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HPLC content determination method for naringenin in pollen pini

A method of determination, technology of naringenin, applied in the direction of measuring devices, instruments, scientific instruments, etc., to achieve the effect of simple operation, high content and strong specificity

Pending Publication Date: 2021-01-26
SHANGHAI HUANGHAI PHARMA
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

Control and characterization of undetermined chemical constituents of pine pollen

Method used

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  • HPLC content determination method for naringenin in pollen pini
  • HPLC content determination method for naringenin in pollen pini
  • HPLC content determination method for naringenin in pollen pini

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Experimental program
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Effect test

Embodiment 1

[0021] 1 Materials and methods

[0022] 1.1 Experimental materials

[0023] Pine pollen samples were provided by Shanghai Huanghai Pharmaceutical Co., Ltd. (Table 6). Naringenin reference substance, batch number: 3597, purity: 98%, was purchased from Shanghai Dande Standard Technology Service Co., Ltd.; HPLC grade methanol and 96% phosphoric acid were purchased from Shanghai Anpu Technology Co., Ltd.

[0024] 1.2 Experimental Instruments

[0025] Agilent1260 high-performance liquid chromatography (Agilent); HWS24 electric heating constant temperature water bath (Shanghai-Heng Technology Co., Ltd.); XP-105 electronic balance (Mettler-Toledo); Diamonsil C 18 Chromatographic column (4.6mm×150mm, 5μm, Shanghai Dicoma Analytical Technology Co., Ltd.).

[0026] 1.3 Determination method of naringenin content

[0027] 1.3.1 Chromatographic conditions

[0028] Mobile phase: methanol-0.05% phosphoric acid solution (45:55); column temperature: 30°C; detection wavelength: 288nm; flow...

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Abstract

The invention relates to an HPLC content determination method for naringenin in pollen pini, establishes a content determination method for naringenin components in pollen pini, performs content determination on naringenin of pollen pini from multiple producing areas, and has the characteristics of simplicity and convenience in operation, quickness, strong specificity and good repeatability. The naringenin is stable and high in content in the pollen pini, so that the naringenin can be used as an index component for quality control of pollen pini medicinal materials, has important significancefor research of the pollen pini, is beneficial to subsequent research of the medicinal materials, and is beneficial to quality control and characterization of traditional Chinese medicines such as body resistance strengthening and blood stasis removing tablets / capsules and other pollen pini-containing health care products.

Description

technical field [0001] The invention belongs to the field of pine pollen determination, in particular to a method for determining the content of naringenin in pine pollen by HPLC. Background technique [0002] Pine pollen is the dry pollen of Pinus massoniana Lamb., Pinus pine (Pinustabulieformis Carr.) or several plants of the same genus. Pine pollen, also known as pine yellow and pine flower, is one of the traditional medicinal and edible materials in my country. It has the effects of astringent and hemostasis, and can be used for traumatic bleeding, skin erosion, eczema, and yellow water sores. Pine pollen is one of the raw materials of traditional Chinese medicine Fuzheng Huayu tablets / capsules (Z20020047 / Z20050573), as well as one of the raw materials for health products such as pine pollen ganoderma tablets and pine pollen dandelion granules. Pine pollen is usually picked when the flowers just bloom in spring, the flower spikes are dried, the pollen is collected, and ...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): G01N30/02
CPCG01N30/02
Inventor 潘一峰张玉杰李荣胜林超颖
Owner SHANGHAI HUANGHAI PHARMA
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