Detection method of yeast mitosis recombination hotspot

A technology of mitosis and detection methods, applied in the field of synthetic biology, can solve the problems of insufficient research on yeast recombination hotspots and cold spots, screening of restricted recombination strains, etc.
CN113549682AActive Publication Date: 2021-10-26TIANJIN UNIV
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Patent Information

Authority / Receiving Office
CN Β· China
Patent Type
Applications(China)
Current Assignee / Owner
TIANJIN UNIV
Publication Date
2021-10-26

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Abstract

The invention relates to the technical field of synthetic biology, in particular to a detection method of yeast mitosis recombination hotspot. A PCRTag tag combination provided by the invention can be used for rapidly detecting eukaryotic genome mitosis recombination hotspots. By utilizing the tag combination, the mitosis recombination hotspot of the homologous chromosome can be quickly identified through a specific watermark tag, and a convenient method is provided for researching the eukaryotic mitosis recombination event.
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Description

Technical field

[0001] The present invention relates to the field of synthesis biological techniques, and more particularly to detecting methods of yeast mitotic recombinant hotspots. Background technique

[0002] The homologous recombination between the DNA molecules refers to a biological process between the DNA sequence undergo a double-strand break, recombinant repair, and chromosome separation process to achieve a novel DNA molecule. The homologous recombination can not only repair DNA's double-strand break (DSB), maintain genome stability, but also achieve cross-interchange of genetic information, generating genetic diversity of child cells, and accelerates biological evolution.

[0003] The homologous recombination contains two ways of decancing splitting recombination and mitotic recombination, in natural state, genetic substances in cells more inclined to minor reduction splitting recombination, and the incidence of mitofer recombination is lower than the reduction split...

Claims

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