Molecular markers related to proliferation and metastasis of lung cancer and their application

Abstract

The invention provides molecular markers related to proliferation and metastasis of lung cancer and application thereof. The present invention adopts the gene editing technology CRISPR / Cas9 system. In human lung cancer cell lines, it is found that TMEM116 gene is closely related to the metastasis and proliferation of lung cancer. Lung cancer cell lines. On this basis, through in vitro and in vivo experiments in mice, it was found that the oncogenic ability and metastatic ability of lung cancer cells knocked down by TMEM116 were significantly reduced, the relationship between TMEM116 gene and lung cancer was clarified, and TMEM116 gene was found to be an important factor for the proliferation and metastasis of lung cancer. Related molecular markers are used in the diagnosis of lung cancer, and provide a new method for the diagnosis and treatment of lung cancer.

Description

technical field

[0001] The invention relates to the field of medical molecular biology, in particular to the technical field of lung cancer diagnosis, and in particular to molecular markers related to proliferation and metastasis of lung cancer and applications thereof. Background technique

[0002] Due to the stimulation of smoking, air pollution and some chemical factors, the normal lung cells of the human body are damaged, and gene mutations are prone to occur in the process of body damage repair, which in turn leads to the occurrence of tumors and cancers. Currently, lung cancer is also one of the most common malignancies, accounting for 18.4% of cancer deaths worldwide. Lung cancer is a highly complex tumor with a generally severe prognosis, with a 5-year survival rate of only 15%. In addition, under the promotion of metabolic changes and immune evasion, lung cancer cells can easily metastasize to other organs through blood vessels and lymphatic vessels, forming second...

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