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SNP molecular marker related to wheat stem rot resistance and application of SNP molecular marker

A technology of molecular markers and stalk rot, which is applied in the direction of recombinant DNA technology, microbial measurement/inspection, DNA/RNA fragments, etc., can solve the problem of unclear distribution of wheat varieties, so as to speed up the process of wheat disease-resistant breeding, The effect of saving experiment cost and practice, saving experiment cost

Active Publication Date: 2021-06-22
CHINA AGRI UNIV
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Problems solved by technology

However, these disease-resistant loci are all from foreign materials, and their distribution in my country's wheat varieties is still unclear.

Method used

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  • SNP molecular marker related to wheat stem rot resistance and application of SNP molecular marker
  • SNP molecular marker related to wheat stem rot resistance and application of SNP molecular marker
  • SNP molecular marker related to wheat stem rot resistance and application of SNP molecular marker

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Embodiment Construction

[0034] The following examples are used to illustrate the present invention, but are not intended to limit the scope of the present invention. Unless otherwise specified, the examples are all in accordance with conventional experimental conditions, such as Sambrook et al. Molecular Cloning Experiment Manual (Sambrook J & Russell DW, Molecular Cloning: a Laboratory Manual, 2001), or in accordance with the conditions suggested by the manufacturer's instructions. Example 1 Obtaining SNP Molecular Markers Related to Wheat Stem Rot Resistance

[0035] The present invention uses 55K single nucleotide polymorphism (SNP) sequences (chips purchased from Beijing Compson Biotechnology Co., Ltd.) to conduct genome-wide association studies on 358 Chinese winter wheat (including Sunco and Shan 253, which are resistant to susceptible control materials) (GWAS). Fusarium graminearum Wz2-8A 1×10 6 Soak 0.5-1cm germinated seeds in the spore / mL spore suspension, and plant them in plug trays. Whe...

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Abstract

The invention provides an SNP molecular marker related to wheat stem rot resistance and an application of the SNP molecular marker. Whole genome association analysis is carried out on a natural population composed of 358 Chinese wheat germplasm resources, and five SNP loci closely associated with stem rot resistance are found on a hexaploid wheat 5D chromosome long arm for the first time. KASP primers capable of being used for large-scale material screening are further developed for the SNP loci, and the close association degree of the developed molecular marker and disease resistance is verified in F2 and F2: 3 populations constructed by using an anti-infection material as a parent. The five SNP molecular markers provide an effective means for screening of wheat anti-FCR germplasm resources.

Description

technical field [0001] The invention relates to the fields of molecular biology and plant molecular breeding, in particular to SNP molecular markers related to wheat stem rot resistance and applications thereof. Background technique [0002] Stem rot of wheat is a worldwide soil-borne disease caused by the infection of Fusarium spp., due to its strong saprophytic nature, wide range of hosts, the main diseased part is the base of the stem, and the disease can occur during the whole growth period. With the application of farming measures such as returning straw to the field and saving water and fertilizer, the disease has seriously threatened the safe production of wheat in my country and has become a common new disease. [0003] Planting disease-resistant varieties is one of the most effective measures to prevent and control the occurrence and damage of stem rot. So far, no materials showing high resistance to the disease have been found internationally, and only some materia...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): C12Q1/6895C12N15/11
CPCC12Q1/6895C12Q2600/156C12Q2600/13
Inventor 马骏甄文超金京京段硕楠齐永志解超杰李伟闫素红
Owner CHINA AGRI UNIV
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