39 results about "OD - Optical density" patented technology

A microscope array with staggered rows of magnifying imaging systems is used to scan a biological tissue sample in a single linear pass to produce an image and corresponding optical-density data. A conventional computerized algorithm is used to identify, isolate and produce segmented images of nuclei contained in the image. The OD values corresponding to nuclear chromatin are used to identify numerical patterns known to have statistical significance in relation to the health condition of the biological tissue. These patterns are analyzed to detect pre-neoplastic changes in histologically normal-appearing tissue that suggest a risk for the development of a pre-malignant and a potentially malignant lesion. This information is then converted to a visually perceptible form incorporated into the image of the tissue sample and is displayed for qualitative analysis by a pathologist.

The invention relates to an immunochromatography quantitative detection instrument and a detection method of colloidal gold, latex and the like for quantitative detection of an immunochromatography test strip. A to-be-detected sample is dripped onto a transverse immunochromatography test strip which is marked in advance to coat a corresponding antigen-antibody, then the test strip is inserted into a detection tank of the immunochromatography quantitative detection instrument which is used for confirming the color chromaticity and background chromaticity of C and T line regional positions in a nitrocellulose membrane of the test strip by virtue of multi-wavelength scanning, identification computational analysis is performed on optical density values of a quality control band and a detection band according to a built-in algorithm of the instrument, then the concentration of positive markers contained in the to-be-detected sample is obtained, a report is printed, and a result value is stored. By using the immunochromatography quantitative detection instrument and the detection method, the influence of the difference among different immunochromatography test strips on detection results can be avoided, and precise and stable quantitative detection is really realized. In addition, the immunochromatography quantitative detection instrument and the detection method have the advantages of good open generality and are suitable for determining the detection results of immune test paper with different specifications and different detection types.

The invention discloses a method for detecting DNA content of tumor cells based on image analysis. In this method, a camera installed on the upper part of the microscope collects sliced ​​images observed under the microscope and transmits them to a computer, and the images are analyzed and measured by analysis software. The process is: collect the scale image and calibrate the length; collect the background image and measure the pixel gray value of the background image; collect the normal diploid cell image, perform image segmentation on the diploid cell image, and measure the gray value of the diploid cell nucleus. Calculate the average optical density value of the diploid cell nucleus; collect tumor cell images, segment the tumor cell image, measure the optical density value of each tumor cell nucleus pixel, and calculate the DI value and area of ​​each tumor cell nucleus; generate DNA histogram and scatter diagram, count the number of various cells according to DI classification, and generate a DNA test report. The method has the advantages of low cost, fast measurement, reduction of artificial judgment errors, easy preservation of pictures and the like.

The operator downloads, from a data file into the random access memory of a personal computer, integrated optical density and profile area data for a plurality of nuclei and partial nuclei in a Feulgen-stained histologic tissue section; the thickness of the section; and the integrated optical density of an intact diploid nucleus. The operator then operates a computer program which plots the data curve together with a reference line and a reference curve. The data curve is scaled and the reference curve is recalculated and redrawn through a number of iterations until the operator is satisfied that the scaled data curve terminates on the reference line, and the scaled data curve is as nearly congruent as possible with the latest reference curve. The program calculates and displays the corrected DNA index on a video screen, the contents of which the operator may print on a printer.

Integrated apparatus and method for hematological analyses, wherein the apparatus comprises, arranged substantially in line and integrated substantially in a single machine, a device (14) of the optical type to detect substantially instantaneously the speed of blood sedimentation (ESR) by measuring the optical density, or absorbance, of the blood sample, and a measuring assembly (18) with a cell-counter function or suchlike.

The invention relates to an analysis method for plankton type community DNA dactylogram in urban sewage, the method is as follows: metagenome of plankton type community in urban sewage is amplified through using a primer aiming at 16S rDNA and 18S rDNA, and undergoes denaturing gradient gel electrophoresis to obtain a fingerprinting of community DNA. Firstly, the metagenome DNA of the plankton type community is extracted; secondly, the object gershgorim band of the metagenome is amplified; thirdly, the DGGE electrophoretic separation is performed to obtain the fingerprinting of the plankton type community DNA; fourthly, a DGGE gel picture is analyzed through using DNA UVI software, using the optical density value of the gershgorim band to indicate the gershgorim band; and finally, data analysis is performed through using statistics software. The analysis method for plankton type community DNA dactylogram in urban sewage has the advantages of convenience and efficiency, excellent repeatability, high sensitivity and low cost, capability of being applied to biological monitoring of urban sewage.

The invention discloses a method for monitoring oxygen supply parameters.The optical density (OD) and optical density optical density changing quantity (delta OD) of blood in adjacent descending aortas are detected through an Hb-SO2 sensor arranged in an esophagus via the esophagus wall, and the hemoglobin concentration of blood is obtained through corresponding calculation.The invention further discloses an adopted detecting device.The hemoglobin concentration and even oxygen supply of a patient in the perioperative period can be subjected to routine monitoring like monitoring of SpO2, the oxygen supply of the patient can be kept within a safe range to avoid anoxia injuries and greatly improve the safety of anesthesia and an operation, and meanwhile the effort saving functions of reducing the use frequency of complex invasive monitoring and reducing the work intensity of an anesthetist are achieved.

The invention discloses an acetic acid and xylose co-used Kluyveromyces marxianus strain with a biological preservation number of CGMCC No.16757. The strain is obtained by long-term orient domestication of Kluyveromyces marxianus through cellulosic hydrolysate and is prepared by the following steps: picking single yeast colonies from a YPD flat plate and carrying out 40 DEG C overnight culture; reinoculating collected cells to a fresh culture medium containing the cellulosic hydrolysate with certain concentration, enabling an optical density value of the culture medium at 620nm to be approximate to 0.3; culturing for 24 to 48 hours at 40 DEG C and 150rmp / min; when OD620 of the acetic acid and xylose co-used Kluyveromyces marxianus strain is greater than or equal to 2.0 (5 to 7 generations), collecting the cells; repeating the steps until the growth speed of the cells is obviously improved under the stress condition of an inhibitor with the concentration; improving the concentration ofthe cellulosic hydrolysate and starting new repeated culture; after continuous domestication, coating the YPD flat plate containing the cellulosic hydrolysate with the collected cells and culturing at40 DEG C for 24 to 48 hours; picking the single colonies with good growth situation and putting into a YPD liquid medium, and culturing at 40 DEG C and 150rmp / min for 24 to 48 hours; preserving dominant resistance clones in 30 percent glycerinum.

A method for producing a strain of Saccharomyces cerevisiae with introduced genes coding for xylose reductase, xylitol dehydrogenase and xylulokinase and with improved ethanol production, improved xylose conversion, reduced xylitol production and improved inhibitor tolerance is described. The method comprises culturing a strain of Saccharomyces cerevisiae at a continuous mode with a medium comprising essentially only xylose as carbon source at a temperature of 25-38° C., preferably 30-35° C., and an airflow of 0.040-0.055 vvm, and increasing the dilution rate to maintain a constant cell level, said cell level being in the range of 1.5-3.0 determined by optical density or equivalent analytical means, and adding at least one inhibitor to the cells and gradually increasing the addition of said inhibitor. Further, strains of Saccharomyces cerevisiae obtained by the method according to the invention are described.

The invention discloses a growth effect and acute and chronic toxicity detection method of luminous bacteria. The method comprises the steps of: preparing an experiment sample, a negative control sample and a positive control sample; preparing a luminous bacteria solution; adding the luminous bacteria solution into a 96-pore cell culture board, then placing the 96-pore cell culture board on a microplate reader, and measuring an initial optical density value and an initial bioluminescence value 30 minutes later; adding the experiment sample, the negative control sample and the positive controlsample into the luminous bacteria solution, measuring the optical density value and the bioluminescence value every 30 minutes within the first 1 hour, and then measuring the optical density value and the bioluminescence value every 1h, and performing the test for not less than 24h; obtaining an optical density mutation time and a bioluminescence mutation time; and calculating an acute luminescence inhibition rate, a chronic luminescence inhibition rate and a growth inhibition rate. The growth effect and acute and chronic toxicity detection method disclosed by the invention can be used for simultaneously evaluating the growth effect, and acute and chronic toxic effects of a test substance on the luminous bacteria, the accuracy is high, high throughput of the toxicity test is achieved, andthe test efficiency is high.

The invention relates to the technique of development and application of spirulina and aims at providing a method for detecting production trait goodness and badness of spirulina strains. According to the method, a Tris-HC1 extracting solution is utilized for extraction so as to obtain water-soluble protein of spirulina cells; protein samples are separated by using sodium dodecyl sulfate-polyacrylamide gel electrophoresis, subsequently the optical density value of a protein zone at 102kD of a protein electrophoresis diagram is detected, and a dendrogram of the spirulina plants is established according to the value; if the optical density value of a candidate strain at the protein zone at the 102kD is larger than 140 and the candidate strain is gathered together with a known stain with good production nature, the stain is good in production nature and is applicable to large-scale cultivation and production; and if the optical intensity value of the protein zone at 102kD is less than 40 and the candidate strain is gathered together with known strains with poor production nature, the stain is poor in production nature and is not applicable to large-scale cultivation and production. The method is simple, efficient, reliable and low in cost, and complex tests and analysis on nucleic acid sequencing and bioinformatics comparison do not need to be carried out, therefore, the method is applicable to large-scale and high flux separation.

The invention relates to a kit for rapid quantitative detection of swine fever antibody. The kit includes a colloidal gold immunochromatography test paper card and a standard curve information card. The colloidal gold immunochromatography test paper card is provided with colloidal gold-labeled hog fever E2-E0 The fusion expression antigen, the colloidal gold-labeled classical swine fever E2-E0 fusion expression antigen is synthesized by connecting the classical swine fever E2-E0 fusion expression antigen and a certain amount of colloidal gold particle solution through physical adsorption, and the colloidal gold particle solution contains The average particle size of colloidal gold is 30-35nm. After the sample to be tested is added to the colloidal gold immunochromatography test paper card, use a color analyzer to read the optical density values ​​of the detection zone and the accusation zone of the test paper card, and then calculate through software according to the standard curve recorded on the standard curve information card. The exact content of swine fever antibody. The invention is easy to operate and produces results quickly, and is a very convenient and good tool for accurately evaluating the immune status of swine fever.

The invention relates to a method for separating, from a culture medium, an enzymatic cocktail and the fungus Trichoderma reesei, the culture medium resulting from enzyme production by the fungus, method in which: said culture medium is subjected, in a period of no longer than 30 h from the halting of production, to a separation on a filter press lined with a fabric having a porosity of 3-20 μm, so as to obtain a filtrate having a corrected optical density OD at 600 nm of less than 2.5; and the liquid phase obtained is subjected to tangenital micro-filtration on a ceramic membrane having a cut-off limit of 0.5 to 1.4 μm, so that the corrected OD at 600 nm does not exceed 0.1.

The invention provides a method for testing bacterial drug sensitivity by TTC (2,3,5-triphenyte-trazoliumchloride) reduction reaction. The method comprises the steps as follows: preparation of an antibiotic solution; preparation of a 96-well plate with gradient antibiotic concentration; preparation of a bacterial suspension; constant temperature culture; result interpretation: if a medium is red after color reaction, the tested bacteria can tolerate antibiotics at the concentration, drug sensitivity test results are judged sensitively and accurately, the minimum inhibitory concentration is obtained, and thus, errors caused by visual judgment of operators and errors produced by turbidity of the medium when an instrument reads optical density value of a culture in plate wells are avoided. Then, in combination with CLSI (the Clinical Laboratory Standard Institute) identification standards, the sensitivity results of the tested bacteria to various antibiotics are interpreted to provide a guidance basis for selection of reasonable antibiotic treatment, and thus, accurate evaluation results are provided for antibacterial spectra and antibacterial activity of new drugs, bacterial drug resistance is better monitored, the epidemic trend of drug-resistant bacteria is grasped, and food and public health safety are ensured.

The invention discloses an SPR-SERS dual-mode sensor for detecting a disease nucleic acid marker as well as a preparation method and the application of the SPR-SERS dual-mode sensor. According to the SPR-SERS dual-mode sensor, an SPR sensing mode is realized by extracting integral optical density of dark field color in a dark field microscopic image, and a ratio type SERS sensing mode is realized by acquiring an intensity ratio of SERS characteristic peaks of ROX molecules and 4-mercaptobenzoic acid (4-MBA) molecules. For miRNA-652 detection, nucleic acid single chains C, H1 and H2 in the first reagent, the second reagent and the third reagent are DNA chains which are designed for miRNA-652 and have different nucleotide sequences. The SPR-SERS dual-mode sensor disclosed by the invention is simple to prepare, high in detection sensitivity and good in reliability, the detection limits of an SPR sensing mode and an SERS sensing mode on nucleic acid detection in serum can reach the level of flying mole per liter, and nucleic acid markers related to diseases can be detected in complex biological samples.

The invention relates to a method and a system for performing DNA ploidy quantitative analysis on a cell image based on a Pasteur staining mode. A cell slide obtained by staining in the Pasteur staining mode is scanned, a single cell in the obtained cell image is processed by adopting a set integral optical density analysis model to obtain an integral optical density value of the single cell, after all the single cells are detected by adopting a set cell detection model, part of the single cells are selected from all the single cells which are detected to be normal to serve as sample cells, the counted integral optical density mean value of the sample cells serves as a control value, and a DNA quantitative analysis result of the cell slide is obtained according to the calculated DNA indexes of all the cells in the cell slide, wherein the analysis model adopts a regression neural network of an attention mechanism and a feature pyramid mechanism, and is obtained by training by taking an integral optical density value under Foer root staining corresponding to a single cell under Pasteur staining as a regression target. According to the invention, the accuracy of quantitative analysis of the DNA ploidy is ensured.

The invention relates to a method for detecting pig fat deposition by using phylaxin expression level, which comprises the following steps: 1) extracting total DNA from detected pig tissue, carrying out inverse transcription to obtain a cDNA sample; 2) carrying out PCR amplification on the cDNA sample from the step 1) by primer pair shown in SEQ ID NO:2 and 3 to obtain a gene fragment shown in SEQ ID NO:1; 3) carrying out PCR amplification on the housekeeping gene by primer pair shown in SEQ ID NO:5 and 6 to obtain a gene fragment shown in SEQ ID NO:4; 4) carrying out agarose gel electrophoresis on the PCR products from the step 2) and step 3), comparing the optical density value in a gel imaging system. According to the invention, the fat pigs and lean type pigs with 1 month age, 3 months age, 5 months age and 7 months age can be taken as detection objects, the expression level of fat cytokine phylaxin (resistin) can be detected, the protein level of resistin is taken for reflecting the deposition condition of the pig fat. The method of the invention has the advantages of rapidity, convenience, accuracy and no influence on normal growth of pigs, and is suitable for dynamically monitoring the fat deposition condition of pigs in the culture process.

The invention discloses a diagnostic kit and an application of MAK16 in preparation of an early diagnostic reagent against systemic lupus erythematosus (SLE). The diagnostic kit can be used for earlydiagnosis of systemic lupus erythematosus disease. The kit includes an enzyme-labeling plate, human protein MAK16 as an antigen, a coating buffer, standard serum, an enzyme-labeling reagent, a confining liquid, and a sample diluent. By coating the human protein MAK16 on the enzyme-labeling plate, optical density of a IgG antibody of the anti-human protein MAK16 in serum is tested to assist diagnosis of systemic lupus erythematosus. The invention provides the sensitive, safe, reliable, and easy-to-operate commercial kit, helpful for auxiliary diagnosis and differential diagnosis of SLE by qualitatively detecting the level of the IgG antibody of the anti-human protein MAK16 in the human serum. The kit provided by the invention is used for screening or initial diagnosis of SLE disease with 88.12% of specificity and 48.98% of sensitivity, and used for differential diagnosis of SLE disease with 86.50% of specificity and 39.50% of sensitivity. The kit can be used as a helpful supplement to existing SLE diagnostic markers.

Provided herein is a method for producing a vector of at least 16 kb in size from a bacterial cell, comprising the following consecutive steps: a) obtaining a bacterial cell comprising a vector of at least 16 kb in size, said vector comprising an inducible origin of replication, b) inoculating a culture medium with the bacterial cell comprising the vector, c) culturing the bacterial cell in the culture medium, the present invention relates to a method for producing plasmids from bacterial cells, comprising the steps of: a) adding one or more inducers of an inducible starting point of replication to a culture medium, d) adding one or more inducers of the inducible starting point of replication to the culture medium when the optical density (OD600) of the bacterial culture at 600 nm reaches at least 20, e) further culturing the bacterial cells in the culture medium, f) optionally isolating the bacterial cells from the culture medium, and g) recovering plasmids from the bacterial cells. Also provided herein are vectors having a size of at least 16 kb comprising an inducible origin of replication for use in such methods.

The embodiment of the invention relates to the technical field of spectrum detection, and discloses a precise RNA concentration detection method and device, equipment, a medium and an application. The method comprises the steps: receiving total optical densities of collected pollutants at wavelengths of 260 nm, 280 nm and 320 nm; deducting a background reference in the total optical densities to obtain a basic total optical density, and decomposing the basic total optical density; rewriting the formula according to the proportional constants [delta]OD280RNA% and [delta]OD260PRO%, and obtaining the [delta]OD260RNA and the [delta]OD280PRO; and calculating the concentration M of RNA and the concentration N of protein in the pollutants according to the [delta]OD260RNA, the [delta]OD280PRO and the [delta]OD260PRO%. By implementing the embodiment of the invention, the comprehensive spectrum generated by the RNA and the protein in the same pollutant is split, so that the aim of simultaneously detecting the RNA and the protein is fulfilled.

The embodiment of the invention relates to the technical field of spectrum detection, and discloses an accurate DNA concentration detection method, device and equipment, a medium and application. The method comprises the following steps: receiving the total optical densities of collected pollutants at wavelengths of 260 nm, 280 nm and 320 nm; deducting a background reference in the total optical density to obtain a basic total optical density, and decomposing the basic total optical density; re-writting the formula according to the proportional constant delta OD280DNA% and the proportional constant delta OD260PRO% to obtain thedelta OD260DNA and delta OD280PRO; and calculating the concentration M of the DNA and the concentration N of the protein in the pollutants according to the delta OD260DNA, the delta OD280PRO and the delta OD260PRO%. By implementing the embodiment of the invention, the comprehensive spectrum generated by DNA and protein in the same pollutant is split, so that the purpose of simultaneously detecting DNA and protein is achieved.

The invention discloses a method for detecting the loading capacity of streptavidin magnetic microspheres combined with free biotin. The method comprises the steps of pretreating streptavidin magneticnano-microsphere solutions, respectively adding the pretreated streptavidin magnetic nano-microsphere solutions into a plurality of groups of biotin solutions for affinity adsorption to form a relatively stable complex, and cleaning the plurality of groups of complex products with a cleaning solution; adding horse radish peroxides (Biotin-HRP) labeled by biotin for adsorption; adding a color developing solution into the marked product to carry out a color developing reaction; measuring the OD (optical density) value of the solution after the chromogenic reaction; and calculating according tothe obtained multiple groups of OD values to obtain the loading capacity of streptavidin magnetic nano-microspheres combined free biotin. According to the detecting method, the loading capacity of streptavidin combined with free biotin can be detected more accurately and quickly, a basis is provided for the use amount of downstream streptavidin magnetic nano-microspheres, and the method is easy and convenient to operate, low in cost and high in sensitivity.

The invention relates to a technology of development and utilization of botryococcus, and aims at providing a sample pretreatment and determination method for detecting optical density values of the botryococcus. The method comprises putting to-be-detected botryococcus into a centrifuge tube, adding particle-type Xanthomonas polysaccharide, putting in an ice-water bath, and treating with ultrasonic to make botryococcus colonies formed by tens to hundreds of cells into evenly-distributed aggregation with single cell or several cells, thereby meeting requirements of spectrophotometer determination on uniformity of the to-be-detected botryococcus solution. Compared with present methods, the method is rapid, accurate, labour saving and time saving, and provides necessary technical methods for research and development of the botryococcus.

The invention discloses a semi-quantitative detection method for heavy metal ions in a water-soluble sample based on a whole-cell biosensor, and belongs to the technical field of environmental biology. The method is convenient to operate, short in time consumption, low in cost, good in visibility and easy to quantify. The SRRz cracking gene is used as a report element, the response is quick, and the optical density of the bacterial liquid is obviously reduced within 2 hours when the bacterial liquid is in contact with heavy metal. According to the present invention, the SRRz splitting gene is adopted as the report element so as to cause the splitting of the microbial thalli, the correlation between the obtained result and the Cd (II) ion concentration is high, and the detection result can be determined by using naked eyes, and can be detected through a visible light spectrophotometer; x-gal is used for detecting the beta-galactosidase released outside the cells, and the operation is simple and convenient. The method is good in safety, free of reagent addition, free of pigment interference, short in time consumption, low in cost, specific, easy to realize high-throughput screening and convenient to popularize; and technical support can be provided for daily detection and high-throughput screening of heavy metal pollution.

The invention provides a method for producing hexadecanedioic acid through fermentation, and the hexadecanedioic acid and a preparation method thereof. The method for producing hexadecanedioic acid through fermentation comprises the following steps: carrying out amplification culture on candida tropicalis or candida sake until the optical density value OD620 of an obtained seed solution reaches 0.5 or more when the seed solution is diluted by 30 times; inoculating the seed liquid into a fermentation culture medium for fermentation, adding Tween into the fermentation liquid according to a ratioof 0.05-1.00% in the fermentation process, and starting to add n-hexadecane when the optical density value OD620 of the fermentation liquid diluted by 30 times reaches 0.6 or above; and maintaining the pH value of fermentation liquid to be 7 or below in the fermentation process so as to obtain the hexadecanedioic acid. The method for producing hexadecanedioic acid by fermentation provided by theinvention can improve the yield of the hexadecanedioic acid and reduce alkali consumption.

The invention discloses a co-culture method for culturable anaerobic strains and pig intestine epithelial cells. The co-culture method comprises the following steps: measuring an optical density value of a bacterial solution by adopting a spectrophotometric method; determining the concentration of the bacterial liquid by adopting a plate counting method; determining the number of bacteria rapidly through correlation analysis of the two; and inoculating the accurately counted microorganisms to an intestinal epithelial cell line cultured in advance. According to the invention, the interference of various factors in other microorganisms or animal bodies can be eliminated, and the physiological functions of the microorganisms and the interaction mode of the microorganisms and host intestinal epithelial cells can be deeply analyzed.

A serological antibody immunosorbent assay for distinguishing HIV-1 recent and chronic infection, fusion expression MP4 containing partial peptides of HIV-1 B, D, CRF01_AE, CRF07_BC, CRF08_BC transmembrane protein As the detection antigen, the specific antibody representing chronic HIV infection in the sample to be tested is captured, and the optical density value of the blue substance produced by the horseradish peroxidase catalyzed substrate TMB is detected to determine the antibody representing chronic HIV infection in the sample to be tested. Presence and content of anti-HIV antibodies. The fusion-expressed tetravalent polypeptide is used as the detection antigen, which can simultaneously detect multiple HIV genotypes, so that the detection range is wider, and the sensitivity and specificity are higher. The classic ELISA indirect method is used for detection, and the operation is relatively simple. It is more useful in the evaluation of control measures and epidemiological surveys of new infection rates in HIV-1 populations.

The invention discloses a lipoprotein subtyping detection system and method. The system comprises a scanning module, a calibration module, a processing module and an export module; the scanning module is used for scanning a lipoprotein optical density map obtained based on a gel electrophoresis method to obtain a scanning image; the calibration module is used for preprocessing the scanning image to obtain a calibration image; the processing module is used for calculating the cholesterol value of each lipoprotein subtype based on the calibration image; and the export module is used for exporting a processing result obtained by the processing module. According to the invention, accurate detection of cholesterol content of each subtype of lipoprotein can be realized.

The present invention relates to a method for gaining large amounts of data. The data is the output of a device which measures a predetermined parameter. Preferably, the predetermined parameter is the macular pigment optical density (MPOD). The gained data is preferably used to determine the effect of an intervention and / or treatment such as the oral intake of lutein and / or zeaxanthin.