Method for analyzing plankton community DNA fingerprint in urban sewage
A technology of DNA fingerprinting and plankton, which is applied in the direction of biochemical equipment and methods, measurement/inspection of microorganisms, etc., can solve problems such as neglect, and achieve the effect of good repeatability, heavy workload and high sensitivity
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Embodiment 1
[0026] In a sewage treatment plant in Wuhan, five sampling points of sewage inlet (I), anaerobic tank (II), anoxic tank (III), aeration tank (IV), and secondary sedimentation tank outlet (V) were collected. samples were collected and analyzed (see appendix figure 2 ).
[0027] 1. Metagenomic DNA Extraction of Plankton Community A
[0028] Directly use the water collector to collect urban sewage water samples, mix the water samples thoroughly, transfer the mixed water samples to a 1.5mL centrifuge tube, centrifuge to obtain the precipitate, and remove the supernatant, the speed is 10000r / min, the time is 5min, and then Wash the precipitate with sterilized double-distilled water, centrifuge once more, and suspend the obtained precipitate in 1.5 mL of sterilized TENP buffer, the TENP buffer contains: 50mmol / L Tris-base; 20mmol / L EDTA; 100mmol / LNaCl; 0.01 g / mL polyvinylpyrrolidone; pH=10, then the precipitated suspension was shaken and mixed, and the supernatant was removed by ...
Embodiment 2
[0038] During the operation of a sewage treatment plant in Wuhan, activated sludge foaming and expansion occurred, and a large number of difficult-to-remove foams appeared in the biological pool. Since there are many reasons for the expansion of activated sludge, the present invention is used to detect the plankton community structure in the water samples in the anaerobic section, the anoxic section and the aeration section in the biological pool to determine the cause of the expansion. The water samples before and after the expansion of activated sludge were used for detection, with a time interval of 15 days.
[0039] 1. Metagenomic DNA Extraction of Plankton Community A
[0040] The activated sludge suspension samples were directly collected from the three parts of the anaerobic section (II), the anoxic section (III), and the aeration section (IV) in the biological pool by using the water collector. The water sample was fully mixed, and the mixed water sample was transferr...
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