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Precise RNA concentration detection method and device, equipment, medium and application

A concentration detection and precise technology, applied in the field of spectrum detection, can solve the problems of high cost, error, high RNA concentration, etc.

Pending Publication Date: 2021-05-25
广州睿贝医学科技有限公司
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

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Problems solved by technology

[0004] However, when there is pollution, such as protein pollution, it will produce interference light absorption at a wavelength of 260nm, resulting in a high concentration of RNA, resulting in serious errors
In the prior art, if the concentration of RNA needs to be obtained accurately, it is necessary to purify the protein-contaminated RNA, and then measure the purified RNA, which is complicated and costly

Method used

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  • Precise RNA concentration detection method and device, equipment, medium and application
  • Precise RNA concentration detection method and device, equipment, medium and application
  • Precise RNA concentration detection method and device, equipment, medium and application

Examples

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Embodiment 1

[0076] see figure 1 , figure 1 It is a schematic flowchart of a precise RNA concentration detection method disclosed in the embodiment of the present invention. Wherein, the execution subject of the method described in the embodiment of the present invention is built by software / hardware, and can be applied to optical density detectors, etc. Of course, devices with processing and storage functions such as mobile phones, tablet computers, and servers can also be used. Such as figure 1 As shown, the precise RNA concentration detection method comprises the following steps:

[0077] 110. Receive the total optical density of collected pollutants at wavelengths of 260 nm, 280 nm and 320 nm, where the pollutants are RNA containing protein.

[0078] The instrument for collecting optical density may be an optical density detector such as a spectrophotometer. Use an optical density detector to measure the spectrum of pollutants, the wavelength range is 220nm–350nm, and the step is 1...

Embodiment 2

[0127] see figure 2 , figure 2 It is a structural schematic diagram of a precise RNA concentration detection device disclosed in the embodiment of the present invention. Such as figure 2 As shown, the precise RNA concentration detection device may include:

[0128] The receiving unit 210 is configured to receive the total optical density of the collected pollutants at wavelengths of 260nm, 280nm and 320nm, the pollutants being RNA containing protein;

[0129] The decomposing unit 220 is configured to subtract the background reference in the total optical density to obtain the basic total optical density, and decompose the basic total optical density:

[0130] △OD260 Total =△OD260 RNA +△OD260 PRO (19)

[0131] △OD280 Total =△OD280 PRO +△OD280 RNA (20)

[0132] Among them, △OD260 Total and △OD280 Total Respectively, the basic total optical density of pollutants at wavelengths of 260nm and 280nm, △OD260 RNA and △OD260 PRO Respectively, the basic optical densit...

Embodiment 3

[0142] see image 3 , image 3 It is a schematic structural diagram of an electronic device disclosed in an embodiment of the present invention. Such as image 3 As shown, the electronic equipment may include:

[0143] a memory 310 storing executable program code;

[0144] a processor 320 coupled to the memory 310;

[0145] Wherein, the processor 320 invokes the executable program code stored in the memory 310 to execute some or all of the steps in the method for detecting the precise RNA concentration in the first embodiment.

[0146] The embodiment of the present invention discloses a computer-readable storage medium, which stores a computer program, wherein the computer program enables the computer to execute some or all of the steps in the method for detecting the precise RNA concentration in the first embodiment.

[0147] The embodiment of the present invention also discloses a computer program product, wherein, when the computer program product is run on the compute...

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Abstract

The embodiment of the invention relates to the technical field of spectrum detection, and discloses a precise RNA concentration detection method and device, equipment, a medium and an application. The method comprises the steps: receiving total optical densities of collected pollutants at wavelengths of 260 nm, 280 nm and 320 nm; deducting a background reference in the total optical densities to obtain a basic total optical density, and decomposing the basic total optical density; rewriting the formula according to the proportional constants [delta]OD280RNA% and [delta]OD260PRO%, and obtaining the [delta]OD260RNA and the [delta]OD280PRO; and calculating the concentration M of RNA and the concentration N of protein in the pollutants according to the [delta]OD260RNA, the [delta]OD280PRO and the [delta]OD260PRO%. By implementing the embodiment of the invention, the comprehensive spectrum generated by the RNA and the protein in the same pollutant is split, so that the aim of simultaneously detecting the RNA and the protein is fulfilled.

Description

technical field [0001] The invention relates to the technical field of spectral detection, in particular to a precise RNA concentration detection method, device, equipment, medium and application. Background technique [0002] In biological research, especially molecular biology research, the concentration detection of RNA is often involved. The traditional RNA concentration detection method adopts the method of ultraviolet light absorption, and measures the light absorption (that is, optical density, denoted as OD260) of RNA with a light path of 10mm at a wavelength of 260nm, and calculates the RNA concentration by OD260: [0003] RNA concentration=40*OD260 (unit: ng / ul) [0004] However, when there is pollution, such as protein pollution, interference light absorption will occur at a wavelength of 260nm, resulting in high RNA concentration and serious errors. In the prior art, if the concentration of RNA needs to be obtained accurately, it is necessary to purify the prot...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): G01N21/25G01N21/27G01N21/31
CPCG01N21/25G01N21/31G01N21/274
Inventor 公杰张俊宾郭求真谭卿
Owner 广州睿贝医学科技有限公司
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