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Multi-scattering system self-adaptive fluorescence detection system and detection method

A fluorescence detection and self-adaptive technology, applied in fluorescence/phosphorescence, measurement devices, material analysis through optical means, etc., can solve the problems of reducing the accuracy of detection results, the error of fluorescence signal intensity, and the inability to deal with it, so as to eliminate the fluorescence signal Intensity difference, effect of eliminating quantitative detection error

Active Publication Date: 2021-01-29
SUZHOU INST OF BIOMEDICAL ENG & TECH CHINESE ACADEMY OF SCI
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  • Abstract
  • Description
  • Claims
  • Application Information

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Problems solved by technology

[0004] The traditional fluorescence detection optical system only detects the fluorescent signal entering the detection optical path, and cannot cope with the error in the intensity of the fluorescent signal caused by the difference in the scattering system in the process of fluorescence immunoassay, thus reducing the quantitative accuracy of the detection results

Method used

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  • Multi-scattering system self-adaptive fluorescence detection system and detection method
  • Multi-scattering system self-adaptive fluorescence detection system and detection method
  • Multi-scattering system self-adaptive fluorescence detection system and detection method

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Embodiment 1

[0032] Such as figure 1 As shown, a multi-scattering system adaptive fluorescence detection system in this embodiment includes: a fluorescence collecting lens 101, a primary dichroic mirror 102, an excitation light path 103, a secondary dichroic mirror 104, a first converging lens 105, A first photoelectric conversion detector 106, a bandpass filter 107, a second converging lens 108, and a second photoelectric conversion detector 109;

[0033] The excitation light in a parallel state emitted by the excitation light optical path 103 is incident on the main dichroic mirror 102 and then reflected on the fluorescence collection lens 101, and then irradiated into the sample after being converged by the fluorescence collection lens 101;

[0034] The fluorescence generated by the excitation of the sample is collimated by the fluorescence collecting lens 101, then transmits the primary dichroic mirror 102 and the secondary dichroic mirror 104 sequentially, and then is filtered by the ...

Embodiment 2

[0050] This embodiment provides a multi-scattering system adaptive fluorescence detection method, which uses the multi-scattering system adaptive fluorescence detection system in Embodiment 1 to perform fluorescence detection on the sample, and then uses the target fluorescence energy obtained by the second photoelectric conversion detector 109 The concentration of the sample to be tested is calculated by the ratio of the intensity to the energy intensity of the excitation light obtained by the first photoelectric conversion detector 106.

[0051] Since the macromolecular clusters in the sample have the same scattering effect on the incident excitation light and the emitted fluorescence, when the sample scattering system changes, the scattered excitation light and The proportion of fluorescence changes quite; since the proportions of reflection, transmission and absorption of each optical device in the same optical system to different wavelengths are fixed, when the sample scat...

Embodiment 3

[0061] Comparison between the detection system of the present invention and the conventional fluorescence detection system, wherein, compared with the present invention, the conventional fluorescence detection system lacks the secondary dichroic mirror 104 and the first photoelectric conversion detector 106, and the second photoelectric conversion detector 109 only detects the fluorescent signal entering the detection light path.

[0062] The detection system of Example 2 of the present invention and the conventional fluorescence detection system are used to detect the same sample. The specific measurement method and process are as follows: first, a test sample is divided into 7 equal parts, respectively numbered 1-7, that is, numbered For the 7 sub-samples of 1-7, since the 7 sub-samples all come from the same test sample, their material composition is the same; then the 7 sub-samples are subjected to sample pretreatment according to the same method steps, after the treatment ...

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Abstract

The invention discloses a multi-scattering system self-adaptive fluorescence detection system and a detection method. The system comprises a fluorescence collection lens, a main dichroscope, an exciting light path, an auxiliary dichroscope, a first converging lens, a first photoelectric conversion detector, a band-pass filter, a second converging lens and a second photoelectric conversion detector. According to the invention, excitation light signals scattered by a sample and emitted fluorescence signals can be synchronously detected, and scattered excitation light is used as a reference so that target fluorescence energy measurement data can be automatically and synchronously corrected according to the reference light intensity, and a fluorescence signal intensity difference caused by uneven scattering of suspended particles in different samples can be eliminated; and therefore, quantitative detection errors caused by changes of the scattering system can be eliminated, the system canadapt to various different scattering systems, interference of uneven scattering of the sample is resisted, and the system is suitable for fluorescence signal detection of turbid liquid samples of different scattering systems with differences in size, surface morphology and uniformity of suspended particles.

Description

technical field [0001] The invention relates to the technical field of optical equipment, in particular to a multi-scattering system adaptive fluorescence detection system and a detection method. Background technique [0002] Fluorescence immunoassay is a method that combines the specificity of immunological reactions and the sensitivity of fluorescence techniques. It is mostly used to detect biologically active compounds such as proteins, hormones, drugs, and microorganisms. Supermacromolecular clusters in samples are easy to condense to form suspensions. state, which has a scattering effect on the incident excitation light and the emitted fluorescence. [0003] The length of time the sample is placed and the difference in batches lead to differences in the size, surface morphology, and uniformity of the suspended particles (that is, the difference in the scattering system), which affects the scattering of the incident excitation light and light energy absorption by the sam...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): G01N21/64
CPCG01N21/645G01N2021/6482G01N2021/6491G01N2021/6471G01N2021/6463
Inventor 赵振英王弼陡孙海旋
Owner SUZHOU INST OF BIOMEDICAL ENG & TECH CHINESE ACADEMY OF SCI
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