Application of deoxyribonuclease I to preparation of medicine for treating and preventing traffic hydrocephalus

A deoxyribonucleic acid, communicating hydrocephalus technology, applied in the field of biomedicine, can solve the problem of no deoxyribonuclease I treatment, etc., achieve the effect of reducing the degree of ventricle expansion and improving neurocognitive function

Pending Publication Date: 2021-10-29
THE FIRST AFFILIATED HOSPITAL OF ARMY MEDICAL UNIV
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  • Abstract
  • Description
  • Claims
  • Application Information

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  • Application of deoxyribonuclease I to preparation of medicine for treating and preventing traffic hydrocephalus
  • Application of deoxyribonuclease I to preparation of medicine for treating and preventing traffic hydrocephalus
  • Application of deoxyribonuclease I to preparation of medicine for treating and preventing traffic hydrocephalus

Examples

Experimental program
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Embodiment 1

[0023] The increase of cell-free DNA in cerebrospinal fluid is an important pathological mechanism of communicating hydrocephalus

[0024] Establishment of a model of communicating hydrocephalus induced by kaolin in rats. Adult male SD rats (250-300g, purchased from the Experimental Animal Center of the Army Military Medical University) were anesthetized by intraperitoneal injection of pentobarbital (40mg / kg). skull base. Prepare 25% (250 mg / mL) sterile kaolin (Sigma-Aldrich) suspension with normal saline, and inject 30 μL of the prepared kaolin suspension into the basal pool with a syringe along the gap between the bottom of the skull and the first cervical vertebra to induce traffic Sexual hydrocephalus. Set up a blank control group (inject 30 μL of normal saline into the basal cistern, n=6), a normal saline group (administer 5 μL of normal saline through the lateral ventricle after modeling, n=6), a DNase1 group (administer 5 μL of normal saline through the lateral ventri...

Embodiment 2

[0027] Effect of deoxyribonuclease I on ventriculomegaly in communicating hydrocephalus

[0028] 28 days after the experimental animals were modeled, a small animal 7.0T cranial MRI examination was performed.

[0029] Experimental results such as figure 2 As shown in middle A, the MRI T2 sequence images showed that compared with the blank control group, the lateral ventricle of the animals in the normal saline group was significantly dilated, and the degree of dilation of the lateral ventricles of the animals in the DNase1 group treated with DNase I was significantly less than that of the normal saline group.

[0030] Further, the volume of the lateral ventricle of each group of animals was measured and counted, and the results were as follows: figure 2 As shown in middle B, compared with the blank control group, the volume of the lateral ventricle of the animals in the normal saline group was significantly increased (P<0.0001), while the volume of the lateral ventricle of ...

Embodiment 3

[0032] Effects of deoxyribonuclease I on neurocognitive function in rats with communicating hydrocephalus

[0033] The neurocognitive function of the animals in each group was evaluated by using the Water Morris Maze experiment on the 23rd day after the model was established. The experimental animals were placed in a circular pool (200 cm in diameter) with a water depth of 50 cm, and the pool water was dyed black with ink. The surface of the pool is divided into four quadrants on average, and a platform with a diameter of 6 cm is placed in one of the quadrants, and the platform is submerged 1.5 cm underwater. The experiment started from the 23rd to 27th day after modeling as the training stage. The experimental animals were placed into the water from different quadrants, and the animals were allowed to find the underwater platform within 180s. If they still did not find the underwater platform after 180s, they were placed on the platform for 60s. . After 5 days of training, ...

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Abstract

The invention discloses an application of deoxyribonuclease I to preparation of a medicine for treating and preventing traffic hydrocephalus. The application is characterized in that the deoxyribonuclease I is used as an effective component and is used for degrading free DNA (deoxyribonucleic acid) in cerebrospinal fluid after intracranial hemorrhage or intracranial infectious diseases, so that the traffic hydrocephalus is treated and prevented; and the neurocognitive function is improved by relieving ventricular dilation caused by traffic hydrocephalus. Animal experiments prove that a kaolin-induced rat traffic hydrocephalus model is used as a research carrier. The level of the free DNA in cerebrospinal fluid can be remarkably reduced by injecting the deoxyribonuclease I into the lateral ventricle, and the nuclear magnetic resonance result shows that the deoxyribonuclease I can remarkably reduce the degree of ventricle expansion after 28 days. Through further application of a water morris maze experiment, it is found that the deoxyribonuclease I can obviously improve the neurocognitive function of an experimental animal.

Description

technical field [0001] The invention belongs to the technical field of biomedicine and relates to the application of deoxyribonuclease I in the preparation of medicines for treating and preventing communicating hydrocephalus. Background technique [0002] Communicating hydrocephalus is one of the most serious complications of intracranial hemorrhage and intracranial infectious diseases, and often indicates a poor prognosis. At present, the most commonly used method for clinical treatment of communicating hydrocephalus is cerebrospinal fluid shunt surgery. However, shunt surgery is less effective because of the high incidence of complications such as shunt blockage or infection. Therefore, there is an urgent need for drugs that can effectively treat and prevent communicating hydrocephalus, but unfortunately there is still a lack of effective drugs. [0003] The applicant found through previous research that the increase of free DNA in the cerebrospinal fluid is related to t...

Claims

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Application Information

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IPC IPC(8): A61K38/46A61P25/00A61P7/10
CPCA61K38/465A61P25/00A61P7/10C12Y301/21001Y02A50/30
Inventor 冯州陈志郭沛雯梁亮曾宗伟郝小可
Owner THE FIRST AFFILIATED HOSPITAL OF ARMY MEDICAL UNIV
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