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Particle sorting method and particle sorting equipment

A sorting and particle technology, applied in individual particle analysis, particle and sedimentation analysis, measuring devices, etc., can solve the problems of unable to obtain the shape of sorted cells, unable to obtain large samples, misselected statistical data, etc., to achieve increased Particle detection means, improving the accuracy of particle sorting, and analyzing the effect of improving the sorting accuracy

Pending Publication Date: 2021-04-20
SHANGHAI MICRO ELECTRONICS EQUIP (GRP) CO LTD
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Problems solved by technology

[0003] In the prior art, when the particles to be tested are sorted by flow cytometry equipment, the cell composition is usually judged by obtaining fluorescence and scattered light energy, and then the cells are sorted according to the cell composition data. However, the existing flow cytometry equipment cannot obtain the sorted cells. The cell morphology needs to be obtained by observing the sorted cells on a fluorescence microscope, so it is time-consuming and laborious, and the statistical data of large samples cannot be obtained, which may easily lead to misselection

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  • Particle sorting method and particle sorting equipment
  • Particle sorting method and particle sorting equipment
  • Particle sorting method and particle sorting equipment

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Embodiment Construction

[0066] The present invention will be further described in detail below in conjunction with the accompanying drawings and embodiments. It should be understood that the specific embodiments described here are only used to explain the present invention, but not to limit the present invention. In addition, it should be noted that, for the convenience of description, only some structures related to the present invention are shown in the drawings but not all structures.

[0067] At present, the following steps are usually used to sort particles by flow cytometry:

[0068] a. The particles (cells, bacteria, etc.) to be stained are sent into the sample tube;

[0069] b. Divide the sample tube into multiple parts (greater than or equal to 2 parts);

[0070] c. Put one of the sample tubes into the flow cytometry device;

[0071] d. Flow cytometry sorting equipment analyzes the composition of particles; identifies the sorting gate;

[0072] e. Manually, semi-automatically, or fully aut...

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Abstract

The embodiment of the invention discloses a particle sorting method and particle sorting equipment. The particle sorting method comprises the steps of carrying out first optical signal detection on a scattered light signal and a fluorescence signal which are generated after particles to be detected in a liquid flow chamber are irradiated by detection light, and carrying out imaging detection on the particles to be detected; analyzing the first optical signal detection result and / or the imaging detection result, and determining a sorting instruction; and sorting the to-be-detected particles according to the sorting instruction. According to the scheme of the embodiment of the invention, the sorting efficiency and sorting accuracy are improved.

Description

technical field [0001] Embodiments of the present invention relate to particle analysis and sorting technologies, and in particular, to a particle sorting method and particle sorting equipment. Background technique [0002] Flow cytometry is a typical particle sorting device that relies on the flow of cells or other particles in a fluid flow stream to determine one or more characteristics of the particle under study. For example, a liquid sample containing cells is passed through a flow cytometer in a fast-moving stream such that each cell passes through the sensing region consecutively and one at a time. As each cell passes through the sensing region, if the probe beam is directed toward the area, the cells scatter that light as they pass through it. In addition, the excitation energy of the probe beam can excite the labeled cells to fluoresce, which can be detected to identify specifically labeled cells. [0003] In the prior art, when the particles to be tested are sort...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): G01N15/14
Inventor 蓝科
Owner SHANGHAI MICRO ELECTRONICS EQUIP (GRP) CO LTD
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