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Salinomycin strain

A salinomycin and strain technology, applied in bacteria, microorganisms, biochemical equipment and methods, etc., can solve the problems of high negative mutation rate and resistance saturation, strain viability, weakened sporulation, and lack of space. Good stability, great economic value, and the effect of increasing production

Inactive Publication Date: 2014-09-03
ZHEJIANG SHENGHUA BIOK BIOLOGY
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

For a long time, traditional breeding methods such as ultraviolet mutagenesis and LiCl mutagenesis have been used in the random breeding technology for the improvement of Streptomyces albus strains, and have achieved certain results. However, long-term use of these mutagenesis When the same strain is treated with a variable agent, due to the non-directionality of mutations and the accumulation of mutations, the viability and spore production of the strain will be significantly weakened while obtaining high yield, and multiple mutagenesis often leads to a higher negative mutation rate. and resistance saturation, leaving insufficient room for strain improvement

Method used

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Examples

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Embodiment 1

[0030] (1) Use normal temperature and pressure plasma (ARTP) to mutagenize the starting strain

[0031] Preparation of starting strain F 2 -1 # The spore suspension was blown dry under sterile wind, and then placed under the ARTP mutagenesis system for plasma irradiation for 10 minutes. The samples were washed with physiological saline, spread on plate medium after serial dilution, and incubated in the dark for 5 days.

[0032] (2) High-throughput primary screening of mutant strains

[0033] Pick the single-colony mutant strain obtained in step (1) and inoculate two 96-well plates with 6ml of solid medium and 0.3ml of liquid fermentation medium with a sterile toothpick at the same time. The former is incubated at 25°C for 8 days, The latter were cultured at 25°C, 90% humidity, and 200 r / min for 8 days.

[0034] (3) High-throughput screening of mutant strains

[0035] The solid culture of the high-yielding mutant strain obtained in step (2) was spotted with a sterile tooth...

Embodiment 2

[0041] (1) Use normal temperature and pressure plasma (ARTP) to mutagenize the starting strain

[0042] Preparation of starting strain F 2 -1 # The spore suspension was blown dry under sterile wind, and then placed in the ARTP mutagenesis system for plasma irradiation for 1 min. The samples were washed with physiological saline, spread on plate medium after serial dilution, and incubated in the dark for 8 days.

[0043] (2) High-throughput primary screening of mutant strains

[0044]Pick the single-colony mutant strain obtained in step (1) and inoculate two 24-well plates with 1ml of solid medium and 1.5ml of liquid fermentation medium with a sterile toothpick at the same time. The latter was cultured at 37°C, humidity 50%, 300r / min for 4 days.

[0045] (3) High-throughput screening of mutant strains

[0046] The solid culture of the high-yielding mutant strain obtained in step (2) was spotted with a sterile toothpick in a 24-well deep plate containing 5ml of seed medium,...

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Abstract

The invention discloses a salinomycin strain with the class name of streptomycesalbums S-610-11, and the preservation number is CCTCCM2012492. The salinomycin high-yielding strain streptomycesalbums S-610-11 is obtained by combination of plasma mutagenesis at normal temperature under normal pressure with high-throughput synergy screening method, the content of salinomycin can be greatly improved, and the stability is good, after 5 times of continuous passages of the strain, and the salinomycin titer has no obvious change. By use of shake flask fermentation, the yield of salinomycin reaches 56000u / ml, and is improved by 70% compared with that of original strain F2-1#. The salinomycin strain is fully consistent with mutagenesis and screening requirements of industrialization salinomycin production strains, and can be applied in industrial fermentation production, and has great economic value.

Description

technical field [0001] The invention relates to the field of screening and application of industrial strains, in particular to a salinomycin strain. Background technique [0002] Salinomycin is one of the main veterinary antibiotics produced, used and exported in my country. It can effectively kill various coccidia in poultry and is widely used in feed additives. It is an ideal anticoccidiostat and feed additive. It is a group of Streptomyces albicans ( Streptomyces albums ) produced monocarboxylic acid polyether is a special antibiotic for animals, which has a strong inhibitory and killing effect on most Gram-positive bacteria (including mycobacteria and some filamentous fungi) and various coccidia. Salinomycin is an anticoccidial agent approved by the US FDA and has been widely used in countries all over the world. [0003] Salinomycin is a typical ionophore antibiotic, it has positive effects on cations in cells, especially K + 、Na + , Rb + The affinity of cations is...

Claims

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Application Information

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IPC IPC(8): C12N1/20C12R1/47
Inventor 贾永峰蔡群芳吴黎诚熊莉陈中兵梁剑光徐顺清唐俊黄海涛
Owner ZHEJIANG SHENGHUA BIOK BIOLOGY
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