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A method for the rapid detection of aromatic pollutants in wastewater using a nanoporous gold horseradish peroxidase sensor

A technology of golden horseradish peroxide and horseradish peroxidase is applied in the field of detecting aromatic pollutants in wastewater, and achieves the effects of cumbersome solution steps, broad application prospects and reduced measurement standard deviation.

Active Publication Date: 2018-02-23
SHANDONG UNIV
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0014] Aiming at the deficiencies of the existing detection methods, the problem to be solved in the present invention is to provide a method for rapidly detecting aromatic pollutants in wastewater using a nanoporous gold horseradish peroxidase sensor

Method used

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  • A method for the rapid detection of aromatic pollutants in wastewater using a nanoporous gold horseradish peroxidase sensor
  • A method for the rapid detection of aromatic pollutants in wastewater using a nanoporous gold horseradish peroxidase sensor
  • A method for the rapid detection of aromatic pollutants in wastewater using a nanoporous gold horseradish peroxidase sensor

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0034] Example 1: Preparation of a nanoporous gold horseradish peroxidase sensor electrode

[0035] (1) Preparation of nanoporous gold: place an Au / Ag alloy sheet with a thickness of 100 nm in pure nitric acid, and corrode it for 20 minutes at a temperature of 25° C. to prepare a nanoporous gold film;

[0036] (2) enzyme immobilization: horseradish peroxidase is dissolved in pH 7.0 concentration 50mM phosphate buffered saline, and the obtained concentration is 10mg / mL enzyme solution; The root peroxidase is attached and fixed on the surface of the nanoporous gold film, and the fixation time is 16 hours;

[0037] (3) Electrode preparation: paste the horseradish peroxidase / nanoporous gold complex prepared in step (2) on the surface of the glassy carbon electrode, and dry it in vacuum for 1.5 hours to prepare the horseradish peroxidase / nanoporous gold complex Gold / glassy carbon electrodes.

Embodiment 2

[0038] Example 2: Preparation of a nanoporous gold horseradish peroxidase sensor electrode

[0039] (1) Preparation of nanoporous gold: place an Au / Ag alloy sheet with a thickness of 100 nm in pure nitric acid, and corrode it for 30 minutes at a temperature of 20° C. to prepare a nanoporous gold film;

[0040] (2) Enzyme immobilization: Dissolve horseradish peroxidase in pH 7.0 concentration 50mM phosphate buffer to obtain a concentration of 1mg / mL enzyme solution; then immerse the prepared nanoporous gold film in the enzyme solution to make Horseradish peroxidase is attached and fixed on the surface of nanoporous gold film, and the fixation time is 24 hours;

[0041] (3) Electrode preparation: paste the horseradish peroxidase / nanoporous gold complex prepared in step (2) on the surface of the glassy carbon electrode, and dry it in vacuum for 1 hour to prepare the horseradish peroxidase / nanoporous gold complex Gold / glassy carbon electrodes.

Embodiment 3

[0042] Example 3: Preparation of a nanoporous gold horseradish peroxidase sensor electrode

[0043] (1) Preparation of nanoporous gold: place an Au / Ag alloy sheet with a thickness of 100 nm in pure nitric acid, and corrode it for 10 minutes at a temperature of 30° C. to prepare a nanoporous gold film;

[0044] (2) enzyme immobilization: horseradish peroxidase is dissolved in pH7.0 concentration 50mM phosphate buffered saline, and the obtained concentration is 20mg / mL enzyme solution; Horseradish peroxidase is attached and fixed on the surface of the nanoporous gold film, and the fixation time is 6 hours;

[0045] (3) Electrode preparation: paste the horseradish peroxidase / nanoporous gold complex prepared in step (2) on the surface of the glassy carbon electrode, and dry it in vacuum for 2 hours to prepare the horseradish peroxidase / nanoporous gold complex Gold / glassy carbon electrodes.

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Abstract

The invention discloses a method for fast detecting aromatic pollutants in wastewater by means of a nano-porous gold horseradish peroxidase sensor. The method includes the steps that firstly, the sensor is prepared and represented; secondly, the sensor is calibrated or corrected; thirdly, aromatic pollutants in wastewater are detected. By means of the method, the problems that a traditional aromatic pollutant detection method is tedious in step and online detection is difficult can be effectively solved, the method has the advantages that the technology is simple and convenient, determination is accurate, specificity is high, response is fast, sensitivity is high, and operation is easy, various phenol and aromatic amine substance mixtures can be detected at the same time, and application prospects are broad.

Description

technical field [0001] The invention relates to a method for detecting aromatic pollutants in wastewater, in particular to a method for rapidly detecting aromatic pollutants in wastewater by using a nanoporous gold horseradish peroxidase sensor. It belongs to the field of electrochemical analysis and testing. Background technique [0002] In recent years, research on the monitoring and treatment of organic refractory pollutants has increasingly become a hot spot in the field of environmental monitoring. With the advancement of science and technology and the improvement of living standards, more and more pollutants are discharged into the environment from industrial and agricultural production and residents' lives. Among them, aromatic organic compounds are a kind of substances with strong stability, great harm and difficult biodegradation. Among them, the harm of phenolic and aromatic amine pollutants is the most serious. [0003] Phenolic substances often exist in wastewa...

Claims

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Application Information

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Patent Type & Authority Patents(China)
IPC IPC(8): G01N27/48G01N27/327
CPCG01N27/327G01N27/48
Inventor 王霞吴超
Owner SHANDONG UNIV
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