A method for constructing a pH-resolving colorimetric biosensor

A biosensor and colorimetric technology, applied in the field of pH-resolution colorimetric biosensors, can solve problems such as global food pollution, adverse effects on humans, animals and crops, diseases and economic losses

Active Publication Date: 2020-08-28
JIANGSU UNIV
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0004] Global contamination of food by mycotoxins is a major concern as they adversely affect humans, animals and crops, leading to disease and economic loss

Method used

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  • A method for constructing a pH-resolving colorimetric biosensor
  • A method for constructing a pH-resolving colorimetric biosensor
  • A method for constructing a pH-resolving colorimetric biosensor

Examples

Experimental program
Comparison scheme
Effect test

example 1

[0108] 1) Ferric oxide nanoparticles and graphene oxide composite (Fe 3 o 4 / GO) preparation

[0109] First, 50mL graphene oxide aqueous solution (0.8mg / mL) was sonicated for 1h, followed by nitrogen gas for 30min; then, under nitrogen protection, 50mL ferric chloride (0.055g) and ferrous sulfate (0.048g) mixed solution was added dropwise, and stirred for 12h . Stir vigorously, add 6M sodium hydroxide dropwise to the mixture until the pH value reaches 10.0, react at 90°C for 1.5h, and then cool to room temperature. Wash with phosphate buffer with a pH value of 7.4 until neutral, and store at 100 mL at 4°C for later use.

[0110] 2) Colorimetric analysis at different temperatures

[0111] The fixed reaction time is 90min, pipette 1mL PP-DNA respectively 1 -GO-OTA aptamer-DNA 2 -Fe 3 o 4 / GO, MGCB-DNA 3 -GO-AFB1aptamer-DNA 4 -Fe 3 o 4 / GO solution mixed, remove the supernatant after magnetic separation, add 1mL target ochratoxin A (OTA) and aflatoxin B1 (AFB1) mixtur...

example 2

[0113] 1) Ferric oxide nanoparticles and graphene oxide composite (Fe 3 o 4 / GO) preparation

[0114] First, 50mL graphene oxide aqueous solution (0.8mg / mL) was sonicated for 1h, followed by nitrogen gas for 30min; then, under nitrogen protection, 50mL ferric chloride (0.055g) and ferrous sulfate (0.048g) mixed solution was added dropwise, and stirred for 12h . Stir vigorously, add 6M sodium hydroxide dropwise to the mixture until the pH value reaches 10.0, react at 90°C for 1.5h, and then cool to room temperature. Wash with phosphate buffer with a pH value of 7.4 until neutral, and store at 100 mL at 4°C for later use.

[0115] 2) Colorimetric analysis at different times

[0116] The fixed reaction temperature was 37°C. , respectively pipette 1mL PP-DNA 1 -GO-OTA aptamer-DNA 2 -Fe 3 o 4 / GO, MGCB-DNA 3 -GO-AFB1aptamer-DNA 4 -Fe 3 o 4 / GO solution mixed, remove the supernatant after magnetic separation, add 1mL target ochratoxin A (OTA) and aflatoxin B1 (AFB1) mix...

example 3

[0118] 1) Ferric oxide nanoparticles and graphene oxide composite (Fe 3 o 4 / GO) preparation

[0119] First, 50mL graphene oxide aqueous solution (0.8mg / mL) was sonicated for 1h, followed by nitrogen gas for 30min; then, under nitrogen protection, 50mL ferric chloride (0.055g) and ferrous sulfate (0.048g) mixed solution was added dropwise, and stirred for 12h . Stir vigorously, add 6M sodium hydroxide dropwise to the mixture until the pH value reaches 10.0, react at 90°C for 1.5h, and then cool to room temperature. Wash with phosphate buffer with a pH value of 7.4 until neutral, and store at 100 mL at 4°C for later use.

[0120] 2) Colorimetric detection of ochratoxin A (OTA) and aflatoxin B1 (AFB1)

[0121] The fixed reaction temperature was 37 °C and the reaction time was 90 min. , respectively pipette 1mL PP-DNA 1 -GO-OTAaptamer-DNA 2 -Fe 3 o 4 / GO, MGCB-DNA 3 -GO-AFB1aptamer-DNA 4 -Fe 3 o 4 / GO solution was mixed, and after magnetic separation, the supernatant ...

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Abstract

The invention belongs to the field of construction of a colorimetric biosensor, and provides a method for constructing a pH distinguishing colorimetric biosensor. A technical scheme comprises the following steps: 1) preparing ferriferrous oxide nano particles and a graphene oxide compound (Fe3O4 / GO); and 2) constructing the pH distinguishing colorimetric biosensor. The pH distinguishing colorimetric biosensor provides a simple, rapid, accurate and low-cost strategy for multi-target detection.

Description

technical field [0001] The invention belongs to the field of colorimetric biosensor construction, and in particular relates to a method for constructing a simple, accurate, low-cost pH-resolution colorimetric biosensor for simultaneous detection of multiple targets. Background technique [0002] Due to the advantages of low cost, easy portability, and simple operation, colorimetric sensing has been widely used in daily life to detect various targets, such as: DNA, protein, cells, toxins, heavy metal ions, etc. The main reason is that colorimetric sensing does not require complex instruments, and the quantification of targets can be achieved by naked eyes, so it is very suitable for test points or field tests. [0003] For colorimetric sensors, current designs are primarily based on a single color change, severely limiting the ability to detect multiple objects. So far, there are few reports on colorimetric biosensors capable of multi-target detection. Chromogenic dyes can ...

Claims

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Application Information

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Patent Type & Authority Patents(China)
IPC IPC(8): G01N21/80
CPCG01N21/80
Inventor 郝楠王坤周舟
Owner JIANGSU UNIV
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