Method for detecting production trait goodness and badness of spirulina strain

A technology for producing traits and spirulina, applied in measuring devices, material analysis by electromagnetic means, instruments, etc., can solve the problems of high cost, long cycle, cumbersome procedures, etc., and achieve the effect of low cost

Inactive Publication Date: 2013-04-03
ZHEJIANG UNIV
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Although this method is practical and effective, the procedure is c...

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  • Method for detecting production trait goodness and badness of spirulina strain
  • Method for detecting production trait goodness and badness of spirulina strain
  • Method for detecting production trait goodness and badness of spirulina strain

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[0022] Sodium dodecylsulfonate-polyacrylamide gel electrophoresis (SDS-PAGE) technology of protein has been widely used in the classification and identification of tobacco, rice, woody plants and seaweed, etc. However, there are few reports on the application of SDS-PAGE fingerprints and related analysis methods in classification and identification. The invention establishes a new method for detecting the optical density value of the band at 102kD in the protein fingerprint, and identifying the excellent production properties of spirulina and whether it can be applied to large-scale production and breeding through clustering.

[0023] We used 9 well-known strains of Spirulina ZJU0101, ZJU0102, ZJU0104, ZJU0105, ZJU0106, ZJU0107, Sp-Js, ZJU0114 and ZJU0115 as control materials, and detected their protein electrophoretic patterns at 102kD by SDS-PAGE and Quantity One software. According to the size of the optical density value of the protein band, cluster analysis was carried ou...

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Abstract

The invention relates to the technique of development and application of spirulina and aims at providing a method for detecting production trait goodness and badness of spirulina strains. According to the method, a Tris-HC1 extracting solution is utilized for extraction so as to obtain water-soluble protein of spirulina cells; protein samples are separated by using sodium dodecyl sulfate-polyacrylamide gel electrophoresis, subsequently the optical density value of a protein zone at 102kD of a protein electrophoresis diagram is detected, and a dendrogram of the spirulina plants is established according to the value; if the optical density value of a candidate strain at the protein zone at the 102kD is larger than 140 and the candidate strain is gathered together with a known stain with good production nature, the stain is good in production nature and is applicable to large-scale cultivation and production; and if the optical intensity value of the protein zone at 102kD is less than 40 and the candidate strain is gathered together with known strains with poor production nature, the stain is poor in production nature and is not applicable to large-scale cultivation and production. The method is simple, efficient, reliable and low in cost, and complex tests and analysis on nucleic acid sequencing and bioinformatics comparison do not need to be carried out, therefore, the method is applicable to large-scale and high flux separation.

Description

technical field [0001] The invention belongs to the technology for the development and application of spirulina, in particular to a method for identifying the quality of production traits of spirulina strains. Background technique [0002] Spirulina is a photosynthetic, oxygen-releasing, regular spiral prokaryotic filamentous microalgae, a genus of Cyanophyta, Oscillatoriales, and Oscillatoriaceae [Wuhan Botany Research, 1997, 15(4): 369-374]. Because it is rich in high-quality protein and a variety of biologically active substances, it has attracted great attention at home and abroad, and has formed a huge spirulina industry on the basis of a large number of researches, becoming the world's largest research and development scale and the most extensive application prospects. microalgae. It is worth pointing out that numerous experimental studies and long-term production practices have shown that different varieties (strains) of Spirulina have different requirements and ada...

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Application Information

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IPC IPC(8): G01N27/447
Inventor 汪志平于金鑫刘新颖吕蓓芬马丽芳陈子元
Owner ZHEJIANG UNIV
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