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Streptomyces thiogamboges St-79 and application thereof

A technology of Streptomyces thioluteus, st-79, applied in the direction of application, bacteria, chemicals for biological control, etc., can solve the problems of chemical control limitations, hazards, etc.

Active Publication Date: 2021-08-10
ZHEJIANG NORMAL UNIVERSITY
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

At present, the control method for rice bacterial blight is mainly chemical control, but chemical control has obvious limitations and hazards, and there are currently no particularly effective pesticides that can be applied to the control of bacterial diseases

Method used

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  • Streptomyces thiogamboges St-79 and application thereof
  • Streptomyces thiogamboges St-79 and application thereof
  • Streptomyces thiogamboges St-79 and application thereof

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0030] Embodiment 1, screening and identification of Streptomyces thioluteus St-79 bacterial strain

[0031] 1 strain

[0032] (1) Actinomycetes strains: isolate, purify, screen and identify from soils in different habitats, and preserve actinomycetes strains.

[0033] (2) Rice bacterial blight (Xanthomonas oryzae pv. oryzae, Xoo, hereinafter abbreviated as Xoo) can be preserved by our laboratory.

[0034] 2 media

[0035] (1) Gaoshi No. 1 solid medium: soluble starch 20g, KNO 3 1g, K 2 HPO 4 0.5g, MgSO 4 ·7H 2 O0.5g, NaCl 0.5g, FeSO 4 ·7H 2 O 0.01g, agar 20g, water 1000mL, pH 7.4. For the isolation, purification and identification of actinomycete strains.

[0036](2) Gaoshi No. 1 liquid medium: no agar is added, and the rest of the formula is the same as (1), and it is used for the liquid fermentation culture of actinomycete strains.

[0037] (3) NA medium: beef extract 3g, peptone 5g, NaCl 5g, agar 20g, water 1000mL, pH 7.2. For the cultivation of Xoo bacteria....

Embodiment 2

[0080] Embodiment 2, Streptomyces thioluteus (Streptomyces thioluteus) St-79 bacterial strain antimicrobial spectrum determination

[0081] 1 strain

[0082] (1) Streptomyces thioluteus St-79 strain.

[0083] (2) Four representative plant pathogenic bacteria: such as Xanthomonas oryzaepv.oryzicola, Xanthomonas campestris pv. campestris, and Pseudomonas syringae pv. tomato), soybean bacterial spot disease (Xanthomonasaxonopodis pv.glycines) 4 kinds of representative plant pathogenic bacteria (see Table 3), used for the antibacterial spectrum determination of St-79 strain fermentation filtrate.

[0084] 2 media

[0085] (1) NA liquid medium: beef extract 3g, peptone 5g, NaCl 5g, water 1000mL, pH 7.2 (NA solid medium plus agar 20g). For the cultivation of plant pathogenic bacteria.

[0086] (2) Gaoshi No. 1 liquid medium: soluble starch 20g, KNO 3 1g, K 2 HPO 4 0.5g, MgSO 4 ·7H 2 O0.5g, NaCl 0.5g, FeSO 4 ·7H 2 O 0.01g, water 1000mL, pH 7.2~7.4. Used for seed liquid an...

Embodiment 3

[0099] Embodiment 3, Streptomyces St-79 bacterial strain fermented filtrate is to the preventive effect research of rice bacterial blight

[0100] 1 Germs and rice varieties

[0101] (1) Actinomycetes used for control effect: Streptomyces thioluteus St-79 strain.

[0102] (2) Pathogenic bacteria: rice bacterial blight (Xanthomonas oryzae pv.oryzae, Xoo)

[0103] (3) Rice variety: Yongyou 1540 variety susceptible to rice bacterial blight was selected.

[0104] 2 media

[0105] (1) Gaoshi No. 1 liquid medium: soluble starch 20g, KNO 3 1g, K 2 HPO 4 0.5g, MgSO 4 ·7H 2 O0.5g, NaCl 0.5g, FeSO 4 ·7H 2 O 0.01g, water 1000mL, pH 7.4 (solid medium plus agar 20g), used for the preparation of the culture fermentation filtrate of the St-79 bacterial strain.

[0106] (2) NA solid medium: beef extract 3g, peptone 5g, NaCl 5g, agar 20g, water 1000mL, pH 7.2 (liquid medium without agar). For the cultivation of Xoo bacteria.

[0107] 3 Experimental methods

[0108] 3.1 Rice cult...

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PUM

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Abstract

The invention discloses streptomyces thioluteus St-79, and the preservation number of the streptomyces thioluteus St-79 is CCTCC (China Center for Type Culture Collection) NO: M 2020962. The application of the streptomyces thioluteus St-79 strain is as follows: the germination of rice seeds is promoted, the bacterial blight of rice is prevented and treated, and the pathogenic bacteria of plants are inhibited.

Description

technical field [0001] The invention belongs to the field of microbial biological control, and relates to Streptomyces thioluteus St-79 strain antagonizing rice white leaf pathogen and its application in promoting rice seed germination. Background technique [0002] Rice bacterial blight first occurred in Japan. Due to the spread of rice seeds, the disease has occurred in all major rice regions in Asia, among which Japan, China, India and other places are more serious. At present, the areas affected by bacterial blight in China are mainly concentrated in rice production areas such as East China and Central China, while it is rare in southwest and northwest regions. The pathogen is transmitted by water and invades rice through small holes or wounds on the leaves, and generally causes the epidemic of the disease in years with more typhoons and heavy rains or floods. [0003] Rice bacterial blight is caused by the bacterium Xanthomonas oryzae pv.oryzae, Xoo. The typical sympto...

Claims

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Application Information

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IPC IPC(8): C12N1/20A01N63/28A01P21/00A01P1/00C12R1/465
CPCA01N63/28
Inventor 蒋冬花马静静潘妍妍杨孙玉悦王炫栋
Owner ZHEJIANG NORMAL UNIVERSITY
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