequence of the chicken PROS1 gene (NC_006088.5:91130895-91132555) published by NCBI (National Center for Biotechnology Information Searchdatabase), primers were designed using Primer Premier...

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Abstract

The invention discloses a PROS1 gene molecular marker related to chicken carcass traits and application. The molecular marker is located in a 5' flanking region of a PROS1 gene. Therefore, the prejudice in the prior art is overcome; in the prior art, PROS1 protein is considered not to have enzymatic activity, and the function of the PROS1 protein is mainly exerted by being used as an auxiliary factor to assist activated protein C, so that the anticoagulation effect of the activated protein C is improved; and the molecular marker in the 5' flanking region of the PROS1 gene can influence the chicken carcass traits and is applied to breeding of the chicken carcass traits, so that the breeding efficiency is improved and the meat yield of chickens is improved.

Description

technical field [0001] The invention relates to the technical fields of molecular biotechnology and molecular markers, in particular to a PROS1 gene molecular marker related to chicken carcass traits and its application. Background technique [0002] Chicken is the second largest meat consumer product in my country, derived from white-feather broilers and yellow-feather broilers. In the broiler chicken breeding industry, the weight and meat yield of broiler chickens are positively correlated with the market economic benefits, which directly affects the development of the broiler chicken breeding industry. Compared with white-feather broilers, yellow-feather broilers have better meat quality and better flavor, but there are also major disadvantages. Yellow-feathered broiler chickens, especially local chicken breeds, generally have problems such as low feed conversion rate, low meat production and poor reproductive performance, which have great requirements on breeding costs ...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): C12Q1/6888A01K67/02
CPCA01K67/02C12Q1/6888C12Q2600/124C12Q2600/156C12Q2600/172
Inventor 聂庆华郭利金黄卫玲黎镇晖徐海平郑茗
Owner 佛山市南海种禽有限公司
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