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Method for constructing pH distinguishing colorimetric biosensor

A biosensor and colorimetric technology, applied in the field of pH-resolution colorimetric biosensors, can solve problems such as adverse effects on humans, animals and crops, diseases and economic losses, global food pollution, etc.

Active Publication Date: 2018-09-28
JIANGSU UNIV
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0004] Global contamination of food by mycotoxins is a major concern as they adversely affect humans, animals and crops, leading to disease and economic loss

Method used

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  • Method for constructing pH distinguishing colorimetric biosensor
  • Method for constructing pH distinguishing colorimetric biosensor
  • Method for constructing pH distinguishing colorimetric biosensor

Examples

Experimental program
Comparison scheme
Effect test

example 1

[0108] 1) Ferrite oxide nanoparticles and graphene oxide composite (Fe 3 O 4 / GO) Preparation

[0109] Firstly, 50mL graphene oxide aqueous solution (0.8mg / mL) was sonicated for 1h and nitrogen gas was applied for 30min; then under the protection of nitrogen, 50mL of ferric chloride (0.055g) and ferrous sulfate (0.048g) mixed solution was added dropwise and stirred for 12h . Stir vigorously, add 6M sodium hydroxide dropwise to the mixture until the pH value reaches 10.0, react at 90°C for 1.5 hours, and then cool to room temperature. Wash with pH 7.4 phosphate buffer solution until it is neutral, and store at a constant volume with 100 mL at 4°C for later use.

[0110] 2) Colorimetric analysis at different temperatures

[0111] The fixed reaction time is 90min, and 1mL PP-DNA is pipetted separately 1 -GO-OTA aptamer-DNA 2 -Fe 3 O 4 / GO, MGCB-DNA 3 -GO-AFB1aptamer-DNA 4 -Fe 3 O 4 / GO solution mix, remove the supernatant after magnetic separation, add 1mL target ochratoxin A (OTA) an...

example 2

[0113] 1) Ferrite oxide nanoparticles and graphene oxide composite (Fe 3 O 4 / GO) Preparation

[0114] Firstly, 50mL graphene oxide aqueous solution (0.8mg / mL) was sonicated for 1h and nitrogen gas was applied for 30min; then under the protection of nitrogen, 50mL of mixed solution of ferric chloride (0.055g) and ferrous sulfate (0.048g) was added dropwise and stirred for 12h . Stir vigorously, add 6M sodium hydroxide dropwise to the mixture until the pH value reaches 10.0, react at 90°C for 1.5 hours, and then cool to room temperature. Wash with pH 7.4 phosphate buffer solution until it is neutral, and store at a constant volume with 100 mL at 4°C for later use.

[0115] 2) Colorimetric analysis at different times

[0116] The fixed reaction temperature was 37°C. , Respectively pipette 1mL PP-DNA 1 -GO-OTA aptamer-DNA 2 -Fe 3 O 4 / GO, MGCB-DNA 3 -GO-AFB1aptamer-DNA 4 -Fe 3 O 4 / GO solution mix, remove the supernatant after magnetic separation, add 1mL target ochratoxin A (OTA) an...

example 3

[0118] 1) Fe3O3 nanoparticles and graphene oxide composite (Fe 3 O 4 / GO) Preparation

[0119] Firstly, 50mL graphene oxide aqueous solution (0.8mg / mL) was sonicated for 1h and nitrogen gas was applied for 30min; then under the protection of nitrogen, 50mL of ferric chloride (0.055g) and ferrous sulfate (0.048g) mixed solution was added dropwise and stirred for 12h . Stir vigorously, add 6M sodium hydroxide dropwise to the mixture until the pH value reaches 10.0, react at 90°C for 1.5 hours, and then cool to room temperature. Wash with pH 7.4 phosphate buffer solution until it is neutral, and store at a constant volume with 100 mL at 4°C for later use.

[0120] 2) Colorimetric detection of ochratoxin A (OTA) and aflatoxin B1 (AFB1)

[0121] The fixed reaction temperature was 37°C and the reaction time was 90 min. , Respectively pipette 1mL PP-DNA 1 -GO-OTAaptamer-DNA 2 -Fe 3 O 4 / GO, MGCB-DNA 3 -GO-AFB1aptamer-DNA 4 -Fe 3 O 4 / GO solution mix, remove the supernatant after magnetic...

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Abstract

The invention belongs to the field of construction of a colorimetric biosensor, and provides a method for constructing a pH distinguishing colorimetric biosensor. A technical scheme comprises the following steps: 1) preparing ferriferrous oxide nano particles and a graphene oxide compound (Fe3O4 / GO); and 2) constructing the pH distinguishing colorimetric biosensor. The pH distinguishing colorimetric biosensor provides a simple, rapid, accurate and low-cost strategy for multi-target detection.

Description

Technical field [0001] The invention belongs to the field of colorimetric biosensor construction, and specifically relates to a method for constructing a simple, accurate, low-cost and pH-resolved colorimetric biosensor for simultaneous detection of multiple targets. Background technique [0002] Colorimetric sensors have been widely used in daily life to detect various targets, such as DNA, proteins, cells, toxins, heavy metal ions, etc., due to their low cost, convenient carrying, and simple operation. The main reason is that colorimetric sensing does not require complicated instruments, and the quantification of the target can be achieved by the naked eye, so it is very suitable for test points or field tests. [0003] For colorimetric sensors, the current design is mainly based on a single color change, which severely limits the ability to detect multiple targets. So far, there are few reports about colorimetric biosensors that can detect multiple targets. Color-changing dyes...

Claims

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Application Information

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IPC IPC(8): G01N21/80
CPCG01N21/80
Inventor 郝楠王坤周舟
Owner JIANGSU UNIV
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