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Multi-scattering system adaptive fluorescence detection system and detection method

A fluorescent detection and self-adaptive technology, which is applied in the direction of fluorescence/phosphorescence, measuring devices, and material analysis through optical means, can solve the problems of reducing the accuracy of detection results, errors in the intensity of fluorescent signals, and failure to cope, and achieve the elimination of fluorescent signals Intensity difference, effect of eliminating quantitative detection error

Active Publication Date: 2022-07-19
SUZHOU INST OF BIOMEDICAL ENG & TECH CHINESE ACADEMY OF SCI
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  • Abstract
  • Description
  • Claims
  • Application Information

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Problems solved by technology

[0004] The traditional fluorescence detection optical system only detects the fluorescent signal entering the detection optical path, and cannot cope with the error in the intensity of the fluorescent signal caused by the difference in the scattering system in the process of fluorescence immunoassay, thus reducing the quantitative accuracy of the detection results

Method used

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  • Multi-scattering system adaptive fluorescence detection system and detection method
  • Multi-scattering system adaptive fluorescence detection system and detection method
  • Multi-scattering system adaptive fluorescence detection system and detection method

Examples

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Embodiment 1

[0032] like figure 1 As shown, a multi-scattering system adaptive fluorescence detection system in this embodiment includes: a fluorescence collection lens 101, a main dichroic mirror 102, an excitation light path 103, a secondary dichroic mirror 104, a first converging lens 105, a first photoelectric conversion detector 106, a bandpass filter 107, a second converging lens 108, and a second photoelectric conversion detector 109;

[0033] The parallel excitation light emitted by the excitation light path 103 is incident on the main dichroic mirror 102 and then reflected on the fluorescence collection lens 101, and then converged by the fluorescence collection lens 101 and then irradiated into the sample;

[0034] The fluorescence generated by the excitation of the sample is collimated by the fluorescence collection lens 101 and then transmitted through the main dichroic mirror 102 and the secondary dichroic mirror 104 in turn, and then filtered by the bandpass filter 107 and th...

Embodiment 2

[0050] This embodiment provides a multi-scattering system adaptive fluorescence detection method, which uses the multi-scattering system adaptive fluorescence detection system of Embodiment 1 to perform fluorescence detection on a sample, and then uses the target fluorescence energy obtained by the second photoelectric conversion detector 109 The ratio of the intensity to the excitation light energy intensity obtained by the first photoelectric conversion detector 106 is used to calculate the concentration of the sample to be tested.

[0051] Since the macromolecular clusters in the sample have the same scattering effect on the incident excitation light and the emitted fluorescence, when the scattering system of the sample changes, the scattered excitation light and The ratio of fluorescence varies quite a bit; since the ratio of reflection, transmission, and absorption of each optical device in the same optical system to different wavelengths is fixed, when the sample scatteri...

Embodiment 3

[0061] The comparison between the detection system of the present invention and the conventional fluorescence detection system, wherein, compared with the present invention, the conventional fluorescence detection system lacks the secondary dichroic mirror 104 and the first photoelectric conversion detector 106, and the second photoelectric conversion detector 109 detects only the fluorescent signal entering the detection optical path.

[0062] The detection system and the conventional fluorescence detection system of Example 2 of the present invention are used to detect the same sample. The specific measurement method and process are as follows: First, a test sample is divided into 7 equal parts, and the numbers are respectively 1-7, that is, the numbering There are 7 sub-samples from 1 to 7. Since the 7 sub-samples are all from the same test sample, their material composition is the same; then the 7 sub-samples are pre-treated according to the same method steps. The conventi...

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Abstract

The invention discloses a multi-scattering system adaptive fluorescence detection system and a detection method. The system comprises: a fluorescence collection lens, a main dichroic mirror, an excitation light path, a secondary dichroic mirror, a first converging lens, a first photoelectric a conversion detector, a bandpass filter, a second converging lens, and a second photoelectric conversion detector. The invention can synchronously detect the excitation light signal scattered by the sample and the fluorescence signal emitted, and use the scattered excitation light as a reference, so that the target fluorescence energy measurement data can be automatically synchronously corrected according to the reference light intensity, and the suspension in different samples can be excluded. The fluorescence signal intensity difference caused by uneven particle scattering can eliminate the quantitative detection error caused by the change of scattering system, can adapt to a variety of different scattering systems, and resist the interference of uneven sample scattering. It is suitable for the size and surface morphology of suspended particles. , Fluorescence signal detection of suspension samples of different scattering systems with different uniformity.

Description

technical field [0001] The invention relates to the technical field of optical equipment, in particular to a multi-scattering system adaptive fluorescence detection system and a detection method. Background technique [0002] Fluorescence immunoassay is a method that combines the specificity of the immunological reaction with the sensitivity of the fluorescence technology. It is mostly used to detect biologically active compounds such as proteins, hormones, drugs, and microorganisms. The macromolecular clusters in the sample are easy to agglomerate and form a suspension. state, which scatters the incident excitation light and the emitted fluorescence. [0003] The length of the sample placement time and the different batches make the size, surface morphology and uniformity of the suspended particles different (ie the difference in the scattering system). On the one hand, it affects the scattering and light energy absorption of the incident excitation light by the sample. It...

Claims

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Application Information

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Patent Type & Authority Patents(China)
IPC IPC(8): G01N21/64
CPCG01N21/645G01N2021/6482G01N2021/6491G01N2021/6471G01N2021/6463
Inventor 赵振英王弼陡孙海旋
Owner SUZHOU INST OF BIOMEDICAL ENG & TECH CHINESE ACADEMY OF SCI
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