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Aspergillus strain GW13 and application thereof

A technology of Aspergillus and bacterial strains, which is applied in the field of Aspergillus versicolor toxin production, and can solve problems such as the inability to achieve domestic production

Inactive Publication Date: 2021-01-29
BEIJING TECHNOLOGY AND BUSINESS UNIVERSITY
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

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Problems solved by technology

[0004] Mycotoxins are a must-test item in the grain production process. Commonly used mycotoxin detection methods include high-efficiency liquid chromatography, gas chromatography, enzyme-linked immunosorbent assay, and some chromatography-mass spectrometry techniques. However, most of these tests currently rely on imported reference materials As the reference substance, domestic production cannot be realized

Method used

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  • Aspergillus strain GW13 and application thereof
  • Aspergillus strain GW13 and application thereof
  • Aspergillus strain GW13 and application thereof

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Effect test

Embodiment 1

[0014]1. Collection of strain GW13

[0015]In September 2019, a strain was isolated from corn collected in Xianyang City, Shaanxi Province and named as strain GW13.

[0016]2. Identification of strain GW13

[0017]The BT partial sequence of strain GW13 is shown in sequence 1 (SEQ ID NO. 1) in the sequence table, which has the highest sequence similarity with GENBANK ACCESSIONNO.JN854007.1, with a similarity of 99.5%.

[0018]According to the above identification results, the strain GW13 belongs to Aspergillus sp.

[0019]3. Preservation of strain GW13

[0020]Strain GW13, belonging to Aspergillus sp (Aspergillus sp), has beenJuly 16, 2020 Deposited in the General Microbiology Center of the China Microbial Culture Collection Management Committee (referred to as CGMCC, address: No. 3, No. 1, Beichen West Road, Chaoyang District, Beijing), and the preservation center registration number is CGMCCNo.19931 .

[0021]4. Preparation of variegated aspergillus toxin by strain GW13

[0022]1) Take the strain GW13 sto...

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Abstract

The invention discloses an aspergillus sp. Strain GW13 CGMCC No.19931 and application of the aspergillus sp. Strain GW13 in preparation of sterigmatocystin. The strain is preserved in China General Microbiological Culture Collection Center (CGMCC) on July 16th, 2020 (CGMCC for short), the address is No.3, Yard 1, West Beichen Road, Chaoyang District, Beijing), and the registration number of the preservation center is CGMCC No.19931. The industrial technical problem of preparing the high-purity sterigmatocystin standard substance is effectively solved.

Description

Technical field[0001]The present invention relates to the technical field of Aspergillus producing versicolor aspergillus toxin.Background technique[0002]Versicolor aspergillus toxin (ST) is mainly produced by Aspergillus versicolor, Aspergillus pyogenes, Aspergillus versicolor, Aspergillus clawus, Chaetomium and Aspergillus flavus. These molds are widely found in wheat, barley, corn, peanuts, soybeans and other grains, and the most toxic product is Aspergillus versicolor.[0003]Versicolor is one of the carcinogenic mycotoxins. Although its toxicity is weaker than that of aflatoxin, the main toxin-producing bacteria Aspergillus versicolor and Aspergillus nidulans are widely distributed in food and feed, and the proportion of toxin production is high, so the harm to humans, livestock and poultry cannot be ignored.[0004]Mycotoxins are a must-check item in the food production process. Commonly used mycotoxins detection methods include high performance liquid chromatography, gas chromato...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): C12N1/14C12P17/18C12R1/66
CPCC12P17/181C12R2001/66C12N1/145
Inventor 宋福行李二伟徐秀丽王龙杨娜李红花张楷
Owner BEIJING TECHNOLOGY AND BUSINESS UNIVERSITY
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