Accurate DNA concentration detection method, device and equipment, medium and application

A concentration detection and precise technology, applied in the field of spectrum detection, can solve problems such as errors, high DNA concentration, and high cost
CN112710620APending Publication Date: 2021-04-27广州睿贝医学科技有限公司
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Patent Information

Authority / Receiving Office
CN · China
Patent Type
Applications(China)
Current Assignee / Owner
广州睿贝医学科技有限公司
Publication Date
2021-04-27

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Abstract

The embodiment of the invention relates to the technical field of spectrum detection, and discloses an accurate DNA concentration detection method, device and equipment, a medium and application. The method comprises the following steps: receiving the total optical densities of collected pollutants at wavelengths of 260 nm, 280 nm and 320 nm; deducting a background reference in the total optical density to obtain a basic total optical density, and decomposing the basic total optical density; re-writting the formula according to the proportional constant delta OD280DNA% and the proportional constant delta OD260PRO% to obtain thedelta OD260DNA and delta OD280PRO; and calculating the concentration M of the DNA and the concentration N of the protein in the pollutants according to the delta OD260DNA, the delta OD280PRO and the delta OD260PRO%. By implementing the embodiment of the invention, the comprehensive spectrum generated by DNA and protein in the same pollutant is split, so that the purpose of simultaneously detecting DNA and protein is achieved.
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Description

technical field

[0001] The invention relates to the technical field of spectral detection, in particular to a precise DNA concentration detection method, device, equipment, medium and application. Background technique

[0002] In biological research, especially molecular biology research, the concentration detection of DNA is often involved. The traditional DNA concentration detection method adopts the method of ultraviolet light absorption, and measures the light absorption of DNA with a 10mm optical path at a wavelength of 260nm (that is, optical density, recorded as OD260), and calculates the DNA concentration by OD260:

[0003] DNA concentration=50*OD260 (unit: ng / ul)

[0004] However, when there is pollution, such as protein pollution, interference light absorption will occur at a wavelength of 260nm, resulting in high DNA concentration and serious errors. In the prior art, if it is necessary to accurately obtain the concentration of DNA, it is necessary to purify the...

Claims

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