Image-analysis-based cancer cell DNA content detection method

A tumor cell and detection method technology, which is applied in the field of tumor cell DNA content detection based on image analysis, can solve the problems of cumbersome detection, inability to timely diagnose tumor cells of patients, and long detection time, and achieve fast measurement, convenient and quick diagnosis, and low cost. low effect

Inactive Publication Date: 2017-06-20
SICHUAN UNIV
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Problems solved by technology

[0008] Traditional methods for detecting the DNA content of tumor cells include sugar determination, spectrophotometry, etc. Due to the cumb

Method used

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  • Image-analysis-based cancer cell DNA content detection method
  • Image-analysis-based cancer cell DNA content detection method
  • Image-analysis-based cancer cell DNA content detection method

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Example Embodiment

[0037] See figure 1 .

[0038] The hardware device of the present invention is mainly composed of a microscope, a camera, a desktop computer and a printer. The microscope uses a transmission biological microscope, the camera uses a high-definition digital camera, the computer uses a desktop computer, and the printer uses a color laser printer. The camera is installed on the upper part of the microscope and is connected to the microscope through the microscope camera interface to collect images of the field of view observed under the microscope; the camera is connected to the USB port of the computer through a data cable, and the pathological slice image observed by the camera is transmitted to the computer; The printer is connected to the computer through a USB port, and the DNA test report generated by the analysis software is printed out on the printer.

[0039] See attached Figure 2-4 .

[0040] Tumor cell DNA content detection methods include five steps: length calibration, b...

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Abstract

The invention discloses a method for detecting DNA content of tumor cells based on image analysis. In this method, a camera installed on the upper part of the microscope collects sliced ​​images observed under the microscope and transmits them to a computer, and the images are analyzed and measured by analysis software. The process is: collect the scale image and calibrate the length; collect the background image and measure the pixel gray value of the background image; collect the normal diploid cell image, perform image segmentation on the diploid cell image, and measure the gray value of the diploid cell nucleus. Calculate the average optical density value of the diploid cell nucleus; collect tumor cell images, segment the tumor cell image, measure the optical density value of each tumor cell nucleus pixel, and calculate the DI value and area of ​​each tumor cell nucleus; generate DNA histogram and scatter diagram, count the number of various cells according to DI classification, and generate a DNA test report. The method has the advantages of low cost, fast measurement, reduction of artificial judgment errors, easy preservation of pictures and the like.

Description

technical field [0001] The invention relates to the field of biomedicine and the application field of computer image analysis, in particular to a method for detecting DNA content of tumor cells based on image analysis. Background technique [0002] In recent years, with the increasingly serious problems of environmental pollution and food safety, the incidence of cancer in my country is on the rise. According to reports, in 2015, about 4.3 million people in my country were diagnosed with cancer, and another 2.8 million people died of cancer. Cancer has also become a major public health problem in our country because of its extremely high mortality rate. How to detect cancer earlier and more accurately is of great significance to reduce the mortality of cancer patients. [0003] There are many methods for the diagnosis of cancer tumors, among which the quantitative analysis of DNA is of great significance in helping to discover precancerous lesions and assist in the early di...

Claims

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Application Information

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IPC IPC(8): G06T7/00G06T5/40G06T7/10
CPCG06T7/0012G06T5/40G06T2207/10061G06T2207/30096
Inventor 邱敦国谢丹
Owner SICHUAN UNIV
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