Diagnostic kit and application of MAK16 in preparation of early diagnostic reagent against systemic lupus erythematosus

A diagnostic kit, lupus erythematosus technology, applied in the field of biological sciences, can solve problems such as the inability to achieve early diagnosis of SLE with high sensitivity and high specificity

Active Publication Date: 2020-01-14
ANHUI MEDICAL UNIV
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  • Abstract
  • Description
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  • Application Information

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Problems solved by technology

[0006] The first purpose of the present invention is to provide a diagnostic kit to solve the problem that the diagnostic kits for systemic lupus erythematosus in the prior art cannot realize the early detection of SLE Technical Issues of Sensitive, Highly Specific Diagnosis
[0007] The second purpose of the present invention is to provide a kind of application of MAK16 in the preparation of systemic lupus erythematosus early diagnosis reagent, to solve the systemic lupus erythematosus in the prior art The technical problem that the diagnostic kit cannot realize the early diagnosis of SLE with high sensitivity and high specificity

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  • Diagnostic kit and application of MAK16 in preparation of early diagnostic reagent against systemic lupus erythematosus

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Embodiment Construction

[0024] A diagnostic kit proposed by the present invention and the application of MAK16 in the preparation of early diagnostic reagents for systemic lupus erythematosus will be further described in detail below with reference to the accompanying drawings and specific examples. Advantages and features of the present invention will be apparent from the following description and claims.

[0025]MAK16 is called RNA binding protein 13 (RNA binding protein 13, RBM13), which is located in the nucleus and participates in key processes such as RNA cleavage, transport, localization, editing, and post-transcriptional regulation of mRNA (Andersen JS, et al: Current Biology Cb, 2002, 12(1):1-11). Some researchers observed through cryo-electron microscopy that MAK16 was involved in the formation and stabilization of the ribosomal 60S large subunit precursor (Kater L, et al: Cell, 2017, 171(7):1599-1610e14); According to related studies on Saccharomyces cerevisiae, it is considered to be inv...

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Abstract

The invention discloses a diagnostic kit and an application of MAK16 in preparation of an early diagnostic reagent against systemic lupus erythematosus (SLE). The diagnostic kit can be used for earlydiagnosis of systemic lupus erythematosus disease. The kit includes an enzyme-labeling plate, human protein MAK16 as an antigen, a coating buffer, standard serum, an enzyme-labeling reagent, a confining liquid, and a sample diluent. By coating the human protein MAK16 on the enzyme-labeling plate, optical density of a IgG antibody of the anti-human protein MAK16 in serum is tested to assist diagnosis of systemic lupus erythematosus. The invention provides the sensitive, safe, reliable, and easy-to-operate commercial kit, helpful for auxiliary diagnosis and differential diagnosis of SLE by qualitatively detecting the level of the IgG antibody of the anti-human protein MAK16 in the human serum. The kit provided by the invention is used for screening or initial diagnosis of SLE disease with 88.12% of specificity and 48.98% of sensitivity, and used for differential diagnosis of SLE disease with 86.50% of specificity and 39.50% of sensitivity. The kit can be used as a helpful supplement to existing SLE diagnostic markers.

Description

technical field [0001] The invention belongs to the field of biological sciences, and in particular relates to a diagnostic kit and the application of MAK16 in the preparation of early diagnostic reagents for systemic lupus erythematosus. Background technique [0002] Systemic lupus erythematosus (SLE) is a chronic autoimmune disease that may damage multiple systems and organs of the whole body and seriously threatens human health. There are large differences, among which the incidence rate of Asians and Americans is higher (Rees F, et al: Rheumatology (Oxford), 2017, 56(11):1945-1961), and its global average prevalence rate is about 17 / 100,000 ~48 / 100,000, the prevalence rate in China is about 40 / 100,000~70 / 100,000, and the ratio of male to female is about 1:9. Irreversible damage to various organs or systems, among which the more common damage is the skin and mucous membranes, kidneys, blood system, nervous system, and joints (Wu H, et al: Autoimmunity reviews, 2017, 16(4...

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): G01N33/537G01N33/68
CPCG01N33/537G01N33/6893G01N2800/104
Inventor 叶冬青凌华志
Owner ANHUI MEDICAL UNIV
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