PCR reaction device

Abstract

The invention relates to a PCR reaction device. The device comprises a PCR reaction main body, a display module and a power supply device, the PCR reaction main body comprises a nucleic acid extraction device, a nucleic acid purification device, an amplification reaction device and a shell, and the nucleic acid extraction device is used for cracking a sample to obtain a mixture; the nucleic acid purification device is used for independently separating nucleic acid from the mixture; the nucleic acid amplification reaction device is used for amplifying nucleic acid at a specified temperature by utilizing a temperature control unit, and the nucleic acid extraction device, the nucleic acid purification device and the nucleic acid amplification reaction device are all arranged in the shell; the display module is arranged at the end part of the PCR reaction main body and is used for displaying the working states of the nucleic acid extraction device, the nucleic acid purification device and the amplification reaction device; the power supply device is used for supplying power to the PCR reaction main body and the display module, and the power supply device is arranged on the right side of the PCR reaction main body. Therefore, the integration level of experimental equipment is high, the continuity of an experiment is high, and extraction, purification and amplification can be realized on one pipeline layer.

Description

technical field [0001] The invention relates to the field of biological detection, in particular to a PCR reaction device. Background technique [0002] The extraction, purification, and amplification of nucleic acid are routine operations in nucleic acid detection tests, and are also critical steps in the process of gene analysis. In the process of gene analysis, it is often necessary to separate specific nucleic acid fragments from mixed samples for subsequent PCR amplification. Therefore, the purification and recovery of nucleic acid directly affects the progress and results of the entire gene analysis process. [0003] The Chinese patent discloses a nucleic acid automatic detection device, which includes a fixed base and a main support plate vertically fixed on the fixed base, an automatic sample reaction mechanism and its fixed frame, a nucleic acid detection optical path structure and its supporting frame, and a controller; The automatic mechanism fixing frame is fixe...

AI Technical Summary

Problems solved by technology

[0004] In the above technical solutions, on the one hand, the detection device has high environmental requirements and low test accuracy. At the same time, the internal pipeline of the chip is not integrated, and it is difficult to mix and test the reaction solution as expected, and the extraction, purification and amplification of nucleic acid usually require Use different instruments to complete, and the operation is cumbersome

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