Method for inhibiting growth of gibberella saubinetii and reducing toxins of wheat scab infected grains and application of method
A technology of wheat scab and gibberella, applied in the field of plant pest control, can solve the problems of pesticide residues, affecting the control effect, lack and other problems, and achieve the effects of delaying the expansion speed, reducing the rate of diseased spikelets, and high safety
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Embodiment 1
[0020] Embodiment 1: in vitro experiment
[0021] The invention provides a method for inhibiting the growth of gibberella and reducing the grain toxin of wheat scab. The inhibitory effect of Gibberella, which causes wheat head blight, was identified in vitro. Including the following steps:
[0022] (1) Prepare a selenium preparation solution; the selenium preparation is an organic selenomethionine solution, and the organic selenomethionine solution refers to a solution with a concentration of 50 ppm. Clear water was used as a control group;
[0023] (2) Add the above concentration of selenium preparation solution to the toxin induction medium as the treatment group, and add the same amount of water as the control group; take a piece of bacterial block from the activated gibberella strain solid plate medium with a disc sampler and place In the middle of the toxin induction medium;
[0024] (3) Record the mycelial growth diameters of the treatment group and the control group...
Embodiment 2
[0032] Embodiment 2: in vivo experiment
[0033] The invention provides a method for inhibiting the growth of gibberella and reducing the grain toxin of wheat scab. Under field conditions, artificial inoculation of scab single flower drop method is carried out on the moderately resistant scab variety NIL-7 at the flowering stage For identification, spray selenium solution immediately after inoculation to ensure the evaluation of scab control effect under the condition of gibberella infestation. Including the following steps:
[0034] (1) Prepare a selenium preparation solution; the selenium preparation is an organic selenomethionine solution. The described organic selenomethionine solution refers to a solution with a concentration of 50 ppm.
[0035] (2) Use a disk sampler from the activated gibberella strain solid plate medium, take 3-5 pieces of bacteria and put them into 150mL of sterilized mung bean soup medium, and then place them on a shaker, at 180rpm, the temperature...
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