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A self-assembled nanosystem loaded with sea cucumber saponin and its preparation method and application

A sea cucumber saponin and self-assembly technology, applied in the biological field, can solve the problems of reducing the hemolytic toxicity of sea cucumber saponin

Active Publication Date: 2022-06-03
QINGDAO UNIV OF SCI & TECH
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

However, there is no report on the use of 1,6-α-D-glucan to reduce the hemolytic toxicity of sea cucumber saponins

Method used

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  • A self-assembled nanosystem loaded with sea cucumber saponin and its preparation method and application
  • A self-assembled nanosystem loaded with sea cucumber saponin and its preparation method and application
  • A self-assembled nanosystem loaded with sea cucumber saponin and its preparation method and application

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0044] With anhydrous KBr tablet, carry out infrared spectrum analysis. The infrared spectra of dextran and palmitoylated dextran are shown in Figure 1.

[0049] The preparation of the hemolytic toxic red blood cell suspension: get the mouse red blood cells, and prepare a 2% red blood cell suspension for subsequent use.

Embodiment 2

[0055] Tested sample: the self-assembly system of sea cucumber saponin palmitoylated glucan prepared in Example 1.

[0058] Reagents and instruments: trypsin, MTT, microplate reader.

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Abstract

The invention discloses a self-assembled nano system containing sea cucumber saponin, a preparation method and application thereof, and belongs to the field of biotechnology. The present invention first utilizes 1,6-α-D-glucan to react with palmitoyl chloride to generate palmitoylated glucan, separates and purifies to obtain palmitoylated glucan compound; then dissolves the obtained palmitoylated glucan in A thin film is made in ethanol, a phosphate buffer solution containing sea cucumber saponin is added, and stirred to form a palmitoylated dextran self-assembly system containing sea cucumber saponin. The encapsulation rate of the palmitoylated dextran self-assembly system loaded with sea cucumber saponin prepared by the invention is 69%, and the hemolysis rate is less than 5% when the sea cucumber saponin concentration is 600 μg / mL, which meets the requirements of injection medicine. The cytotoxicity test proves that the sea cucumber saponin-loaded palmitoylated glucan self-assembly system prepared by the invention has the effect of inhibiting tumor cells, and can be used to prepare antitumor drugs.

Description

A kind of self-assembled nano system containing sea cucumber saponins and preparation method and application thereof technical field The present invention relates to the field of biotechnology, particularly relate to a kind of self-assembled nano system of sea cucumber saponins and its Preparation method and application. Background technique [0002] Sea cucumber saponins are important secondary metabolites in the body of sea cucumber, and have various pharmacological activities such as antitumor and antifungal. In recent years, the good antitumor activity of sea cucumber saponins has gradually attracted everyone's attention. But oral sea cucumber saponins are easily digested by the digestive system Enzymatic degradation within it affects its bioavailability. Intravenous administration of sea cucumber saponins because of their ability to bind to sterol molecules on biofilms Combined, the perforation of red blood cells causes hemolysis, and the above reasons limit the...

Claims

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Application Information

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Patent Type & Authority Patents(China)
IPC IPC(8): A61K9/51A61K47/36A61K31/7048A61P35/00
CPCA61K9/5161A61K31/7048A61P35/00
Inventor 李红燕解万翠杨锡洪车红霞董秀芳宋琳
Owner QINGDAO UNIV OF SCI & TECH
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