The invention discloses a semi-quantitative detection method for heavy
metal ions in a water-soluble sample based on a whole-
cell biosensor, and belongs to the technical field of environmental
biology. The method is convenient to operate, short in time consumption, low in cost, good in
visibility and easy to quantify. The SRRz
cracking gene is used as a report element, the response is quick, and the
optical density of the bacterial liquid is obviously reduced within 2 hours when the bacterial liquid is in contact with heavy
metal. According to the present invention, the SRRz splitting
gene is adopted as the report element so as to cause the splitting of the microbial thalli, the correlation between the obtained result and the Cd (II)
ion concentration is high, and the detection result can be determined by using naked eyes, and can be detected through a visible light spectrophotometer; x-gal is used for detecting the beta-galactosidase released outside the cells, and the operation is simple and convenient. The method is good in safety, free of
reagent addition, free of
pigment interference, short in time consumption, low in cost, specific, easy to realize high-
throughput screening and convenient to popularize; and
technical support can be provided for daily detection and high-
throughput screening of heavy
metal pollution.