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Actinomycetes strain and application thereof in preparation of aromatic hydroxylamine

A technology of aromatic hydroxylamine and actinomycetes, applied in the direction of bacteria, microorganism-based methods, biochemical equipment and methods, etc., can solve the problems of poor reproducibility

Inactive Publication Date: 2010-06-16
EAST CHINA UNIV OF SCI & TECH +1
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

Although baker's yeast and plant cells have high catalytic activity and selectivity, there are still big problems in industrial mass production of baker's yeast and plant cells, and the reproducibility is poor

Method used

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  • Actinomycetes strain and application thereof in preparation of aromatic hydroxylamine
  • Actinomycetes strain and application thereof in preparation of aromatic hydroxylamine
  • Actinomycetes strain and application thereof in preparation of aromatic hydroxylamine

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0041] Synthesis of 4-Nitrophenylhydroxylamine

[0042] CGMCC No.2725 strain was cultured on a slant and the fermented liquid was centrifuged at 8000rpm for 15 minutes, and its supernatant (mycelium) was resuspended with a buffer (phosphate buffer) with a pH value of 7.5, and then 5g / L ("L" here refers to the volume of the buffer) of glucose to obtain a bacterial suspension.

[0043] Among them: Slant Medium: Soluble Starch 2%, KNO 3 0.1wt%, NaCl 0.05wt%, K 2 HPO 4 ·3H 2 O 0.05wt%, MgSO 4 ·7H 2 O 0.05wt%, FeSO 4 ·7H 2 O 0.001wt%, agar 1.8wt%, the balance is water; the pH value of the slant culture medium is 7.2.

[0044] The culture temperature on the slant was 28°C, and the culture time was 7 days.

[0045] Get above-mentioned bacterium suspension 6ml and 14ml buffer (phosphate buffer, pH value is 7.5) and the dimethyl sulfoxide (DMSO) solution containing 10mg 1,4-dinitrobenzene composition reaction solution, and make 1 , The solubility of 4-nitrobenzene in the re...

Embodiment 2

[0050] Synthesis of 2-Nitrophenylhydroxylamine

[0051] Except that 1,2-dinitrobenzene is used to replace 1,4-dinitrobenzene in Example 1, and the solubility of the "substrate" in the reaction solution is controlled at 900mg / (l is the total volume of the reaction solution) , other conditions (comprising test conditions) are all the same as described in Example 1. The results obtained are shown in Table 1.

[0052] Retention time against hydroxylamine standard: 2a, 20.0 minutes; 2c, 15.9 minutes; 2d, 19.0 minutes.

Embodiment 3

[0054] Synthesis of 3-Nitrophenylhydroxylamine

[0055] Except that 1,3-dinitrobenzene was used instead of 1,4-dinitrobenzene in Example 1, other conditions (including test conditions) were the same as those described in Example 1. The results obtained are shown in Table 1.

[0056] Retention time against hydroxylamine standard: 3a, 22.3 minutes; 3c, 13.1 minutes; 3d, 14.2 minutes.

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PUM

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Abstract

The invention relates to an actinomycetes (Streptomyces thioluteus) strain CGMCC No. 2725 and application thereof in the preparation of aromatic hydroxylamine. The strain CGMCC No. 2725 is easy to obtain and culture, and has a complete coenzyme regeneration system exists in a cell body of the strain, the spontaneous cyclic regeneration of coenzyme can be performed only by adding an appropriate quantity of auxiliary substrates (such as glucose or ethanol and the like) without additionally adding expensive reduced coenzyme. In addition, the use of intact cells can leave out the process of extracting and purifying enzyme, so the strain is more suitable for large-scale commercial application.

Description

technical field [0001] The invention relates to a strain of actinomyces (Streptomyces thioluteus) and its application. Background technique [0002] Aromatic hydroxylamines are a class of important compounds, they are key synthetic intermediates (Tetrahedron Lett, 2003, 44:849-851) and life active compounds (Bioorg.Med.Chem., 2000,8:405-851) of many fine chemicals. 412). In addition, aromatic hydroxylamine itself also has high pharmacological and physiological activities (Biochem. Pharmacol, 1991, 42: 1691-1697). [0003] So far, known methods for preparing aromatic hydroxylamines include the following: [0004] (1) Chemical preparation method [0005] The "traditional" chemical reduction method mainly achieves the purpose of preparing aromatic hydroxylamines by catalytic transfer hydrogenation or metal reduction of corresponding nitro compounds (J.Chem.Soc., Chem.Commun., 1986, 1655-1656.). However, the selectivity of these "traditional" reduction methods is relatively ...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): C12N1/20C12P13/00C12R1/465
Inventor 徐晓勇钱旭红张琳琳陶黎明旷文丰李忠徐文平
Owner EAST CHINA UNIV OF SCI & TECH
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