Method for identifying deer hard antler button effective components for treating mastitis

A technology of effective components and identification methods, which is applied in the field of identification of effective components, can solve the problems of not screening active components, not being able to better reflect the comprehensive effect of animal-derived Chinese medicine components, ignoring the correlation of drug components, etc., to achieve improved reliability Effects of Reliability and Accuracy

Pending Publication Date: 2021-04-06
长春科技学院
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  • Claims
  • Application Information

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Problems solved by technology

[0003] Throughout modern medicine and scholars' scientific research on antler discs, for mastitis, so far, no pharmacological experiments and anti-inflammatory mechanism research have been conducted in strict scientific experiments, and no effective ingredients have been screened. Most of the existing studies on the efficacy of antler discs focus on the analysis and research of single components such as proteins and polypeptides. The corresponding extraction methods are also based on the chemical properties of the monomer components, selecting suitable solvents and conditions for extracti

Method used

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  • Method for identifying deer hard antler button effective components for treating mastitis
  • Method for identifying deer hard antler button effective components for treating mastitis
  • Method for identifying deer hard antler button effective components for treating mastitis

Examples

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Embodiment 1

[0061] A method for identifying an effective component of antler discs for treating mastitis, comprising the following steps:

[0062] S1 sets the antler disc extract with gradient concentration;

[0063] S2 The obtained gradient concentration of antler disc extract was used for immunological experiments on mice, the mammary gland tissues of mice at different stages during the experiment were extracted, the mammary gland epithelial cells were separated, and the obtained mammary gland epithelial cells were subjected to enzyme linkage Immuno-real-time fluorescence detection to determine and screen the changing levels of inflammatory cytokines;

[0064] S3 For the level of change of the screened inflammatory cytokines, use the fingerprints and simultaneously perform gray correlation analysis to determine and screen out the peak effect, and collect the treatment solution corresponding to the peak effect;

[0065] S4 Verifies the single-component peaks corresponding to the obtaine...

Embodiment 2

[0117] Change boiling time in pretreatment to be 2h, all the other steps and formula are identical, compare with embodiment 1, deviation P<0.02.

Embodiment 3

[0119] Change boiling time in pretreatment to be 6h, all the other steps and formula are identical, compare with embodiment 1, deviation P<0.04.

[0120] Contrast by above-mentioned embodiment 1 and embodiment 2,3, can prove the superiority of the present invention.

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Abstract

The invention discloses a method for identifying deer hard antler button effective components for treating mastitis. The method comprises the following steps: S1, setting deer hard antler button extracts with gradient concentration; S2, determining and screening the change level of inflammatory cytokines; S3, determining and screening out an effect peak value, and collecting a treating fluid corresponding to the effect peak value; S4, verifying a single-component peak, and determining the treating fluid with a verified effect peak value; and S5, determining chemical components of the effective components. According to the invention, deer hard antler button is selected, and detailed components are obtained through pretreatment, concentration gradient setting, immunoassay, enzyme-linked immunosorbent assay, real-time fluorescent quantitative PCR detection, immunoblotting analysis, fingerprint map, grey correlation analysis, verification of a single-component peak and non-targeted metabonomics of a liquid chromatograph-mass spectrometer, so that comprehensive judgment of efficacy and accurate identification are realized.

Description

technical field [0001] The invention relates to the technical field of identification of traditional Chinese medicine components, in particular to an identification method for effective components of antler discs for treating mastitis. Background technique [0002] Antler discs are the antler bases that fall off in the spring of the next spring after red deer or sika deer saw antlers. It contains mineral elements, amino acids, proteins, polypeptides, polysaccharides, lipids, cholesterol and sex hormones. In traditional animal Chinese medicine, deer antler discs have a long history of medicinal use. It not only has the effects of warming kidney yang, strengthening muscles and bones, promoting blood circulation and reducing swelling, but also has the magic of no inflammation, no suppuration, and no swelling from the deer horn discs that fall off naturally on living tissues. Biological phenomena and extensive folk applications all show that antler discs have good anti-inflammat...

Claims

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Application Information

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IPC IPC(8): G01N33/68G01N30/02C12Q1/6851
CPCG01N33/6863G01N30/02C12Q1/6851G01N2030/027
Inventor 关晴李春义姚梦杰
Owner 长春科技学院
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