33 results about "OD - Optical density" patented technology

The invention belongs to the technical field of biology, and particularly relates to milk-derived pentapeptide for promoting calcium mineral absorption and application of the milk-derived pentapeptide. The CPP containing the high-activity peptide fragment is obtained by optimizing a process, and the influence of the CPP on mineral absorption is verified by utilizing a zebra fish model. Then, the pentapeptide GPFPI with calcium absorption promoting activity is screened out from the CPP in combination with peptidomics and a molecular docking technology, functional verification is carried out, it is found that the pentapeptide presents a dose-dependent trend on Ca < 2 + > transport, the Ca < 2 + > transport amount is remarkably increased by 23.23% at 10 mu M, and meanwhile, zebra fish experiments show that intervention of the GPFPI effectively reverses accumulated optical density value reduction caused by dexamethasone, and the effect of inhibiting calcium absorption is achieved. Compared with a manufacturing module, the skull accumulative optical density value of a GPFPI (2-20 mu M) processing group is remarkably increased. It is shown that the synthetic peptide GPFPI can effectively promote Ca < 2 + > transfer and absorption, improve the osteoporosis symptom of zebra fish and increase deposition of calcium in bones.

The invention discloses a KLHL11 antibody high-precision detection method based on an enzyme-linked immunosorbent assay. The detection method comprises the following steps: preparing a microwell plate pre-coated with an anti-human KLHL11 antibody, dividing micropores into a plurality of detection groups, and adding KLHL11 protein or peptide fragment solutions with different concentrations, a to-be-detected solution with unknown concentration and a contrast solution into each detection group. The detection precision of a current detection instrument, the operation capability level of an operator and the reliability of the current detection instrument and the operation capability level are evaluated by executing enzyme-linked immunosorbent assay operation steps including sample filling, incubation, washing, color development and optical density measurement, obtaining the optical density value of a detection group and calculating the mean value, the standard deviation and the variation index of each group. By establishing a standard curve of optical density and concentration, the concentration of an unknown sample can be calculated, and the performance of an instrument in different concentration ranges can be verified. In addition, the problems of drifting and cross contamination of the instrument are further detected by setting a detection group with a relatively large time interval and a high-low concentration gradient experiment.

Disclosed is a method for gene expression by transient transformation of a wild rice seed using Agrobacterium. The method includes the following steps: subjecting a wild rice seed to induction culture and subculture in sequence, and selecting a resulting callus with a dense structure to allow pre-culture to obtain a pre-cultured callus; transforming a 1305Ubi-Ubi-GFP-H plasmid carrying a green fluorescent protein (GFP) gene into an Agrobacterium strain EHA105 or LBA4404 to obtain a positive Agrobacterium strain; subjecting the positive Agrobacterium strain to culture to obtain a bacterial suspension with an optical density (OD) value of 0.02 to 0.5 at a wavelength of 600 nm to obtain an infection bacterial solution; and infecting the pre-cultured callus with the infection bacterial solution to allow co-culture and recovery culture in sequence; observing an infection status of a recovered callus under a stereo fluorescence microscope, and calculating a transient expression rate.

The invention belongs to the technical field of biology, and particularly relates to casein source heptapeptide for promoting calcium absorption and utilization and application of the casein source heptapeptide. The CPP containing the high-activity peptide fragment is obtained through an optimization process, and the influence of the CPP on mineral absorption and conversion is evaluated by utilizing a mineral deficiency rat model; then, heptapeptide with calcium absorption promoting activity is screened out from the CPP in combination with peptiomics and molecular docking technologies, functional verification is carried out, it is found that when the VAPFPEV is 2 mu M, the Ca < 2 + > transport amount is remarkably increased by 25.25%, meanwhile, zebra fish experiments show that intervention of the VAPFPEV effectively reverses reduction of an accumulated optical density value caused by dexamethasone, and compared with a module making set, the VAPFPEV has the advantages that the VAPFPEV has the advantages that the VAPFPEV has the advantages that the VAPFPEV has the advantages that the VAPFPEV has the advantages that the VAPFPEV has the The skull accumulative optical density value of a VAPFPEV (1-20 [mu] M) processing group is obviously increased. It is shown that VAPFPEV can effectively promote Ca < 2 + > transfer and absorption, improve the osteoporosis symptom of zebra fish and increase deposition of calcium in bones.

The invention belongs to the technical field of sugar alcohol, and particularly relates to a method for quickly detecting bacteriophage in fermentation liquor, which comprises the following steps: centrifuging the fermentation liquor to obtain supernate, boiling the supernate in a boiling water bath for 2-5 minutes to obtain boiled liquor, visually comparing the turbidity difference between the supernate and the boiled liquor, and if the supernate is clear, judging whether the bacteriophage is in the boiling state or not. If the boiled liquid is turbid, the fermentation liquid is polluted by the bacteriophage; if the turbidity difference between the supernatant and the boiled liquid is not obvious, respectively measuring OD650 optical density values of the supernatant and the boiled liquid by using a spectrophotometer, comparing the optical density detection values, and if the difference between the two is not more than 0.5, indicating that the fermentation liquor is not infected by the bacteriophage; if the difference is greater than 0.5, the fermentation liquor is polluted by the bacteriophage. According to the method, whether the fermentation liquor is polluted by the bacteriophage or not is judged by visually comparing the turbidity difference before and after the supernate obtained after the fermentation liquor is centrifuged is heated and further verifying the difference of optical density values by using the spectrophotometer, and the method has the characteristics of rapidness, convenience and accuracy.

A diagnostic kit for a confirmatory assay using the indirect ELISA method that measures the levels of anti-Native Hapten antibodies produced during a real infection, thereby preventing large financial losses to livestock farm, by discerning “false positives” that present anti-LPS antibodies due to cross-reactions with enterobacteria and post-vaccinal antibodies for the diagnosis of bovine brucellosis in blood serum and individual milk (per animal) and bulk milk (tank), characterised by using the crude Native Hapten antigen, extracted from B. melitensis 16M strain, with no purification treatment and an effective adherence capacity, which is used to antigenize plates at a known concentration (1 μg per well), where using as reference positive and negative controls subjected to the lyophilisation (freeze drying) method to ensure their preservation, thereby avoiding the contamination and degradation of the antibodies present and ensuring the stability of the optical densities in said controls for a correct results interpretation of an indirect ELISA, are taken as reference. The lyophilisation method for controls that may be used in other diagnostic methods is also presented.

Provided herein are methods for the production of a vector with a size of at least 16 kb from bacterial cells comprising the consecutive steps of a) obtaining bacterial cells comprising a vector with a size of at least 16 kb, comprising an inducible origin of replication, b) inoculating culture medium with the bacterial cells comprising the vector, c) culturing the bacterial cells in the culture medium, d) adding one or more inducers of said inducible origin of replication to the culture medium when the bacterial culture has reached an optical density at 600 nm (OD600) of at least 20, e) further culturing the bacterial cells in the culture medium, f) optionally separating the bacterial cells from the culture medium, and g) recovering the plasmid from the bacterial cells. Also provided herein are vectors with a size of at least 16 kb comprising an inducible origin of replication for use in such methods.

The invention relates to the technical field of drug sensitivity evaluation, in particular to a riemerella anatipestifer drug sensitivity evaluation method which comprises the following steps: S1, bacterial liquid preparation: sampling a bacterial strain, inoculating the bacterial strain into a nutrient solution, and culturing to 108CFU / mL bacterial concentration; s2, automatic drug dilution and addition: a system automatically dilutes to-be-tested drugs to different concentrations according to experimental design, and the to-be-tested drugs are added into the bacterial liquid; s3, real-time optical detection: collecting optical density data once every 30 minutes by an optical detection module, and recording growth changes of the bacterial liquid under different drug concentrations; s4, data analysis and output: the system calculates the optical density data, draws a bacterium growth curve and calculates the minimum inhibitory concentration, according to the scheme, a micro liquid dilution method and an automatic drug sensitivity evaluation system are adopted, drug sensitivity data can be obtained within 6-8 hours, the detection time is greatly shortened, and the detection efficiency is improved. Automatic data processing: through optical density detection, image processing and data analysis technologies, the inhibition effect of drugs on bacteria is monitored in real time, and data is more objective and accurate.

The invention relates to an adenovirus detection test strip based on high-concentration colloidal gold and a preparation method thereof, and belongs to the technical field of adenovirus detection. The adenovirus detection test strip is composed of a sample pad, a combination pad, a nitrocellulose membrane, a water absorption pad and a bottom plate, the combination pad is loaded with an adenovirus detection antibody marked by high-concentration colloidal gold, the nitrocellulose membrane is provided with a detection line, the detection line is loaded with an adenovirus capture antibody, the particle size of the high-concentration colloidal gold is 60 nm, and the particle size of the high-concentration colloidal gold is 20 nm. And the optical density value is 100. According to the invention, the high-concentration colloidal gold is used for marking the adenovirus antibody, and an optimized marking process is combined, so that the binding efficiency of the colloidal gold and the antibody is enhanced, the detection sensitivity is improved, the problem of low sensitivity of adenovirus detection by using an immunochromatography technology in the prior art is solved, and the method has popularization value.

The invention discloses a high-density lipoprotein subtyping detection method, a detection kit as well as a preparation method and application of the detection kit. According to the method, high-density lipoprotein in serum or plasma is separated by adopting a polyacrylamide gel electrophoresis method; after high-density lipoprotein in serum or plasma is dyed with a dyeing solution, a mixed solution is taken and added to a gel column; under the action of an electric field, the high-density lipoprotein particles are sequentially separated according to the sizes of the high-density lipoprotein particles through a polyacrylamide gel molecular sieve effect; a protein electrophoretic separation result passes through a scanning system of the electrophoresis device to obtain an optical density map of a high-density lipoprotein electrophoretic band; converting the optical density spectrum into a peak pattern spectrum, and performing area proportion analysis; and obtaining the accurate content of each component of the high-density lipoprotein subtype according to the total high-density lipoprotein content and the proportion of each peak pattern area. Classification is carried out according to the particle size of the high-density lipoprotein, the high-density lipoprotein is subtyped and quantitatively detected, and the detection sensitivity is improved.

Accurate hydration measurement is crucial for health. This patent presents a non-invasive method for quantifying hydration in tissues and bloodstream using heartbeat-based optical density (OD) analysis. By employing LEDs at specific wavelengths for water and blood OD measurements and correcting for melanin interference, this method calculates hydration as the ratio of water OD to blood OD. The technique offers precise, rapid, and minimally invasive hydration assessment, addressing traditional method limitations. This approach is suitable for wearable or stand-alone devices, enhancing health monitoring and personalized hydration strategies.

The invention provides a method for screening an early stage of coronary heart disease N1 protein by using a kit, and belongs to the technical field of biomedical detection, and the method comprises the following steps: sorting mononuclear cells; performing incubation; collecting a single cell fluorescence image; performing algorithm analysis; a single cell imaging and AI quantitative strategy is adopted, target mononuclear cells are enriched through high-purity sorting, target protein is labeled with a high-affinity antibody, single cell fluorescence distribution is captured through high-resolution microscopic imaging, integral optical density is calculated through an AI algorithm, and subcellular level spatial quantification of protein expression is achieved. Biomarker detection is improved from a population mean value to a single cell resolution ratio, and the analysis capability on trace expression is remarkably improved.

The utility model discloses an intelligent bioreactor integrated with a multi-parameter biochemical analysis function. The intelligent bioreactor comprises an optical detection module, an electrochemical sensor module and an overflow pool biochemical detection module, the optical detection module adopts a transmission-type optical measurement principle and is used for measuring the optical density of the culture solution; the electrochemical sensor module adopts an electrochemical principle and is used for detecting the concentration of at least 10 metabolites in the culture solution; the overflow pool biochemical detection module adopts an ion selective electrode principle and is used for simultaneously detecting the concentrations and pH values of NH4 < + >, K < + >, Na < + > and Ca < 2 + > ions in the culture solution; the three modules are sequentially connected through fluid channels to form a complete detection flow path.

A transient transformation method for wild rice, Zizania latifolia, comprising: subjecting a wild rice seed to induction culture and subculture in sequence, and selecting a resulting callus to obtain

The invention belongs to the technical field of bacterial activity detection, and particularly relates to a method and a device for rapidly detecting the microbial activity of water body bacteria based on a dual-wavelength transmission spectrum, the method particularly focuses on the transmission spectrum characteristics of an escherichia coli liquid under the wavelength of 230nm and 670nm, and a relationship model between the dual-wavelength optical density ratio and the bacterial activity is constructed to determine the microbial activity of the water body bacteria. The rapid and accurate detection on the bacterial activity is realized. Compared with a traditional detection method, the method has the advantages that the operation process is simplified, the detection time is shortened, the detection sensitivity and accuracy are remarkably improved, and the actual activity level of bacteria can be reflected more accurately. In addition, the invention also provides a rapid detection device which is simple and convenient to operate and wide in application range, provides efficient and reliable technical support for detecting the activity of the bacterial microorganisms in the water body in the fields of environmental monitoring, food sanitation and the like, and has important practical application value.

This patent describes an advanced method for the accurate optical quantification of antioxidant carotenoids in biological tissues. The method addresses interference from melanin and hemoglobin through comprehensive correction techniques. It involves creating a tangent line for melanin correction using absorbance values at 650 nm and 700 nm, measuring the corrected optical density (OD) of carotenoids at 488 nm, measuring the corrected OD of blood at 577 nm, converting the Blood OD from 577 nm to 488 nm using oxyhemoglobin and deoxyhemoglobin extinction coefficients, and subtracting the Blood OD from the Carotenoid OD to obtain the final Carotenoid OD. This innovative approach provides reliable, non-invasive, and precise measurements of carotenoid levels in tissues, facilitating improved health assessments and personalized nutrition strategies.

The invention relates to the technical field of data processing, and discloses a combined drug sensitive test data processing method, device and equipment and a storage medium, and the method comprises the following steps: obtaining a combined drug sensitive plate optical density value matrix output by a microplate reader and a set growth inhibition threshold; based on the optical density value matrix of the combined drug sensitive plate and the growth inhibition threshold, respectively judging the growth state corresponding to each hole in the combined drug sensitive plate; calculating a corresponding partial bacteriostatic concentration index according to the hole with the growth state in growth inhibition; and determining the combined effect of the two drugs based on the calculated minimum partial bacteriostatic concentration index. According to the invention, the accuracy and reliability of the combined drug sensitive test result can be improved.

A computer system is provided for determining the relative effectiveness of anti-cancer drugs. The interface has selectable options, including an option to manage drug testing parameters, and enables user selection of desired drug testing parameters in relation to a virtual well plate associated with a physical well plate of a spectrophotometer. The computer system causes the spectrophotometer to start a drug test, wherein the physical well plate includes at least one test well containing viable cancer cells; and at least one drug candidate in a predetermined concentration; and at least one control well containing the viable cancer cells alone. The system records the optical density of the well at a predetermined wavelength at selected time intervals for a selected duration of time, and stores the optical density and time measurements in the database. An activity value is calculated from the optical density and time measurements, and a correlation is displayed between the activity value and the drug candidate's ability to induce apoptosis in the cancer cells.

The invention provides a breast cancer auxiliary diagnosis method and system based on virtual staining, and the method comprises the steps: firstly obtaining an HE staining section, carrying out the enhancement preprocessing, and generating an ER / PR / HER2 virtual IHC image through a GAN-based virtual staining model; on the basis of a DAB channel obtained through color deconvolution integral separation and cancerization areas and cell nucleus instance segmentation results obtained on the virtual IHC and / or HE images, indexes such as nucleus density, nucleus morphology, ER / PR positive cell nucleus proportion, HER2 membrane continuity / coverage rate, positive area proportion and average optical density are calculated, and a superposition and structuring report is generated. According to the method, combined losses such as positive region consistency, membrane ring continuity, nuclear grade consistency and grading alignment are introduced in the training stage, a nuclear density self-adaptive activation function is adopted, and the consistency and robustness of virtual dyeing and quantitative evaluation are improved. IHC reagents can be reduced, the period is shortened, and the cross-section comparison difficulty is reduced.

The embodiment discloses an optical density measurement and testing including a purification device that separates live cancer cells from dead and non-cancer cells of a microbiology sample. A drug addition device and a drug dosage sequencer introduce controlled dosages of at least one drug treatment into the live cancer cells. An optical density measurement device with an optical sensor captures high-resolution images of the live cells at predetermined intervals, and an optical spectrophotometric reader quantifies cell populations following treatment. A flow cytometry device measures fluorescent intensities of immune checkpoint markers and tumor antigens to generate immune system activation profiles. A processor subsystem analyzes cell death rates and immune responses to create integrated drug response profiles, and a computer application compares these results with patient-specific genetic and clinical data to produce personalized treatment recommendations.

The invention relates to the technical field of detection, and particularly discloses a small molecule compound detection method and application thereof. The small molecule compound detection method comprises the following steps: (1) enriching and extracting to-be-detected small molecule compounds in a to-be-detected sample to obtain a to-be-detected solution; (2) obtaining a magnetic nano enzyme coated with an antibody; (3) mixing the two solutions, performing magnetic separation, and redissolving to obtain a to-be-detected particle solution; (4) in the MetaSPR chip detection hole coated with the to-be-detected small molecule compound hapten, detecting the optical density of the blank detection liquid and the optical density of the to-be-detected particle solution after incubation, and calculating the concentration value I of the to-be-detected small molecule compound according to the optical density difference between the blank detection liquid and the to-be-detected particle solution; and (5) calculating the solution of which the end point optical density is detected through a catalytic colorimetric method to obtain a concentration numerical value II of the to-be-detected small molecule compound. The dual-mode detection method disclosed by the invention is simple and convenient to operate, high in accuracy, low in detection limit, stable in performance, high in detection flux and suitable for safe and rapid screening of food.

The invention relates to the field of bioengineering, and discloses a process for producing 3 '-sialic acid lactose by fermentation, which comprises the following steps: solution preparation, strain recovery, fermentation initial culture, first induction, multi-stage material supplementation, second induction and fermentation later control. The method comprises the following steps: optimizing a culture medium formula (taking cane sugar and phosphate as main materials and avoiding a high-cost mixed carbon source), performing staged induction conditions (taking an optical density (OD) value of fermentation liquor as a trigger signal) and accurately controlling a material supplementing speed; according to the method, the final yield of 3 '-SL is remarkably increased and reaches 42-45g per liter of fermentation liquor, and the content of byproducts such as lactose and sialic acid is effectively reduced to be within 1%-2%. Compared with the prior art, by optimizing the metabolism flow direction of the strain and simplifying the fermentation process flow, the production cost is remarkably reduced, meanwhile, the fermentation period is shortened, and the problems of low fermentation efficiency, many byproducts and high process complexity in the prior art are solved.

The invention provides an application of SERPINB10 protein as a diagnostic marker in preparation of a diagnostic kit for identifying eosinophilic esophagitis and reflux esophagitis. The invention also provides an application of the SERPINB10 protein in preparation of a product for differential diagnosis of eosinophilic esophagitis and reflux esophagitis. The invention also provides application of the reagent for detecting the SERPINB10 protein in preparation of a diagnostic kit for identifying eosinophilic esophagitis and reflux esophagitis. When the SERPINB10 protein immunohistochemical detection integral optical density of the esophageal tissue sample of the subject is greater than or equal to 41666, it is shown that the patient suffers from eosinophilic esophagitis, and otherwise, the patient suffers from reflux esophagitis. The invention establishes an EoE diagnosis and differential diagnosis system which is objective, accurate and low in missed diagnosis rate.

The embodiments disclose an optical density measurement and testing system including a purification device configured to detect and isolate live cancer cells of a patient microbiology sample, a drug addition device configured to infuse a drug treatment to the live cancer cells, an optical density device configured to capture images of the microbiology sample, an optical microplate spectrophotometric reader coupled to the optical density device configured to measure the density of living cells after infusion of the at least one drug treatment, a computer coupled to the optical microplate spectrophotometric reader configured to determine measured rates of death over a period of time based on the interval population growth data, and an optical density application operating on the computer configured to use the measured rates of death and known predetermined genetic markers, resistances and allergies of the patient associated with the drug to generate clinician treatment recommendations for the patient.

The present invention relates to the technical field of magnetic microparticle chemiluminescence immunoassay, and specifically discloses a method for determining the magnetic loss rate of magnetic beads. The determination method comprises the following steps: constructing a standard solution with a magnetic bead concentration gradient; determining the optical density of the standard solution at each magnetic bead concentration, and drawing a magnetic bead concentration-optical density standard curve; performing magnetic separation and washing on the sample to be tested, and determining the optical density value of the sample to be tested; substituting the optical density value of the sample to be tested into the magnetic bead concentration-optical density standard curve to obtain the magnetic bead concentration of the sample to be tested, and calculating the magnetic bead amount of the sample to be tested from the magnetic bead concentration of the sample to be tested; comparing the magnetic bead amount of the sample to be tested with the total amount of magnetic beads added to the sample to be tested before magnetic separation and washing to obtain the magnetic loss rate; wherein the type of magnetic beads contained in the sample to be tested is the same as the type of magnetic beads in the magnetic bead concentration-optical density standard curve. The determination method of the present invention has the characteristics of simple testing, high efficiency and low cost.

The present invention provides an optical density based non-invasive, field usable method for the detection of pregnancy in healthy ruminant livestock using naturally micturated urine. The present invention also provides a colorimetric kit for detection of pregnancy in ruminants livestock. The test also helps in differentiating between pregnant and non-pregnant ruminant females using urine samples.

A method for predicting the efficacy of antibody drug conjugate (ADC) therapy involves determining the average optical density (OD) of the membrane and, optionally, the cytoplasmic staining of each cancer cell in a whole slide image. The diagnostic antibody to which the dye is linked targets the folate receptor alpha protein in the same way as the ADC antibody. Statistical calculations are performed on the OD of the staining, such as calculating the median, absolute deviation, the difference in OD between membrane and cytoplasmic staining, the percentage of cells with the lowest OD, or the sum of minimally stained cells and poorly stained cells near the stained cells. For example, based on whether the median OD is below, between, or above two OD thresholds, a zero, high, or low ADC dose is recommended, respectively. The upper and lower OD thresholds are correlated with the response of training patients who were treated with the ADC and whose stained cancer tissues were analyzed.

A liquid sporulation method is described. A first liquid culture is prepared by adding bacterial cells to a sporulation broth, wherein an optical density (OD600) of the first liquid culture is in a range of 0.001 to 0.01. The first liquid culture is incubated and an optical density (OD600) thereof is increased to be in a range of 0.2 to 2.0. Second liquid culture is prepared by adding the incubated first liquid culture to a predetermined amount of additional sporulation broth, wherein an optical density (OD600) of the second liquid culture is in the range of 0.001 to 0.1, and a ratio of a volume of the second liquid culture to a volume of the first liquid culture is in a range of 10:1 to 150:1. The second liquid culture is incubated so an optical density (OD600) thereof is increased to be in a range of 1.0 to 4.0.

The present application discloses a drug sensitivity result recognition method, device, electronic device and readable storage medium. Among them, the method includes obtaining the optical density data of the target strain at the target concentration of the drug within the first time period as the data to be tested. According to the optical density data of the target strain at different concentrations of the target drug and without adding the target drug, the growth information of the target strain in the future preset time period is determined to obtain the extended test data. According to the concentration information characteristics of the extracted extended test data and the similarity information characteristics between the optical density data of the target strain at the multiple reference concentrations of the target drug and without adding the target drug respectively, the growth characteristics to be recognized are generated. The growth characteristics to be recognized are input into the pre-trained growth prediction model to obtain the predicted value of the growth situation of the target strain in the target drug, so as to realize the efficient, low-cost and accurate recognition of the drug sensitivity result.

The invention discloses a method for determining the dilution ratio of a biochemical super-linear sample based on reaction curve characteristics. The method comprises the following steps: after a biochemical reaction begins, according to a point P corresponding to a first optical density value exceeding 2.0000 displayed by a biochemical analyzer, determining the optimal dilution ratio N of the biochemical super-linear sample according to the following correspondence: the point P is 19-24, and the N is 2; the P point is 17-18, and the N is 3; the P point is 15-16, and the N is 4; the P point is 14, and the N is 5; the P point is 13, and the N is 8; the P point is 12, and the N is 25; wherein the P point is a reading point for the biochemical analyzer to read the OD value every 18 seconds. The method can quickly and automatically calculate the optimal dilution ratio required for generating the super-linear sample in the first detection, so that the number of times of repeated detection is remarkably reduced, the working efficiency is improved, and precious reagents and samples are saved.