Human motor neuron gene copy number relative quantitative detection method and kit
A technology of human motor neuron and gene copy number, which is applied in the determination/inspection of microorganisms, biochemical equipment and methods, DNA/RNA fragments, etc. Quantitative result deviation and other problems, to achieve high accuracy, reduce birth rate, and good repeatability
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Embodiment 1
[0062] Embodiment 1, the composition of kit of the present invention
[0063] 1. Common primers for specific amplification of exon 7 of SMN1 and SMN2 genes:
[0064] Upstream primer SMN-EX7-F: 5'-CTTGTGAAACAAAATGCTTTTTA-3' (SEQ ID No.1);
[0065] Downstream primer SMN-EX7-R: 5'-GAATGTGAGCACCTTCCTTCTTT-3' (SEQ ID No. 2).
[0066] 2. Common primers for specific amplification of exon 8 of SMN1 and SMN2 genes:
[0067] Upstream primer SMN-EX8-F: 5'-GTTATGTAATAACCAAATGCAA-3' (SEQ ID No.3);
[0068] Downstream primer SMN-EX8-R: 5'-TTCTCAACTGCCTCACCACCG-3' (SEQ ID No. 4).
[0069] 3. PCR amplification primers of internal reference gene RPP30:
[0070] Upstream primer RPP30-F: 5'-TTTGGACCTGCGAGCG-3' (SEQ ID No.9);
[0071] Downstream primer RPP30-R: 5'-GAGCGGCTGTCTCCAC-3' (SEQ ID No. 10).
[0072] 4. Probe for detecting exon 7 of SMN1 gene:
[0073] SMN1-EX7-P: FAM-AGG+GTTT+CAGAC-BHQ1 (SEQ ID No. 5).
[0074] 5. Probe for detecting exon 7 of SMN2 gene:
[0075] SMN2-EX7-P: VI...
Embodiment 2
[0085] Embodiment 2, genomic DNA preparation
[0086]1ml of human peripheral venous blood was collected according to medical routine, and EDTA was added for anticoagulation. Use QIAGEN’s Human Peripheral Blood Genome Extraction Kit to extract genomic DNA. The elution volume is 100 μL. Quantify it with a UV spectrometer. The ratio of OD260nm / OD280nm should be between 1.8 and 2.0. Use deionized water to dilute the genomic DNA. Concentration to 10-20ng / μL.
Embodiment 3
[0087] Embodiment 3, gradient dilution of reference substance
[0088] Preparation of reference substance: Dilute the reference substance with TE to 0.5pg / μL, and then serially dilute to establish three concentration gradients of 1:5:25 (stock solution, 5-fold dilution and 25-fold dilution) for use.
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