Sea anemone co-epiphytic fungus SYSU-MS5127, fermentation compound and application of sea anemone co-epiphytic fungus and compound

A technology of SYSU-MS5127 and compounds, applied in the field of sea anemone symbiotic fungus SYSU-MS5127 and its fermented compounds and applications, can solve the decline of body defense function, human substance metabolism and water and sodium metabolism disorders, gastrointestinal side effects, etc. problems, achieving significant anti-inflammatory activity, no cytotoxicity, and high safety

Active Publication Date: 2021-03-23
SUN YAT SEN UNIV
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

However, both steroidal and non-steroidal drugs have relatively strong side effects. For example, long-term use of steroidal drugs can cause disorders in human substance metab

Method used

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  • Sea anemone co-epiphytic fungus SYSU-MS5127, fermentation compound and application of sea anemone co-epiphytic fungus and compound
  • Sea anemone co-epiphytic fungus SYSU-MS5127, fermentation compound and application of sea anemone co-epiphytic fungus and compound
  • Sea anemone co-epiphytic fungus SYSU-MS5127, fermentation compound and application of sea anemone co-epiphytic fungus and compound

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0044] Example 1 Isolation of sea anemone symbiotic fungus Fusarium graminearum SYSU-MS5127

[0045]1. Experimental sample: sea anemone from Laishizhou, Shenzhen.

[0046] 2. Experimental method:

[0047] Disinfect the surface of the fresh sea anemone, dry it slightly, grind the sea anemone, inoculate it into PDA medium under aseptic conditions, and culture it below 28°C for 5-7 days to obtain a single strain SYSU-MS5127; the obtained strain SYSU -MS5127 was stored at 4°C on the slant of PDA medium.

Embodiment 2

[0048] Example 2 Identification of the symbiotic fungus SYSU-MS5127 in sea anemones

[0049] 1. Morphological and physiological and biochemical identification:

[0050] When the bacterial strain SYSU-MS5127 obtained in Example 1 was cultured at a constant temperature of 28°C in PDA medium, the surface of the colony was white and orange-red hairy hyphae, the back was dark red, and orange-red spores were produced; the center of the mycelium protruded to form a sclerotia , grow to the four sides in a group; the initial stage of growth is white mycelium, the color of the mycelium becomes darker in the later stage, and the color of the mycelia becomes orange-red, with pink powdery things attached to the mycelia, and the plate is relatively moist.

[0051] 2. Molecular identification:

[0052] Using the CTAB method to extract the purely cultured DNA of the sea anemone symbiotic fungus SYSU-MS5127, using a pair of primers ITS1F and ITS4 in the ITS spacer region to amplify the ITS-rR...

Embodiment 3

[0053] The separation and identification of embodiment 3 compound Fusarielin M

[0054] 1. The separation of the compound Fusarielin M specifically comprises the following steps:

[0055] S1. Expanded cultivation of sea anemone symbiotic fungi: insert sea anemone symbiotic fungi into the seed medium with an inoculum size of 5 mL, culture at 28° C. and 180 rpm on a shaker for 72 hours to obtain seed culture solution; inoculate the seed culture solution to In the fermentation medium, the inoculum size is 15ml, cultured at 25°C for 28 days, filtered, and the filter residue is the sea anemone symbiotic fungal cell;

[0056] Soak the sea anemone symbiotic fungus thallus obtained from the expanded culture with methanol, filter and concentrate to obtain the extract, and the extract is sequentially mixed with 2L ethyl acetate at a volume ratio of 20%, 30%, 40%, 50%, 60%, 70% %, 80%, 90%, and 100% ethyl acetate-petroleum ether solutions were separated by gradient elution of silica gel...

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Abstract

The invention belongs to the technical field of fungus extraction compounds, and particularly relates to sea anemone co-epiphytic fungus SYSU-MS5127, a fermentation compound and application of the seaanemone co-epiphytic fungus and the compound. The sea anemone co-epiphytic fungus (Fusarium graminearum) strain SYSU-MS5127 can metabolize and synthesize the compound Fusarielin M; through experimental verification, the compound Fusarielin M can reduce the expression of inflammatory factors NO and PGE 2 in inflammatory macrophages and has remarkable anti-inflammatory activity; and experiments prove that thecompound Fusarielin M is free of cytotoxicity and high in safety, and has good application prospects in the aspect of preparing and developing anti-inflammatory medicines.

Description

technical field [0001] The invention belongs to the technical field of fungal extraction compounds. More specifically, it relates to the sea anemone symbiotic fungus SYSU-MS5127 and its fermentation compounds and applications. Background technique [0002] Inflammation is the main pathological process of many diseases. It is the defense-based response of body tissues to various damage factors. With the participation of blood vessels, body fluids and cells, a series of local changes occur in body tissues, including facilitating the removal of pathogenic substances. The response of inflammatory factors also includes the lesions that can cause their own cells, tissues and even organs. If the inflammatory response is severe, the inflammatory factors are not removed in time, and the damaged tissues or organs are not well repaired, the result of the inflammatory response will not only seriously affect people's normal life, but even pose a threat to life. For example, fibrous adh...

Claims

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Application Information

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IPC IPC(8): C12N1/14C12P7/42A61K31/19A61K36/06A61P29/00C07C51/47C07C59/46C12R1/77
CPCC12P7/42A61K36/06A61P29/00C07C51/47C07C2602/28C07C59/46Y02A50/30
Inventor 陈森华刘岚
Owner SUN YAT SEN UNIV
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