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A kind of genetically constructed recombinant plasmid pegfp-gre-gp5gb and its application

A pegfp-gre-gp5gb, expression plasmid technology, applied in the fields of application, genetic engineering, plant gene improvement, etc., can solve problems such as undiscovered patent publications

Active Publication Date: 2022-06-07
TIANJIN AGRICULTURE COLLEGE
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

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Problems solved by technology

[0005] Through the search, no patent publications related to the patent application of the present invention have been found

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  • A kind of genetically constructed recombinant plasmid pegfp-gre-gp5gb and its application
  • A kind of genetically constructed recombinant plasmid pegfp-gre-gp5gb and its application
  • A kind of genetically constructed recombinant plasmid pegfp-gre-gp5gb and its application

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Embodiment Construction

[0039] The present invention will be further described below with reference to the examples. The following examples are descriptive and not restrictive, and the protection scope of the present invention cannot be limited by the following examples.

[0040] The raw materials used in the present invention, unless otherwise specified, are conventional commercial products, the methods used in the present invention, unless otherwise specified, are conventional methods in the field, and the quality of each substance used in the present invention is conventionally used quality.

[0041] A kind of PRRSV GP5 fragment, epitope prediction sequence GRE and PRV gB gene triple epitope gene recombination eukaryotic expression plasmid pEGFP-GRE-GP5gB, its construction step is as follows:

[0042] (1) Design and synthesis of GRE gene sequence, preferential codon optimization of PRV gB gene;

[0043] (2) Construction of recombinant shuttle vector pEGFP-GRE-GP5gB;

[0044] (3) The recombinant ...

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Abstract

The present invention relates to a recombinant eukaryotic expression plasmid pEGFP-GRE-GP5gB of PRRSV GP5 fragment, epitope prediction sequence GRE and PRV gB gene triple epitope gene, and its construction steps are as follows: (1) Design and synthesis of GRE gene sequence, PRV gB gene Preferential codon optimization; (2) construction of recombinant shuttle vector pEGFP‑GRE‑GP5gB; (3) transfection of recombinant plasmid pEGFP‑GRE‑GP5gB in Marc145 and PK15 cells, which can express the target protein, and the recombinant eukaryotic expression plasmid pEGFP‑GRE‑GP5gB was obtained GP5gB. The invention aims to construct a recombinant eukaryotic plasmid capable of preventing multiple strains of PRRSV and preventing PRV at the same time, so as to provide a certain experimental basis for the prevention and treatment of two diseases in animal husbandry.

Description

technical field [0001] The invention belongs to the technical field of genetic bioengineering, in particular to a recombinant eukaryotic expression plasmid pEGFP-GRE-GP5gB comprising a PRRSV GP5 fragment, an epitope prediction sequence GRE and a PRV gB gene triple epitope and its application. Background technique [0002] Porcine reproductive and respiratory syndrome (PRRS) and porcine pseudorabies (PR) have always plagued the healthy development of my country's pig industry. PRRSV is a swine viral disease that occurs widely in animal husbandry. Recessive infection can also cause immunosuppression and cause secondary infection. Porcine Reproductive and Respiratory Syndrome (PRRS) destroys the integrity of the immune system of pigs, thereby causing secondary infection of other diseases. PR can cause abortion, stillbirth, and mass death of newborn piglets in pregnant sows, and dyspnea and growth arrest in fattening pigs. It is also one of the major infectious diseases that en...

Claims

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Application Information

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Patent Type & Authority Patents(China)
IPC IPC(8): C12N15/85C12N15/66C12N15/40C12N15/38A61K39/12A61K39/245A61P31/14A61P31/22
CPCC12N15/85C12N15/66C07K14/005A61K39/12A61P31/14A61P31/22C12N2800/107C12N2800/22C12N2770/10022C12N2770/10034C12N2710/16722C12N2710/16734A61K2039/552A61K2039/53
Inventor 金天明李丹蓝肇煜王小月武静桥姚景丽范真玮何敬文陈婷赵微
Owner TIANJIN AGRICULTURE COLLEGE