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Accurate DNA concentration detection method, device and equipment, medium and application

A concentration detection and precise technology, applied in the field of spectrum detection, can solve problems such as errors, high DNA concentration, and high cost

Pending Publication Date: 2021-04-27
广州睿贝医学科技有限公司
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

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Problems solved by technology

[0004] However, when there is pollution, such as protein pollution, it will produce interference light absorption at a wavelength of 260nm, resulting in high DNA concentration and serious errors
In the prior art, if the concentration of DNA needs to be obtained accurately, it is necessary to purify the protein-contaminated DNA, and then measure the purified DNA, which is complicated and costly

Method used

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  • Accurate DNA concentration detection method, device and equipment, medium and application
  • Accurate DNA concentration detection method, device and equipment, medium and application
  • Accurate DNA concentration detection method, device and equipment, medium and application

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Embodiment 1

[0076] see figure 1 , figure 1 It is a schematic flow chart of a precise DNA concentration detection method disclosed in the embodiment of the present invention. Wherein, the execution subject of the method described in the embodiment of the present invention is built by software / hardware, and can be applied to optical density detectors, etc. Of course, devices with processing and storage functions such as mobile phones, tablet computers, and servers can also be used. like figure 1 As shown, the precise DNA concentration detection method comprises the following steps:

[0077] 110. Receive the total optical density of collected pollutants at wavelengths of 260 nm, 280 nm, and 320 nm, where the pollutants are DNA containing protein.

[0078] The instrument for collecting optical density may be an optical density detector such as a spectrophotometer. Use an optical density detector to measure the spectrum of pollutants, the wavelength range is 220nm–350nm, and the step is 1n...

Embodiment 2

[0127] see figure 2 , figure 2 It is a structural schematic diagram of a precise DNA concentration detection device disclosed in the embodiment of the present invention. like figure 2 As shown, the precise DNA concentration detection device may include:

[0128] The receiving unit 210 is configured to receive the total optical density of the collected pollutants at wavelengths of 260nm, 280nm and 320nm, the pollutants being DNA containing proteins;

[0129] Decomposition unit 220, configured to subtract the background reference in the total optical density to obtain the basic total optical density, and decompose the basic total optical density:

[0130] △OD260 Total =△OD260 DNA +△OD260 PRO (19)

[0131] △OD280 Total =△OD280 PRO +△OD280 DNA (20)

[0132] Among them, △OD260 Total and △OD280 Total Respectively, the basic total optical density of pollutants at wavelengths of 260nm and 280nm, △OD260 DNA and △OD260 PRO are the basic optical densities of DNA and p...

Embodiment 3

[0143] see image 3 , image 3 It is a schematic structural diagram of an electronic device disclosed in an embodiment of the present invention. like image 3 As shown, the electronic equipment may include:

[0144] a memory 310 storing executable program code;

[0145] a processor 320 coupled to the memory 310;

[0146] Wherein, the processor 320 invokes the executable program code stored in the memory 310 to execute some or all of the steps in the precise DNA concentration detection method in the first embodiment.

[0147] The embodiment of the present invention discloses a computer-readable storage medium, which stores a computer program, wherein the computer program enables the computer to execute part or all of the steps in the precise DNA concentration detection method in the first embodiment.

[0148] The embodiment of the present invention also discloses a computer program product, wherein, when the computer program product is run on the computer, the computer is ...

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Abstract

The embodiment of the invention relates to the technical field of spectrum detection, and discloses an accurate DNA concentration detection method, device and equipment, a medium and application. The method comprises the following steps: receiving the total optical densities of collected pollutants at wavelengths of 260 nm, 280 nm and 320 nm; deducting a background reference in the total optical density to obtain a basic total optical density, and decomposing the basic total optical density; re-writting the formula according to the proportional constant delta OD280DNA% and the proportional constant delta OD260PRO% to obtain thedelta OD260DNA and delta OD280PRO; and calculating the concentration M of the DNA and the concentration N of the protein in the pollutants according to the delta OD260DNA, the delta OD280PRO and the delta OD260PRO%. By implementing the embodiment of the invention, the comprehensive spectrum generated by DNA and protein in the same pollutant is split, so that the purpose of simultaneously detecting DNA and protein is achieved.

Description

technical field [0001] The invention relates to the technical field of spectral detection, in particular to a precise DNA concentration detection method, device, equipment, medium and application. Background technique [0002] In biological research, especially molecular biology research, the concentration detection of DNA is often involved. The traditional DNA concentration detection method adopts the method of ultraviolet light absorption, and measures the light absorption of DNA with a 10mm optical path at a wavelength of 260nm (that is, optical density, recorded as OD260), and calculates the DNA concentration by OD260: [0003] DNA concentration=50*OD260 (unit: ng / ul) [0004] However, when there is pollution, such as protein pollution, interference light absorption will occur at a wavelength of 260nm, resulting in high DNA concentration and serious errors. In the prior art, if it is necessary to accurately obtain the concentration of DNA, it is necessary to purify the...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): G01N21/33
CPCG01N21/33
Inventor 公杰张俊宾郭求真谭卿
Owner 广州睿贝医学科技有限公司
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