Magnetic bead drying protection liquid, dried magnetic bead and preparation method of dried magnetic bead

Abstract

The invention relates to the technical field of biochemical reagents, and in particular, relates to a magnetic bead drying protection liquid, a dried magnetic bead and a preparation method of the dried magnetic bead. The magnetic bead drying protection liquid comprises the components in percentage by mass: 5 to 15 percent of cellobiose, 0.005 to 0.02 percent of Tween 20, 0.05 to 0.2 percent of sucrose ester and the balance being water; the HLB value of the sucrose ester is 15. The magnetic bead drying protection liquid provided by the invention can protect the performance of the dried hydroxyl magnetic bead to the greatest extent, the dried hydroxyl magnetic bead can be stored and transported at room temperature, the storage life is long, and the magnetic bead drying protection liquid is beneficial to application in micro-fluidic chips.

Description

technical field [0001] The invention relates to the technical field of biochemical reagents, in particular to a magnetic bead drying protection solution, drying magnetic beads and a preparation method thereof. Background technique [0002] Nucleic acid molecular biology technology is one of the commonly used research methods for pathogenic microorganism detection, species identification, species origin, diversity assessment and its kinship, and phylogenetic evolution. Whether high-quality nucleic acid can be extracted is the key in molecular biology experiments, and the sensitivity and specificity of the extraction method will also directly affect the success or failure of subsequent experiments. Therefore, nucleic acid extraction is one of the most critical methods in molecular biology. [0003] With the wide application of molecular biology, new requirements have been put forward for nucleic acid extraction technology. Efficiency, convenience, environmental protection, la...

AI Technical Summary

Problems solved by technology

[0009] The above protection solutions are all lyophilized protection solutions, which need to be freeze-dried for the preservation of immunomagnetic beads, while the hydroxyl magnetic beads will cause the aggregation of magnetic beads under freezing, drying and centrifugation conditions, which are not easy to resuspend and disperse, and Affects the chemical activity of functional groups on the surface of magnetic beads

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