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Preparation method and use method of umbilical cord blood anticoagulant for short-time blood storage

A technology of umbilical cord blood and anticoagulant, which is applied in the field of preparation of umbilical cord blood anticoagulant for short-term blood storage, can solve problems such as cost waste, achieve the effect of improving mixing effect, avoiding low preparation efficiency, and saving costs

Inactive Publication Date: 2021-03-26
SHANDONG QILU STEM CELL ENG
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0003] Cord blood is only allowed to be transported within the province for a short period of time due to regulations, but the current relatively expensive blood preservation solution is still used when transporting umbilical cord blood, resulting in serious cost waste

Method used

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Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0020] A preparation method of an umbilical cord blood anticoagulant for short-term blood storage, comprising the following steps:

[0021] (1) In parts by weight, add 3.31-3.32 parts of trisodium citrate and 1.50-1.56 parts of diphosphoglyceric acid into 98-100 parts of distilled water. After mixing and stirring, use ultrasonic treatment for 10-15 minutes, wherein ultrasonic treatment The frequency is 20-25kHz, and the ultrasonic treatment time is 15-20min;

[0022] (2) In parts by weight, continue to add 0.01-0.05 parts of citric acid and 0.08-0.14 parts of fructose diphosphate to the mixed solution obtained in step (1), and mix and stir evenly;

[0023] (3) Add the mixed solution prepared in step (2) into the blood collection bag, and after sterilization, the cord blood anticoagulant is obtained, wherein the temperature of the sterilization treatment is 115°C, and the time of the sterilization treatment is 2-3min .

[0024] The invention also discloses a method for using ...

Embodiment 2

[0026] A preparation method of an umbilical cord blood anticoagulant for short-term blood storage, comprising the following steps:

[0027] (1) In parts by weight, add 3.31-3.32 parts of trisodium citrate and 1.50-1.56 parts of diphosphoglyceric acid into 98-100 parts of distilled water. After mixing and stirring, use ultrasonic treatment for 10-15 minutes, wherein ultrasonic treatment The frequency is 20-25kHz, and the ultrasonic treatment time is 15-20min;

[0028] (2) In parts by weight, continue to add 0.01-0.05 parts of citric acid and 0.08-0.14 parts of fructose diphosphate to the mixed solution obtained in step (1), and mix and stir evenly;

[0029] (3) Add the mixed solution prepared in step (2) into the blood collection bag, and after sterilization, the cord blood anticoagulant is obtained, wherein the temperature of the sterilization treatment is 115°C, and the time of the sterilization treatment is 2-3min .

[0030] The invention also discloses a method for using ...

Embodiment 3

[0032] A preparation method of an umbilical cord blood anticoagulant for short-term blood storage, comprising the following steps:

[0033] (1) In parts by weight, add 3.31-3.32 parts of trisodium citrate and 1.50-1.56 parts of diphosphoglyceric acid into 98-100 parts of distilled water. After mixing and stirring, use ultrasonic treatment for 10-15 minutes, wherein ultrasonic treatment The frequency is 20-25kHz, and the ultrasonic treatment time is 15-20min;

[0034] (2) In parts by weight, continue to add 0.01-0.05 parts of citric acid and 0.08-0.14 parts of fructose diphosphate to the mixed solution obtained in step (1), and mix and stir evenly;

[0035] (3) Add the mixed solution prepared in step (2) into the blood collection bag, and after sterilization, the cord blood anticoagulant is obtained, wherein the temperature of the sterilization treatment is 115°C, and the time of the sterilization treatment is 2-3min .

[0036] The invention also discloses a method for using ...

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PUM

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Abstract

The invention discloses a preparation method of an umbilical cord blood anticoagulant for short-time blood storage, which comprises the following steps: (1) adding trisodium citrate and diphosphoglyceric acid into distilled water, mixing and stirring, and carrying out ultrasonic treatment for 10-15 minutes, (2) continuously adding citric acid and fructose diphosphate into the mixed solution obtained in the step (1), and uniformly mixing and stirring, and (3) adding the mixed solution prepared in the step (2) into a blood sampling bag, and sterilizing to obtain the umbilical cord blood anticoagulant. Compared with the traditional blood anticoagulant, the umbilical cord blood anticoagulant for short-time blood storage provided by the invention has the advantages that the use of glucose is reduced, after a blood sample is collected and mixed with the umbilical cord blood anticoagulant disclosed by the invention, tests and actual use prove that the preservation life of lymphocytes after anticoagulation of human external blood can reach 5 days, and the activity of the lymphocytes is kept.

Description

technical field [0001] The invention belongs to the technical field of short-term blood storage, and in particular relates to a preparation method and a use method of an umbilical cord blood anticoagulant for short-term blood storage. Background technique [0002] The main components of the current blood preservation solution are citrate, glucose, phosphate and adenine. According to different formulas, it is divided into two categories: ACD and CPD. The difference between the two is that CPD is added with adenine and phosphate, so it can prolong the storage period of red blood cells to 35 days and enhance the oxygen release function of red blood cells. Glucose in the solution is an essential nutrient for red blood cell metabolism, which can prolong the storage time of red blood cells and prevent hemolysis; it can also slow down the disappearance of organic phosphorus in cells and prevent damage to red blood cell storage. [0003] Cord blood is only allowed to be transported...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): A01N1/02
CPCA01N1/021
Inventor 雒猛侯怀水高峰刘庆凯
Owner SHANDONG QILU STEM CELL ENG
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