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Method for producing hexadecanedioic acid through fermentation, and hexadecanedioic acid and preparation method of hexadecanedioic acid

A dibasic acid and sixteen carbon technology, applied in the field of fermentation, can solve the problems of high sewage treatment cost, low production efficiency, large alkali consumption, etc., and achieve the effect of reducing sewage treatment cost, shortening production cycle and reducing production cost

Pending Publication Date: 2020-10-30
CATHAY R&D CENT CO LTD +1
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

In order to maintain the fermentation system at a weak alkaline level, it is often necessary to continuously add alkaline substances such as sodium hydroxide, which not only consumes a lot of alkali, but also easily causes downstream pollution, and the cost of sewage treatment is also high
In view of this present situation, in the patent US 6569670 B2, the technique of producing hexadecanoic acid under weakly acidic conditions has been reported, but under the condition of low pH value, the activity of P450 enzyme is low, the metabolism is slow, and the production efficiency is low. The high price of n-hexadecane raw materials leads to the high cost of hexadecane dibasic acid, so the process is difficult to be suitable for industrial production

Method used

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  • Method for producing hexadecanedioic acid through fermentation, and hexadecanedioic acid and preparation method of hexadecanedioic acid
  • Method for producing hexadecanedioic acid through fermentation, and hexadecanedioic acid and preparation method of hexadecanedioic acid

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1～12

[0056] Embodiments 1 to 12 each provide a method for producing hexadecanoic acid by fermentation, which is to use Candida sake (Candidasake) CAT H4014 in a 10L fermenter, and add 0.05% to 0.20% when the fermentation starts 30 to 50 hours Tween-60 specifically comprises the following steps:

[0057] 1. First adjust the pH value of the 10 Baume wort to about 5.4, then take 80mL into a 500mL Erlenmeyer flask, sterilize at 121°C for 20min, and then insert it into the previously stored in a -80°C refrigerator One seed of Candidasake (Candidasake) in a 2mL glycerol tube was activated and cultured on a rotary shaker at a temperature of 29°C and a rotation speed of 250rpm for 40-44h, and the cell OD 620 When the value reaches above 0.5 (diluted 30 times), the activation culture ends.

[0058] 2. Insert the shake flask seeds obtained in the above step 1 into the seed tank containing the seed medium and cultivate them. The inoculum size is 1.33%, and the temperature during the cultivat...

Embodiment 13～24

[0083] Examples 13-24 each provide a method for fermenting and producing hexadecanoic acid, using Candida Tropicalis CAT H1614 in a 10L fermenter, and adding 0.05-0.20% Tween-80 specifically comprises the following steps:

[0084] 1. First adjust the pH value of the 10 Baume wort to about 5.4, then take 80mL into a 500mL Erlenmeyer flask, sterilize at 121°C for 20min, and then insert it into the previously stored in a -80°C refrigerator One seed of Candida Tropicalis in a 2mL glycerol tube was activated and cultured on a rotary shaker at a temperature of 29°C and a rotation speed of 250rpm for 40-44h, and the OD of the bacteria was 620 When the value reaches above 0.5 (diluted 30 times), the activation culture ends.

[0085] 2. Insert the shake flask seeds obtained in the above step 1 into the seed tank containing the seed medium and cultivate them. The inoculum size is 1.33%, and the temperature during the cultivation process is controlled to be 29°C, the air volume is 0.3vv...

Embodiment 25

[0111] Example 25 provides a method for fermenting and producing hexadecanoic acid, using Candidasake CAT H4014 in a 200 cubic meter fermenter, except that 0.10% Tween-60 is added at 40 hours of fermentation , other process conditions are consistent with embodiment 1.

[0112] When 0.10% Tween-60 is added to the fermentation broth, during the process of adding n-hexadecane to carry out fermentation and conversion, the emulsification layer in the fermentation broth is observed by sampling, and it is found that the emulsification layer in the fermentation broth after adding Tween-60 more.

[0113] After the fermentation was stopped, the fermentation period was 136 hours, and the fermentation liquid was detected, and the content of hexadecanoic acid in the fermentation liquid was 178.3 g / L, and the conversion rate was 88.5%.

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Abstract

The invention provides a method for producing hexadecanedioic acid through fermentation, and the hexadecanedioic acid and a preparation method thereof. The method for producing hexadecanedioic acid through fermentation comprises the following steps: carrying out amplification culture on candida tropicalis or candida sake until the optical density value OD620 of an obtained seed solution reaches 0.5 or more when the seed solution is diluted by 30 times; inoculating the seed liquid into a fermentation culture medium for fermentation, adding Tween into the fermentation liquid according to a ratioof 0.05-1.00% in the fermentation process, and starting to add n-hexadecane when the optical density value OD620 of the fermentation liquid diluted by 30 times reaches 0.6 or above; and maintaining the pH value of fermentation liquid to be 7 or below in the fermentation process so as to obtain the hexadecanedioic acid. The method for producing hexadecanedioic acid by fermentation provided by theinvention can improve the yield of the hexadecanedioic acid and reduce alkali consumption.

Description

technical field [0001] The invention belongs to the technical field of fermentation, and in particular relates to a method for producing hexadecanoic acid by fermentation, hexadecanoic acid and a preparation method thereof, in particular to a method for producing hexadecanoic acid by biological fermentation under acidic conditions process method. Background technique [0002] Long-chain dibasic acids (also known as long-chain dibasic acids) are aliphatic dicarboxylic acids with more than 10 carbon atoms in the carbon chain, and the general molecular formula is C n h 2n-2 o 4 , n≥10. Long-chain dibasic acid is a kind of fine chemical product with a wide range of uses. Using long-chain dibasic acid or its derivative monomer as raw material, it can be used to synthesize super-grade nylon, cold-resistant plasticizer, high-grade hot-melt adhesive, powder coating, spices, etc. . Especially hexadecanoic acid (α, ω-dicarboxylic long-chain dibasic acid, referred to as DC 16 ), ...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): C12P7/64C12N1/16C12R1/74C12R1/72
CPCC12P7/6409C12N1/16
Inventor 鲍家伟赵宏图徐敏刘修才
Owner CATHAY R&D CENT CO LTD
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