39 results about "OD - Optical density" patented technology

The invention relates to an immunochromatography quantitative detection instrument and a detection method of colloidal gold, latex and the like for quantitative detection of an immunochromatography test strip. A to-be-detected sample is dripped onto a transverse immunochromatography test strip which is marked in advance to coat a corresponding antigen-antibody, then the test strip is inserted into a detection tank of the immunochromatography quantitative detection instrument which is used for confirming the color chromaticity and background chromaticity of C and T line regional positions in a nitrocellulose membrane of the test strip by virtue of multi-wavelength scanning, identification computational analysis is performed on optical density values of a quality control band and a detection band according to a built-in algorithm of the instrument, then the concentration of positive markers contained in the to-be-detected sample is obtained, a report is printed, and a result value is stored. By using the immunochromatography quantitative detection instrument and the detection method, the influence of the difference among different immunochromatography test strips on detection results can be avoided, and precise and stable quantitative detection is really realized. In addition, the immunochromatography quantitative detection instrument and the detection method have the advantages of good open generality and are suitable for determining the detection results of immune test paper with different specifications and different detection types.

The invention discloses a method for monitoring oxygen supply parameters.The optical density (OD) and optical density optical density changing quantity (delta OD) of blood in adjacent descending aortas are detected through an Hb-SO2 sensor arranged in an esophagus via the esophagus wall, and the hemoglobin concentration of blood is obtained through corresponding calculation.The invention further discloses an adopted detecting device.The hemoglobin concentration and even oxygen supply of a patient in the perioperative period can be subjected to routine monitoring like monitoring of SpO2, the oxygen supply of the patient can be kept within a safe range to avoid anoxia injuries and greatly improve the safety of anesthesia and an operation, and meanwhile the effort saving functions of reducing the use frequency of complex invasive monitoring and reducing the work intensity of an anesthetist are achieved.

A method for producing a strain of Saccharomyces cerevisiae with introduced genes coding for xylose reductase, xylitol dehydrogenase and xylulokinase and with improved ethanol production, improved xylose conversion, reduced xylitol production and improved inhibitor tolerance is described. The method comprises culturing a strain of Saccharomyces cerevisiae at a continuous mode with a medium comprising essentially only xylose as carbon source at a temperature of 25-38° C., preferably 30-35° C., and an airflow of 0.040-0.055 vvm, and increasing the dilution rate to maintain a constant cell level, said cell level being in the range of 1.5-3.0 determined by optical density or equivalent analytical means, and adding at least one inhibitor to the cells and gradually increasing the addition of said inhibitor. Further, strains of Saccharomyces cerevisiae obtained by the method according to the invention are described.

The invention relates to a kit for rapid quantitative detection of swine fever antibody. The kit includes a colloidal gold immunochromatography test paper card and a standard curve information card. The colloidal gold immunochromatography test paper card is provided with colloidal gold-labeled hog fever E2-E0 The fusion expression antigen, the colloidal gold-labeled classical swine fever E2-E0 fusion expression antigen is synthesized by connecting the classical swine fever E2-E0 fusion expression antigen and a certain amount of colloidal gold particle solution through physical adsorption, and the colloidal gold particle solution contains The average particle size of colloidal gold is 30-35nm. After the sample to be tested is added to the colloidal gold immunochromatography test paper card, use a color analyzer to read the optical density values ​​of the detection zone and the accusation zone of the test paper card, and then calculate through software according to the standard curve recorded on the standard curve information card. The exact content of swine fever antibody. The invention is easy to operate and produces results quickly, and is a very convenient and good tool for accurately evaluating the immune status of swine fever.

A kind of acetic acid and xylose co-utilization Kluyveromyces marx strain strain, biological deposit number is: CGMCC No.16757. The strain is obtained from Kluyveromyces marxe after long-term directional domestication with cellulose hydrolyzate, and the steps are as follows: Pick a single colony of yeast from a YPD plate and culture overnight at 40°C; re-inoculate the collected cells to a concentration of cellulose hydrolyzate In the fresh medium of liquid, make it have an optical density value at 620nm ≈ 0.3; culture at 40°C, 150rmp / min for 24-48h, when the OD620≥2.0 (5-7 passages), collect the cells; repeat the above steps until Under the stress condition of inhibitors at this concentration, the cell growth rate was significantly improved; the concentration of the cellulose hydrolyzate was increased, and a new repeated culture was started; after continuous acclimatization, the collected cells were spread on the YPD plate containing the cellulose hydrolyzate, Cultivate at 40°C for 24-48h; pick a single colony with good growth status and culture it in YPD liquid medium at 40°C and 150rmp / min for 24-48h; store the dominant resistant clone in 30% glycerol.

The embodiment of the invention relates to the technical field of spectrum detection, and discloses a precise RNA concentration detection method and device, equipment, a medium and an application. The method comprises the steps: receiving total optical densities of collected pollutants at wavelengths of 260 nm, 280 nm and 320 nm; deducting a background reference in the total optical densities to obtain a basic total optical density, and decomposing the basic total optical density; rewriting the formula according to the proportional constants [delta]OD280RNA% and [delta]OD260PRO%, and obtaining the [delta]OD260RNA and the [delta]OD280PRO; and calculating the concentration M of RNA and the concentration N of protein in the pollutants according to the [delta]OD260RNA, the [delta]OD280PRO and the [delta]OD260PRO%. By implementing the embodiment of the invention, the comprehensive spectrum generated by the RNA and the protein in the same pollutant is split, so that the aim of simultaneously detecting the RNA and the protein is fulfilled.

The embodiment of the invention relates to the technical field of spectrum detection, and discloses an accurate DNA concentration detection method, device and equipment, a medium and application. The method comprises the following steps: receiving the total optical densities of collected pollutants at wavelengths of 260 nm, 280 nm and 320 nm; deducting a background reference in the total optical density to obtain a basic total optical density, and decomposing the basic total optical density; re-writting the formula according to the proportional constant delta OD280DNA% and the proportional constant delta OD260PRO% to obtain thedelta OD260DNA and delta OD280PRO; and calculating the concentration M of the DNA and the concentration N of the protein in the pollutants according to the delta OD260DNA, the delta OD280PRO and the delta OD260PRO%. By implementing the embodiment of the invention, the comprehensive spectrum generated by DNA and protein in the same pollutant is split, so that the purpose of simultaneously detecting DNA and protein is achieved.

The invention provides a method for producing hexadecanedioic acid through fermentation, and the hexadecanedioic acid and a preparation method thereof. The method for producing hexadecanedioic acid through fermentation comprises the following steps: carrying out amplification culture on candida tropicalis or candida sake until the optical density value OD620 of an obtained seed solution reaches 0.5 or more when the seed solution is diluted by 30 times; inoculating the seed liquid into a fermentation culture medium for fermentation, adding Tween into the fermentation liquid according to a ratioof 0.05-1.00% in the fermentation process, and starting to add n-hexadecane when the optical density value OD620 of the fermentation liquid diluted by 30 times reaches 0.6 or above; and maintaining the pH value of fermentation liquid to be 7 or below in the fermentation process so as to obtain the hexadecanedioic acid. The method for producing hexadecanedioic acid by fermentation provided by theinvention can improve the yield of the hexadecanedioic acid and reduce alkali consumption.

A serological antibody immunosorbent assay for distinguishing HIV-1 recent and chronic infection, fusion expression MP4 containing partial peptides of HIV-1 B, D, CRF01_AE, CRF07_BC, CRF08_BC transmembrane protein As the detection antigen, the specific antibody representing chronic HIV infection in the sample to be tested is captured, and the optical density value of the blue substance produced by the horseradish peroxidase catalyzed substrate TMB is detected to determine the antibody representing chronic HIV infection in the sample to be tested. Presence and content of anti-HIV antibodies. The fusion-expressed tetravalent polypeptide is used as the detection antigen, which can simultaneously detect multiple HIV genotypes, so that the detection range is wider, and the sensitivity and specificity are higher. The classic ELISA indirect method is used for detection, and the operation is relatively simple. It is more useful in the evaluation of control measures and epidemiological surveys of new infection rates in HIV-1 populations.