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Cutting method for thornless zanthoxylum bungeanum tissue culture seedlings

A cutting method and tissue culture seedling technology are applied in the cutting field of thornless Zanthoxylum bungeanum tissue culture seedlings, which can solve the problems of low survival rate and low yield, and achieve the effects of simple breeding method, improved yield, and guaranteed quality and quantity of seedlings.

Inactive Publication Date: 2021-09-03
CHONGQING UNIV OF ARTS & SCI +1
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  • Abstract
  • Description
  • Claims
  • Application Information

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Problems solved by technology

To solve the problems of low survival rate and low yield of prickly prickly ash by grafting

Method used

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  • Cutting method for thornless zanthoxylum bungeanum tissue culture seedlings
  • Cutting method for thornless zanthoxylum bungeanum tissue culture seedlings

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Embodiment 1

[0036] A method for subculture of prickly prickly ash tissue culture seedlings, comprising the following steps:

[0037]S1. Primary culture: put the sprouted stems of prickly prickly ash in the culture medium, and culture them in a dark environment for 8-9 days, then place them under the condition of light intensity of 1000lux for 7-8 days, and then increase the light intensity to 2000lux Cultivate for 13 to 14 days until the sprouted stems of prickly prickly ash grow to a height of 3 to 4 cm, with 3 to 4 internodes and no less than 2 axillary buds. figure 1 shown;

[0038] S2. Cutting: place the sprouted stems of the prickly prickly ash through the primary culture in the aseptic inoculation tray on the ultra-clean workbench, and use sterilized tools to immerse the base of the sprouted stems of the prickly prickly ash into one end of the culture medium Remove the tissue to expose the fresh tissue, then cut the terminal buds into 1-2cm long stems with 2-4 leaves, and cut the r...

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Abstract

The invention discloses a subculture breeding method for thornless zanthoxylum bungeanum tissue culture seedlings, and belongs to the technical field of vegetative propagation of commercial crops. The invention provides a cutting method of thornless zanthoxylum bungeanum tissue culture seedlings, which comprises the following steps: placing thornless zanthoxylum bungeanum sprout stem sections subjected to primary culture in a sterile inoculation disc on an ultra-clean workbench, cutting off the base parts of the thornless zanthoxylum bungeanum sprout stem sections, namely the tissue at one end immersed into a culture medium, by using a sterile tool to expose fresh tissue, and cutting the fresh tissue into 1-3 leaf stem sections. By using the special cutting process, the generation number of subculture multiplication culture is greatly reduced, so that the purpose of rapidly propagating thornless zanthoxylum bungeanum seedlings is achieved, and the cutting method has a great practical value in propagation production of the hornless zanthoxylum bungeanum.

Description

[0001] This application is a divisional application of a Chinese patent application with the application number "202010136322.4", the title of the invention "a method for subculture of thornless Zanthoxylum bungeanum seedlings", and the filing date is 2020.03.02. technical field [0002] The invention belongs to the technical field of asexual propagation of economic crops, and in particular relates to a method for cutting tissue-cultured seedlings of prickly prickly ash. Background technique [0003] Zanthoxylum is a spice plant of Rutaceae Zanthoxylum Linn. It has a long history of cultivation in my country. It is not only a spice plant but also a traditional medicinal plant. Cultivated Zanthoxylum has high economic value and soil and water conservation benefits. Zanthoxylum bungeanum is distributed in the provinces of the Yellow River, Yangtze River, and Pearl River basins, as well as Yunnan, Guizhou, and Tibet in China. According to the type of commodity, it can be divided...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): A01H4/00
CPCA01H4/008A01H4/002
Inventor 陈泽雄唐宁娄娟许锋刘霞
Owner CHONGQING UNIV OF ARTS & SCI
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