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Application of biomarker RP11-54A9.1 in prediction of oral squamous cell carcinoma and treatment of oral squamous cell carcinoma

A biomarker, oral squamous cell carcinoma technology, applied in the determination/inspection of microorganisms, biochemical equipment and methods, medical preparations containing active ingredients, etc. Problems with chewing and swallowing

Inactive Publication Date: 2021-02-05
XIANGYA STOMATOLOGICAL HOSPITAL CENT SOUTH UNIV
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

Among them, CT and MRI can only detect the actual existence of the quality, but cannot verify whether the quality is benign or malignant. Due to the characteristics of time-consuming, damage to healthy tissue, pain of the patient, risk of infection, and high cost, the biopsy examination technology , so that patients have poor compliance and are not easy to accept. In addition, before the onset of oral squamous cell carcinoma, dysplastic leukoplakia, erythema, or lichen planus usually appear first, and the degree of malignant transformation is unpredictable. Due to the complexity of tumor detection indicators, Multi-center lesions require multiple surgical biopsies for examination and diagnosis. In surgical operations, each invasive operation may affect the patient's physiological functions such as breathing, vocalization, chewing, and swallowing, which increases the patient's psychological pressure. , resulting in poor patient compliance

Method used

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  • Application of biomarker RP11-54A9.1 in prediction of oral squamous cell carcinoma and treatment of oral squamous cell carcinoma
  • Application of biomarker RP11-54A9.1 in prediction of oral squamous cell carcinoma and treatment of oral squamous cell carcinoma
  • Application of biomarker RP11-54A9.1 in prediction of oral squamous cell carcinoma and treatment of oral squamous cell carcinoma

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0057] Example 1 Screening Gene Markers Related to Oral Squamous Cell Carcinoma

[0058] 1. Sample collection

[0059] Five cases of oral squamous cell carcinoma tissues and adjacent tissues were collected, all of which were confirmed by pathological diagnosis. All patients did not receive any form of treatment before operation, and the surgically excised samples were frozen in liquid nitrogen. All the above specimens were obtained with the consent of the organizational ethics committee.

[0060] 2. Extraction of tissue RNA

[0061] Take out about 50 mg of cancer tissue and paracancerous tissue samples frozen in liquid nitrogen, put the tissue samples into a pre-cooled mortar for grinding, and transfer them to a 1.5mL EP tube when there are no large particles of tissue, and follow the kit Extract and isolate RNA according to the instructions in . The specific extraction steps are as follows:

[0062] (1) Add 1mL Trizol and let stand at room temperature for 5min;

[0063] ...

Embodiment 2

[0078] Example 2 QPCR verification of differential expression of RP11-575F12.2, RP11-54A9.1, RP11-973H7.1 and AF131217.1

[0079] 1. Organization collection

[0080] Using the collection method described in Example 1 to collect 60 oral squamous cell carcinoma tissue samples and their corresponding paracancerous tissue samples, for RP11-575F12.2, RP11-54A9.1, RP11-973H7.1 and AF131217.1 Validation of differentially expressed genes in a large sample.

[0081] 2. Extraction of tissue RNA

[0082] The extraction steps are the same as in Example 1.

[0083] 3. QPCR experiment

[0084] (1) Reverse transcription reaction

[0085] Use FastQμant cDNA First Strand Synthesis Kit (Product No.: KR106) for LncRNA reverse transcription, first remove the genomic DNA reaction, add 5×gDNA Bμffer 2.0μL, total RNA 1μg, add RNase Free ddH 2 O Bring the total volume to 10 μL and heat in a water bath at 42 °C for 3 min. Mix 10×Fast RT Bμffer 2.0μL, RT Enzyme Mix 1.0μL, FQ-RT Primer Mix 2.0μL, ...

Embodiment 3

[0113] Example 3 Silencing detection and functional verification of RP11-575F12.2, RP11-54A9.1, RP11-973H7.1 and AF131217.1

[0114] 1. Cell culture

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Abstract

The invention discloses an application of a biomarker RP11-54A9.1 in prediction of oral squamous cell carcinoma and treatment of the oral squamous cell carcinoma. In the invention, a gene RP11-54A9.1,differentially expressed in oral squamous cell carcinoma, is studied, and an influence of the differentially expressed gene RP11-54A9.1 on the oral squamous cell carcinoma is explored through a further cell experiment, it is found that proliferation and migration capacity of oral squamous cell carcinoma cells can be remarkably reduced by inhibiting expression of RP11-54A9.1, and therefore a noveloral squamous cell carcinoma biomarker with molecular diagnosis, prognosis prediction and targeted therapy is provided clinically.

Description

technical field [0001] The invention belongs to the field of biomedicine and relates to the application of a biomarker RP11-54A9.1 in predicting oral squamous cell carcinoma and its treatment. Background technique [0002] Oral squamous cell carcinoma (OSCC), as one of the most common cancers in the world, accounts for more than 90% of oral malignancies, mostly in the tongue, cheeks, gums, palate, and maxillary sinuses. There are approximately 500,000 new cases worldwide each year, and it has been on the rise in recent years. At present, it is found that the peak incidence of oral squamous cell carcinoma is between 45 and 75 years old. With the increase in the incidence of oral squamous cell carcinoma and the aging of the population, it is expected that medical institutions will face increasing pressure in the future. Oral squamous cell carcinoma is a malignant tumor with high morbidity and mortality. Although the surgical treatment, radiotherapy and chemotherapy techniques...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): C12Q1/6886A61K45/00A61K31/713A61P35/00
CPCA61K31/713A61K45/00A61P35/00C12Q1/6886C12Q2600/136C12Q2600/158C12Q2600/178
Inventor 刘欧胜周泽堃燕飞王玥陈思佳龙璐珏
Owner XIANGYA STOMATOLOGICAL HOSPITAL CENT SOUTH UNIV
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