The invention discloses an antibiotic-free premix of a late-stage feed for dry-raised meat ducks. The antibiotic-free premix comprises the following raw materials: compound multi-minerals, compound multi-vitamins, compound enzymes, compound acidifying agent, macleaya cordata powder, probiotic agent, gamma-aminobutyric acid, immune polysaccharides, tributyrin, 98.5% lysine, 99% methionine, threonine, tryptophan, sodium chloride, 50% choline chloride and carrier. The application method of the antibiotic-free premix comprises the following steps: adding 1-3% of the antibiotic-free premix to basic materials, mixing with the basic materials, and carrying out granulation. The macleaya cordata powder and the immune polysaccharide are used as main raw materials, supplemented by the probiotic agent, the compound enzymes, the acidifying agent, the tributyrin and the gamma-aminobutyric acid, so that antibiotics can be completely replaced, wherein the macleaya cordata powder, the tributyrin and the gamma-aminobutyric acid have an obvious synergistic effect. The antibiotic-free premix of the invention can be used for producing green, environment-friendly and healthy livestock products.