SPR-SERS dual-mode sensor for detecting disease nucleic acid markers as well as preparation method and application of SPR-SERS dual-mode sensor

A marker and sensor technology, applied in the fields of biological detection and spectroscopic detection, can solve the problems of missed diagnosis or diagnosis error, nucleic acid amplification, etc., and achieve the effects of reliable detection, good specificity and simple preparation process
CN113293197AActive Publication Date: 2021-08-24NANJING UNIV OF POSTS & TELECOMM
4 Cites 3 Cited by

Patent Information

Authority / Receiving Office
CN · China
Patent Type
Applications(China)
Current Assignee / Owner
NANJING UNIV OF POSTS & TELECOMM
Publication Date
2021-08-24

Smart Images

  • Figure 1
    Figure 1
  • Figure 2
    Figure 2
  • Figure 3
    Figure 3
Patent Text Reader

Abstract

The invention discloses an SPR-SERS dual-mode sensor for detecting a disease nucleic acid marker as well as a preparation method and the application of the SPR-SERS dual-mode sensor. According to the SPR-SERS dual-mode sensor, an SPR sensing mode is realized by extracting integral optical density of dark field color in a dark field microscopic image, and a ratio type SERS sensing mode is realized by acquiring an intensity ratio of SERS characteristic peaks of ROX molecules and 4-mercaptobenzoic acid (4-MBA) molecules. For miRNA-652 detection, nucleic acid single chains C, H1 and H2 in the first reagent, the second reagent and the third reagent are DNA chains which are designed for miRNA-652 and have different nucleotide sequences. The SPR-SERS dual-mode sensor disclosed by the invention is simple to prepare, high in detection sensitivity and good in reliability, the detection limits of an SPR sensing mode and an SERS sensing mode on nucleic acid detection in serum can reach the level of flying mole per liter, and nucleic acid markers related to diseases can be detected in complex biological samples.
Need to check novelty before this filing date? Find Prior Art

Description

technical field

[0001] The invention belongs to the fields of biological detection and spectroscopy detection, and in particular relates to an SPR-SERS dual-mode sensor for detection of gastric cancer nucleic acid marker miRNA-652, a preparation method and application thereof. Background technique

[0002] Accurate detection of trace nucleic acids is essential for reliable analysis of early disease-related biomarkers, which play an important role in disease diagnosis, treatment, and pathogenesis. However, genetic variation due to certain subtle changes in nucleic acid sequence can produce significant biological effects. Therefore, the development of ultrasensitive and reliable methods to detect biomarkers associated with low-abundance diseases is crucial and is becoming an irresistible trend. Traditional analytical methods such as Northern blot hybridization, microarray technology, and real-time quantitative polymerase chain reaction (PCR) have been widely used in nucleic a...

Claims

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
Login to View More