Application of ECCA in preparation of anti-tumor drugs

An anti-tumor and tumor technology, applied in the field of biomedicine, can solve problems such as low p53 mutation rate

Active Publication Date: 2021-04-30
SHANDONG UNIV
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

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Problems solved by technology

However, a relatively low rate of p53 mutations (less than 20%) has been found in human melanomas, which predominantly harbor oncogenic BRAF mutations

Method used

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  • Application of ECCA in preparation of anti-tumor drugs
  • Application of ECCA in preparation of anti-tumor drugs
  • Application of ECCA in preparation of anti-tumor drugs

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Experimental program
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experiment example

[0051] 1. Materials and methods

[0052] 1.1 Reagents

[0053] ECCA stock solutions (50 mM) were prepared by dissolving the compounds in dimethyl sulfoxide (DMSO), then diluted with DMEM medium to prepare working solutions at the concentrations indicated in the graphs of in vitro study results, and diluted with phosphate-buffered saline (PBS ) was diluted to a concentration of 7mM for in vivo studies.

[0054] 1.2 Cell lines and culture

[0055] Primary human melanocytes were isolated from adult skin tissue, and were isolated and cultured according to the methods reported in the literature (Mi, J. et al. A ROCK inhibitor promotes keratinocyte survival and paracrine secretion, enhancing establishment of primary human melanocytes and melanocyte–keratinocyte co -cultures. Pigment Cell & Melanoma Research 33(2020).). Melanoma cell lines Mel-JUSO, A375 and UACC62 were cultured in DMEM (Thermo Fisher Scientific): F-12 (Thermo Fisher Scientific) medium containing 10% fetal bovine ...

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Abstract

The invention provides an application of ECCA in preparation of an anti-tumor drug. According to the invention, experiment results prove that 9-ethyl-9H-carbazole-3-formaldehyde (ECCA) can inhibit the growth of melanoma cells and induce the apoptosis of the melanoma cells in vitro and in vitro, has high anti-melanoma activity, has no toxic or side effect on normal tissue cells of a human body, and has the potential of being developed into the medicine for treating melanoma.

Description

technical field [0001] The application relates to the field of biomedicine, in particular to the application of ECCA in the preparation of antitumor drugs. Background technique [0002] The information disclosed in this background section is only intended to increase the understanding of the general background of the application, and is not necessarily regarded as an acknowledgment or any form of suggestion that the information constitutes the prior art already known to those skilled in the art. [0003] Cutaneous melanoma is the most dangerous and aggressive type of skin cancer, accounting for more than 75% of skin cancer-related deaths. Current strategies for the treatment of melanoma include surgery, chemotherapy, radiotherapy, and immunotherapy. However, for patients with advanced melanoma, most traditional therapies often have poor clinical effects, and one of the main reasons is the chemoresistance to targeted therapy applications. Therefore, there is an urgent need ...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): A61K31/404A61K45/06A61P35/00
CPCA61K31/404A61K45/06A61P35/00
Inventor 吴训伟赵宝祥苗俊英温洁白福响冷雪
Owner SHANDONG UNIV
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