Method for analyzing plankton community DNA fingerprint in urban sewage

A technology of DNA fingerprinting and plankton, which is applied in the direction of biochemical equipment and methods, measurement/inspection of microorganisms, etc., can solve problems such as neglect, and achieve the effect of good repeatability, heavy workload and high sensitivity

Inactive Publication Date: 2008-09-10
INST OF AQUATIC LIFE ACAD SINICA
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  • Abstract
  • Description
  • Claims
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Problems solved by technology

However, fungi and other eukaryotes, which also play an important role in sewage treatment, are often overlooked.

Method used

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  • Method for analyzing plankton community DNA fingerprint in urban sewage
  • Method for analyzing plankton community DNA fingerprint in urban sewage
  • Method for analyzing plankton community DNA fingerprint in urban sewage

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0026] In a sewage treatment plant in Wuhan, five sampling points of sewage inlet (I), anaerobic tank (II), anoxic tank (III), aeration tank (IV), and secondary sedimentation tank outlet (V) were collected. samples were collected and analyzed (see appendix figure 2 ).

[0027] 1. Metagenomic DNA Extraction of Plankton Community A

[0028] Directly use the water collector to collect urban sewage water samples, mix the water samples thoroughly, transfer the mixed water samples to a 1.5mL centrifuge tube, centrifuge to obtain the precipitate, and remove the supernatant, the speed is 10000r / min, the time is 5min, and then Wash the precipitate with sterilized double-distilled water, centrifuge once more, and suspend the obtained precipitate in 1.5 mL of sterilized TENP buffer, the TENP buffer contains: 50mmol / L Tris-base; 20mmol / L EDTA; 100mmol / LNaCl; 0.01 g / mL polyvinylpyrrolidone; pH=10, then the precipitated suspension was shaken and mixed, and the supernatant was removed by ...

Embodiment 2

[0038] During the operation of a sewage treatment plant in Wuhan, activated sludge foaming and expansion occurred, and a large number of difficult-to-remove foams appeared in the biological pool. Since there are many reasons for the expansion of activated sludge, the present invention is used to detect the plankton community structure in the water samples in the anaerobic section, the anoxic section and the aeration section in the biological pool to determine the cause of the expansion. The water samples before and after the expansion of activated sludge were used for detection, with a time interval of 15 days.

[0039] 1. Metagenomic DNA Extraction of Plankton Community A

[0040] The activated sludge suspension samples were directly collected from the three parts of the anaerobic section (II), the anoxic section (III), and the aeration section (IV) in the biological pool by using the water collector. The water sample was fully mixed, and the mixed water sample was transferr...

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Abstract

The invention relates to an analysis method for plankton type community DNA dactylogram in urban sewage, the method is as follows: metagenome of plankton type community in urban sewage is amplified through using a primer aiming at 16S rDNA and 18S rDNA, and undergoes denaturing gradient gel electrophoresis to obtain a fingerprinting of community DNA. Firstly, the metagenome DNA of the plankton type community is extracted; secondly, the object gershgorim band of the metagenome is amplified; thirdly, the DGGE electrophoretic separation is performed to obtain the fingerprinting of the plankton type community DNA; fourthly, a DGGE gel picture is analyzed through using DNA UVI software, using the optical density value of the gershgorim band to indicate the gershgorim band; and finally, data analysis is performed through using statistics software. The analysis method for plankton type community DNA dactylogram in urban sewage has the advantages of convenience and efficiency, excellent repeatability, high sensitivity and low cost, capability of being applied to biological monitoring of urban sewage.

Description

technical field [0001] The present invention relates to an analysis method in the field of microbial molecular ecology, in particular to a technique for analyzing DNA fingerprints of plankton biocommunities in urban sewage by using PCR-DGGE, that is, analyzing the DNA of plankton community in urban sewage by PCR-DGGE. State, using its polymorphism to analyze the relationship between various sewage treatment stages and the operation status of activated sludge. Background technique [0002] The secondary sewage treatment process of urban sewage treatment plants is mostly activated sludge method and its improved process. The principle is to treat organic pollutants through a complex ecosystem composed of various bacteria, fungi, algae and protozoa. Biodegradation, while removing excess phosphorus and nitrogen in sewage, adsorbing heavy metal ions and even planktonic viruses, etc., to achieve the purpose of purifying water quality. Sewage treatment plants usually only detect ro...

Claims

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Application Information

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IPC IPC(8): C12Q1/68
Inventor 余育和张翔冯伟松颜庆云
Owner INST OF AQUATIC LIFE ACAD SINICA
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