The invention discloses an automatic cross-matching testing method suitable for known blood types. Before the blood transfusion, a professional examiner needs to finish a cross-matching test in a specialized laboratory. A tester comprises an upper-part shell, a lower-part shell, a needle ejection device, a hemostix, a needle ejector, a reagent absorbing and adding mechanism, a base, a centrifugaltest mechanism, a blood matching test tube, a microscope camera, an extraction pipe hand claw, a test tube hand claw for the test, a test tube hand claw for a blood receptor, a test tube storage box,an extraction tube storage box, a waste test tube collection box and a waste extraction tube collection box, wherein the centrifugal test mechanism comprises an upper disc, a support disc, a centrifugal rotating shaft, a positioning rotating shaft and a lifting shaking assembly; and the reagent absorbing and adding mechanism comprises a reagent disc, a layering collector, a condensed amine mediumreagent storage, a serum guiding pipe, a normal saline storage box, a first serum storage box and a second serum storage box. According to the method, the blood type detection and cross-matching testcan be automatically finished, and the workload of medical workers in the blood matching process is simplified.