27 results about "Streptomyces thioluteus" patented technology

The invention relates to a Zhongshengmycin production strain and application of the Zhongshengmycin production strain, the strain is streptomyces sp. ZS-2, the Zhongshengmycin production strain has good Zhongshengmycin production activity, and a fermentation product has higher bacteriostatic activity and a wider bacteriostatic range.

The application discloses a nitrogen acetylated carbazole alkaloid, and the structure is selected from one of the following structures: The application discloses a nitrogen acetylated carbazole alkaloid produced by fermentation of streptomyces J1074, and provides a biosynthetic preparation method, which is more green and environmental protection, and cost reduction compared with a chemical synthesis method; the compound ATC-A provided by the application shows significant antithrombotic activity in a thrombotic zebrafish model, which may be related to down-regulation of gene expression related to platelet activation and blood coagulation cascade, and has important development prospect and clinical application value.

This invention belongs to the field of biocatalysis technology and relates to a bifunctional enzyme for cysteine ​​desulfurization / desulfurization derived from marine Streptomyces and its applications. The amino acid sequence of the enzyme is shown in SEQ ID NO.1 of the sequence listing. This bifunctional enzyme can efficiently catalyze the conversion of cysteine ​​to alanine and pyruvate under mild conditions. The resulting alanine can be used as a food additive and pharmaceutical intermediate, while pyruvate has wide applications in the pharmaceutical and nutritional supplement fields. Therefore, this enzyme has good application prospects.

This invention relates to the fields of synthetic biology and metabolic engineering, specifically to a Streptomyces promoter selection marker and its application. The selection marker gene is *Streptomyces lilacinus*. Streptomyces lavendulae Glutamine indigo synthase gene derived from CGMCC 4.1386 idgS This invention screens endogenous promoters of *Streptomyces freundii* using heterologously expressed idgS genes; and constructs a detection system for endogenous promoters of *Streptomyces freundii* using this scheme, which can rapidly detect endogenous promoters of *Streptomyces freundii* and the strength of each promoter.

The present invention relates to a cytochrome P450 enzyme comprising the amino acid sequence set forth in SEQ ID NO: 3, or a variant thereof having an amino acid sequence having at least 95% identity thereto and having CYP450 activity. The cytochrome P450 enzyme provided herein was isolated from Streptomyces eurythermus NRRL 2539 and has a wide substrate range and high activity, and may be used to oxidate organic compounds.

The invention provides a method for improving the yield of thaxtomin A through a streptomyces scabies SCAB82261 gene. The method comprises the following steps: deleting the streptomyces scabies SCAB82261 gene through genetic engineering to obtain a thaxtomin A high-yield strain, and carrying out fermentation production; wherein the nucleotide sequence of the SCAB82261 gene is as shown in SEQ ID NO. 1, and the coded amino acid sequence is as shown in SEQ ID NO. 2. The invention has the following advantages: the thaxtomin A biosynthesis negative regulation gene SCAB82261 is screened, and the PaaK protein encoded by the thaxtomin A biosynthesis negative regulation gene SCAB82261 can catalyze the degradation of aromatic compounds such as phenylalanine; when the streptomyces scabies SCAB82261 gene is deleted, a high-yield strain of the thaxtomin A can be obtained, and a technical support is provided for improving the yield of industrial production of the thaxtomin A.

The application discloses a kind of high thermal stability glutamine transaminase mutant and its method of expression in streptomyces, belong to enzyme engineering field.The high thermal stability glutamine transaminase mutant provided by the application can efficiently catalyze casein crosslinking at high temperature, realizes the heterologous efficient expression of glutamine transaminase.Rational mutation is carried out to key amino acid site of glutamine transaminase, and the mutant strain with significantly improved thermal stability is obtained.Among them, the optimal mutant strain FRAPD-TGm2A3 has a half-life of up to 537.91 min at 60 DEG C.The mutant can still efficiently catalyze the crosslinking reaction of casein at high temperature of 85 DEG C.The present application not only lays a solid research foundation for the modification of thermal stability of glutamine transaminase, but also provides a generalizable strategy framework for the rational modification of other thermostable enzymes, and has important industrial application value and academic reference significance.

The invention provides a method for improving the yield of lincomycin by modifying a streptomyces lincomycin SLCG3615 gene, which comprises the following steps: knocking out a TetR family transcriptional regulation gene SLCG3615 in the streptomyces lincomycin through a genetic engineering way to obtain a lincomycin high-yield engineering strain, and fermenting the obtained strain to produce the lincomycin. Wherein the nucleotide sequence of the SLCG3615 gene is as shown in SEQ ID NO. 1. The invention also provides an application of the SLCG3615 gene knockout modified streptomyces lincomus. The lincomycin biosynthesis negative regulator SLCG3615 is screened in the research, the SLCG3615 gene copy on the chromosome of the streptomyces lincomycin is knocked out through a genetic engineering way, a lincomycin high-yield strain can be obtained, and a technical support is provided for improving the fermentation yield of the lincomycin in industrial production.

The application discloses streptomyces 891-DC with high aurovertin A yield and application thereof. The application uses CRISPR-Cas9 technology to first change an incomplete attB site sequence in the genome of streptomyces 891 into a complete attB site sequence, promote a plasmid containing a target gene chry, an integrase phiC31 gene and an attP site, and improve the integration rate of the target gene through specific combination of the attP site and the attB site on the genome. Secondly, a promoter capable of obviously improving the yield is screened to express the aurovertin biosynthesis gene cluster, so that the more stable and higher-yield streptomyces 891-DC is obtained. The aurovertin A shake flask fermentation yield is 1411.23+ / -29 mg / L, which is 60% higher than that of the original strain, and the genetic stability is good, thereby laying a technical foundation for industrial production of aurovertin.

The application discloses application of a phzB-G gene in synthesis of phenazine-1,6-dicarboxylic acid. The application is that the phzB-G gene is connected with an integrated plasmid, and then integrated into a Streptomyces genome to construct a Streptomyces engineering strain, which is used for synthesizing phenazine-1,6-dicarboxylic acid. The Streptomyces engineering strain constructed by the application can stably and specifically synthesize phenazine-1,6-dicarboxylic acid, and further through screening of different promoters, the synthesis yield can be greatly improved.

This invention belongs to the field of gene editing technology and discloses a high-efficiency CRISPR / Cas9n editing vector suitable for *Streptomyces freundii* and its applications. The aforementioned editing vector includes P... kasO * indicates the sgRNA expression cassette of the promoter and the thioseriin-induced promoter P. tipA The HF-Cas9n expression cassette regulates expression. The gene editing vector provided by this invention enables precise and efficient editing of the *Streptomyces freundii* genome, with a success rate of up to 100%, providing a reliable tool for efficiently constructing engineered *Streptomyces freundii* strains to improve the industrial production of *Streptomyces freundii* cell products.

The invention relates to an application of 2-methyl-4 (3H)-quinazolinone in preparation of an anti-angiogenesis medicine. The invention discloses the application of the 2-methyl-4 (3H)-quinazolinone compound as an angiogenesis inhibitor in anti-angiogenesis drugs for the first time, and the 2-methyl-4 (3H)-quinazolinone compound has a remarkable inhibition effect on internode blood vessels and dorsal longitudinal anastomosis angiogenesis of a Tg (flk: mCherry) fluorescent transgenic zebrafish strain. Meanwhile, the growth of the zebrafish embryos of the strain is not obviously influenced, and the toxicity is extremely low. The compound not only can be extracted from streptomyces, but also can be obtained through an artificial synthesis process, has a reliable and stable source, and has a good application prospect in the field of anti-angiogenesis drugs.

The application discloses a kind of biochar-based multiple-effect soil conditioner and preparation method thereof, belong to soil remediation technical field.It is made of the following weight parts of raw materials: modified biochar 100-120 parts, microbial inoculum 10-20 parts, modified red mud 20-30 parts, diatomite 20-30 parts, poultry manure 80-100 parts, activator 10-20 parts;The microbial inoculum is Brevibacillus laterosporus, Trichoderma koningii, Streptomyces celluloxys and is composed according to the mass ratio of 1:1:1.The biochar-based multiple-effect soil conditioner prepared by the application can effectively improve the physical and chemical properties of soil through the synergistic effect of multiple components, such as increasing the pH value of acidic soil, increasing the organic matter content of soil, improving soil structure, increasing soil enzyme activity, etc., thereby significantly improving the fertility and health level of soil, which helps to promote the growth and development of plants and improve the yield and quality of crops.At the same time, the modified biochar and modified red mud are used in the application to improve the aeration and water permeability of soil, reduce the compactness of soil, improve the physical properties of soil, and also can combine with heavy metal ions in soil, reduce the biological availability of heavy metals, reduce the pollution of heavy metals to soil and crops, have excellent multiple soil improvement effect, and have wide market application prospect.

This invention provides a method for modifying Streptomyces lincosinate. SLCG_1540 Methods to increase lincomycin production through gene therapy involve knocking out LacI family transcriptional regulatory genes in Streptomyces lincomyces via genetic engineering. SLCG_1540 A high-yield engineered strain of lincomycin was obtained, and the obtained strain was used to produce lincomycin through fermentation; among which, SLCG_1540 The nucleotide sequence of the gene is shown in SEQ ID NO.1. This invention also provides the above-mentioned... SLCG_1540 Applications of gene knockout modified *Streptomyces lincomycin*. The advantages of this invention are: the screening of a negative regulator of lincomycin biosynthesis, SLCG_1540, and experimental findings that gene knockout of *Streptomyces lincomycin* chromosomes via genetic engineering... SLCG_1540 This invention provides technical support for increasing lincomycin fermentation yield in industrial production by generating genes that can produce high-yield strains of lincomycin.

The invention relates to a biosynthesis gene cluster of a tropolone compound, namely isatropolones, and a bacterial strain capable of realizing directional high yield. The gene cluster comprises an isaF gene, an isaJ gene and an isaS gene; the sequence of the isaF gene is as shown in SEQ ID NO.1, the sequence of the isaJ gene is as shown in SEQ ID NO.3, and the sequence of the isaS gene is as shown in SEQ ID NO.5. The strain capable of realizing the directional high yield is (1) a strain capable of realizing the directional high yield of isatropolone A, namely a strain which takes streptomyces sp.CGMCC (China General Microbiological Culture Collection Center) No.15540 as a host and is used for blocking an isaJ gene of the host or blocking an isaS gene of the host and is also used for realizing the overexpression of isaF at the same time, and the strain capable of realizing the directional high yield of the isatropolone A is a strain capable of realizing the directional high yield of the isatropolone A. And (2) the strain of the oriented high-yield isatropolone C is a strain which takes streptomyces sp.CGMCC (China General Microbiological Culture Collection Center) No.15540 as a host and is used for overexpressing the genes of isaF and isaS, or a strain which is used for overexpressing the genes of isaJ and isaS, or a strain which is used for overexpressing the genes of isaF, isaJ and isaS, or a strain which is used for overexpressing the genes of isaF, isaJ and isaS.

The invention provides a high-activity glutamate oxidase mutant and application thereof in production of alpha-ketoglutaric acid.The high-activity glutamate oxidase mutant is obtained by conducting site-specific mutagenesis on wild-type glutamate oxidase derived from streptomyces moosgenus, specifically, leucine L at the 80th site is mutated into valine V, and the valine V is mutated into valine. In addition, aspartic acid D at the 596th site is mutated into glutamic acid E, and then a fragment is inserted into a pET21a expression vector to transform escherichia coli BL21 (DE3). Compared with wild-type glutamate oxidase, the glutamate oxidase mutant constructed by the invention has the advantages that the molar conversion rate of a substrate is increased by 22.4-23.8%, and compared with other glutamate oxidase mutants, the molar conversion rate of the substrate is increased by 0.45-1.54%. The glutamate oxidase mutant constructed by the invention effectively improves the conversion efficiency of glutamate oxidase, effectively solves the technical bottleneck of insufficient conversion efficiency and the like in the existing alpha-ketoglutaric acid (a-KG) production process, and has important application value for promoting the industrial production of alpha-ketoglutaric acid.

This invention discloses a heparin sulfate derivative derived from donkey-hide gelatin with inhibitory activity against the SARS-CoV-2 spike protein and its preparation method. The heparin sulfate derivative is prepared by first enzymatically hydrolyzing a donkey-hide gelatin solution with streptomycin, followed by separation and purification using DEAE anion exchange chromatography to obtain donkey-hide gelatin glycosaminoglycans. These glycosaminoglycans are then enzymatically hydrolyzed again using chondroitin sulfate ABC enzyme, followed by separation and purification using DEAE anion exchange chromatography. The derivative has an average molecular weight of 13.2 kDa and is composed of disaccharide units linked by hexuronic acid and glucosamine via 1-4 glycosidic bonds. The total sulfation degrees at the C-2-O position of hexuronic acid, the C-6-O position of glucosamine, and the N-position are 28.2%, 44.3%, and 38.9%, respectively. This heparin sulfate derivative exhibits competitive inhibition of the binding activity between the SARS-CoV-2 spike protein and heparin, enabling effective prevention of early-stage SARS-CoV-2 infection and laying an important foundation for developing new clinical applications of donkey-hide gelatin.

The invention relates to the technical field of genetic engineering, and provides a method for improving the yield of lincomycin by modifying a streptomyces lincolensis SLCG3264 gene, a MarR family transcriptional regulation gene SLCG3264 in streptomyces lincolensis is knocked out through a genetic engineering way to obtain a lincomycin high-yield strain, and the obtained strain is used for fermentation production of lincomycin. Wherein the nucleotide sequence of the SLCG3264 gene is as shown in SEQ ID NO. 1. The invention also provides an application of the SLCG3264 gene knockout modified streptomyces lincomus. According to the invention, the lincomycin biosynthesis negative regulator SLCG3264 is screened, and the SLCG3264 gene on the chromosome of the streptomyces lincomycin is knocked out through a genetic engineering way, so that a lincomycin high-yield strain can be obtained, and a technical support is provided for improving the fermentation yield of the lincomycin in industrial production.

This invention provides a method for modifying Streptomyces lincosinate. SLCG_3615 A method to increase lincomycin production through gene therapy involves knocking out the TetR family transcriptional regulatory genes in Streptomyces lincomyces via genetic engineering. SLCG_3615 A high-yield engineered strain of lincomycin was obtained, and the obtained strain was used to produce lincomycin through fermentation; among which, SLCG_3615 The nucleotide sequence of the gene is shown in SEQ ID NO.1. This invention also provides... SLCG_3615 Applications of gene knockout modified *Streptomyces lincomycin*. In this study, the negative regulator of lincomycin biosynthesis, SLCG_3615, was screened and knocked out on the chromosome of *Streptomyces lincomycin* via genetic engineering. SLCG_3615 SLCG_3615 Gene copying can yield high-yield strains of lincomycin, providing technical support for increasing the fermentation yield of lincomycin in industrial production.

The invention provides a method for improving the yield of lincomycin by modifying a streptomyces lincomycin SLCG1540 gene, which comprises the following steps: knocking out a LacI family transcriptional regulation gene SLCG1540 in the streptomyces lincomycin through a genetic engineering way to obtain a lincomycin high-yield engineering strain, and fermenting the obtained strain to produce the lincomycin. Wherein the nucleotide sequence of the SLCG1540 gene is as shown in SEQ ID NO. 1. The invention also provides an application of the SLCG1540 gene knockout modified streptomyces lincomus. The lincomycin biosynthesis negative regulator SLCG1540 has the advantages that the lincomycin biosynthesis negative regulator SLCG1540 is screened out, and it is found through experiments that a lincomycin high-yield strain can be obtained by deleting the SLCG1540 gene on a streptomyces lincomycin chromosome through a genetic engineering way; the invention provides technical support for increasing the fermentation yield of lincomycin in industrial production.

This invention relates to a streptomycin-producing strain and its application, wherein the strain is Streptomyces. Streptomyces sp. ZS-2 has good activity in producing kasugamycin, and its fermentation products have higher antibacterial activity and a wider antibacterial range.

This invention relates to a streptomycin-producing strain and its application, wherein the strain is Streptomyces. Streptomyces sp. ZS-1 has good activity in producing kasugamycin, and its fermentation products have higher antibacterial activity and a wider antibacterial range.

The application discloses a regulatory gene of streptomyces roseosporus for producing daptomycin, wherein the nucleotide sequence is shown in SEQ ID NO:1, or the nucleotide sequence is the same as the nucleotide sequence encoding the same protein as shown in SEQ ID NO:1. The application solves the problems of high workload and high blindness of traditional streptomyces breeding by using a global regulatory factor CRP, and obtains a daptomycin high-yield strain cultivated by genetic engineering, which has a wide application prospect. The method is used for modifying the starting bacteria streptomyces roseosporus, and it is accidentally found that the CRP gene can improve the utilization rate of capric acid of the streptomyces roseosporus in the fermentation process and improve the tolerance of the streptomyces roseosporus to the capric acid.

The invention discloses streptomyces capable of producing aureomycin A at high yield and construction and application of the streptomyces. Through a promoter engineering strategy, a strong promoter Rpsl is added before a gene cluster is biosynthesized by aureomycin, a heterologous expression engineering strain M145-Rpsl-chry capable of producing aureomycin A at high yield is constructed, the genetic stability of the strain is good, plasmids are not lost after the strain is transferred to five generations, and the strain has a good application prospect. The method can be applied to industrial production of the aureomycin A. According to the method, the yield of the aureomycin A in the heterologous host streptomyces coelicolor is effectively increased, and the problem of low yield of heterologous expression aureomycin A is solved.

The invention relates to the technical field of genetic engineering, and provides a method for improving the yield of thaxtomin A through a streptomyces scabies SCAB78491 gene. The method comprises the following steps: deleting the SCAB78491 gene of the streptomyces scabies through a genetic engineering way, constructing a thaxtomin A high-yield strain, and carrying out fermentation production; the sequence of the SCAB78491 gene is as shown in SEQ ID NO. 1, and the coded amino acid sequence of the SCAB78491 gene is as shown in SEQ ID NO. 2. The SCAB78491 gene in streptomyces scabies is determined as the thaxtomin A biosynthesis negative regulation gene for the first time through screening and identification, and the SCAB78491 gene in the streptomyces scabies is directionally deleted to obtain a thaxtomin A high-yield engineering strain, so that the yield of the thaxtomin A is effectively increased, and a technical support is provided for industrial fermentation optimization.

The invention discloses recombinant streptomyces capable of producing terpenes at high yield and application of the recombinant streptomyces, and belongs to the technical field of microorganisms. According to the invention, a complete MVA approach is heterologously introduced into streptomyces for the first time, MVK / DPMD / PMK / IDI / HMG-CoA / HMGR / AACS key enzyme genes are amplified through PCR, an expression vector containing a kasOp promoter is constructed, and a recombinant strain is obtained through conjugational transfer. By taking lycopene as a visual marker, fermentation verification shows that the terpene yield of the recombinant strain is increased by about 50% compared with that of a control group. According to the invention, promoter activation and metabolic pathway optimization are combined, the limitation of single regulation is broken, and an efficient chassis is provided for synthesis of terpenoid derivatives.

The invention relates to morphologically optimized recombinant streptomyces nodosus for producing amphotericin B, and a construction method and application thereof. The cell morphology of the streptomyces nodosus is changed by overexpressing a galactose oxidase protein gene GlxA, so that the yield of the amphotericin B is increased, the viscosity of fermentation liquor is reduced, and the yield of the amphotericin B is increased. And the purposes of energy conservation and consumption reduction in the production process are finally achieved by changing the growth state of the strain. Starting from the cell morphology level of streptomyces nodosus, cell morphology related genes are modified, the viscosity of fermentation liquor is reduced, the fermentation energy consumption is reduced, and the yield of amphotericin B is increased. By adopting the strain fermentation method disclosed by the invention, the shake flask unit of amphotericin B can reach 6079.12 mg / L or above, which is improved by 8.12% compared with that of a control strain. A new thought is provided for industrial strain modification.