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Sample pretreatment and determination method for detecting optical density values of botryococcus

A technology of optical density value and grape algae, which is applied in the preparation of test samples, color/spectral characteristic measurement, etc., can solve the problems of uniform distribution, slow research and development progress, affecting the feasibility and accuracy of optical density value, etc.

Inactive Publication Date: 2013-07-10
ZHEJIANG UNIV
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Problems solved by technology

This colony-like cell mass is difficult to distribute evenly in the culture medium due to its large size and easy sinking, which seriously affects the feasibility and accuracy of optical density measurement.
Because of this, the traditional dry weight method is still used in the research and development of botrytis to detect its biomass, which is also one of the important reasons for the slow progress in research and development of botrytis breeding, cultivation and deep processing at home and abroad for many years [Yaming Ge, et al. Growth characteristics of Botryococcus braunii 765 under high CO 2 concentration in photobioreactor. Bioresource Technology. 2011, 102(1): 130–134]

Method used

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Embodiment Construction

[0018] When using a spectrophotometer to measure the optical density of microalgae, it is required that the algal cells can be evenly distributed in the culture medium. In order to prepare the botrytis in the form of colonies into uniformly distributed single cells or aggregates of several cells, the method uses ultrasonic waves with appropriate power to make them dispersed as much as possible. At the same time, in order to reduce the mechanical damage of ultrasonic waves to algae cells, and to overcome the problem that the volume and density of botrytis cells are larger than those of microalgae cells such as chlorella and are easy to sink, this method adds a small amount of Xanthan gum (Xanthan gum), achieved very good results. This is mainly based on the following three characteristics of Xanthomonas polysaccharide: (1) It has good water solubility, is transparent and colorless, and has stable performance. A membrane is formed on the surface of algae cells, which can make t...

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Abstract

The invention relates to a technology of development and utilization of botryococcus, and aims at providing a sample pretreatment and determination method for detecting optical density values of the botryococcus. The method comprises putting to-be-detected botryococcus into a centrifuge tube, adding particle-type Xanthomonas polysaccharide, putting in an ice-water bath, and treating with ultrasonic to make botryococcus colonies formed by tens to hundreds of cells into evenly-distributed aggregation with single cell or several cells, thereby meeting requirements of spectrophotometer determination on uniformity of the to-be-detected botryococcus solution. Compared with present methods, the method is rapid, accurate, labour saving and time saving, and provides necessary technical methods for research and development of the botryococcus.

Description

technical field [0001] The invention belongs to the technique of developing and utilizing botrytis, in particular to a sample pretreatment and determination method for detecting the optical density value of botrytis. Background technique [0002] Microalgae is one of the earliest photosynthetic autotrophs on the earth. It not only plays a vital role in the global material and energy conversion, ecosystem balance, etc. Basic microalgae industry, and used in food, medicine, feed, chemical industry, environmental protection, energy, materials and other industries. The optical density method based on the principle and technology of spectrophotometry is a common and effective method for rapid, simple and high-throughput determination of microbial biomass such as microalgae at home and abroad. It plays an irreplaceable role in microalgae breeding, cultivation, and deep processing. The important role of [Wang Yingjuan et al. Journal of Northwest University (Natural Science Edition...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): G01N1/28G01N21/31
Inventor 汪志平刘新颖于金鑫吕蓓芬马丽芳陈子元
Owner ZHEJIANG UNIV
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