Hemispherical space compound eye-like somatic cell laser-induced fluorescence detector

Abstract

The invention discloses a hemispherical space compound eye-like somatic cell laser-induced fluorescence detector, which adopts a spherical compound eye structure formed by splicing an upper hemisphere compound eye patch body and a lower hemisphere compound eye patch body, and performs laser excitation and immunofluorescence receiving on each small eye by adopting an LIF light path formed by a small eye light path structure, an excitation light path structure and a fluorescence light path structure, so that the dynamic detection of the to-be-detected cells in each angle of a three-dimensional space can be realized, and the dynamic detection of the spatial distribution and change of specific antigen molecules in the cells can be realized. The detector structurally comprises an excitation light path structure, a fluorescence light path structure, a flow type sample injector and a spherical compound eye structure, wherein the spherical compound eye structure comprises an upper hemisphere compound eye patch body and a lower hemisphere compound eye patch body which can be closely spliced, the upper hemisphere compound eye patch body and the lower hemisphere compound eye patch body are provided with small eye light path structures, and the small eye light path structure is connected with the excitation light path structure and the fluorescence light path structure.

Description

technical field [0001] The invention relates to a laser-induced fluorescence detection system, in particular to a single-cell three-dimensional molecular body distribution detection system based on compound eye bionic optics. Background technique [0002] Cells are the basic units that constitute the structure of living organisms and realize the functions of life. From bacteria, cyanobacteria, protozoa composed of single cells, to multicellular organisms such as higher animals and plants, different types of cells with different functions and different numbers constitute the tissues, organs, systems, etc. of organisms from simple to complex life units, thus Realized the diversity of life activities. [0003] The cells are small in size, most typical cells are 1 to 30 microns in diameter, and individual larger cells can be as large as centimeters. The inner structure of the cell can be seen with the help of a light microscope, and an electron microscope is required for the f...

AI Technical Summary

Problems solved by technology

[0006] The defects of the above-mentioned existing immunofluorescence detection technology solutions are very significant. Cells can only be detected from a single fixed optical path angle. Therefore, it cannot meet the demand for dynamic detection of specific molecules inside the cell

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