Collagen gel as well as preparation method and application thereof

A collagen and gel technology, applied in the field of medical cosmetology, can solve problems such as being easily degraded by the body and cannot fundamentally solve the problem of wrinkles, and achieve the effects of promoting healing and growth, eliminating wrinkles and improving histocompatibility.

Inactive Publication Date: 2021-11-23
白晋
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

Removing wrinkles through autologous collagen injection can significantly reduce allergic reactions, but simple collagen injection is easily degraded by the body and cannot fundamentally solve the problem of wrinkles. Therefore, the existing medical collagen injection fillers are improved. It is of great significance to improve the effect of removing wrinkles

Method used

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preparation example Construction

[0029] The embodiment of the present invention provides a preparation method of autologous collagen gel with hyaluronic acid, comprising the following steps:

[0030] Step 1: Obtain the autologous fibroblast tissue, wash it with normal saline at room temperature, cut the autologous fibroblast tissue into small pieces, add phosphate buffered saline solution to it and mix, centrifuge at room temperature to remove the lower layer liquid, rinse with phosphate buffered saline solution Centrifuge again, the centrifugal speed is 1000rpm-2500rpm, the centrifugation time is 2min-5min, remove the supernatant, add collagenase, place it in a constant temperature shaking box at 37°C for 30min-60min, centrifuge again to remove the supernatant, and then add fetal bovine The DMEM culture fluid of serum, the content of fetal bovine serum in DMEM culture fluid is 15%, centrifuges to remove supernatant after filtering with cell sieve, adds in the DMEM culture fluid containing fetal bovine serum a...

Embodiment 1

[0041] Autologous collagen gel with hyaluronic acid was prepared using the following steps:

[0042] Step 1: Obtain the autologous fibroblast tissue, wash it with normal saline at room temperature, cut the autologous fibroblast tissue into small pieces, add phosphate buffered saline solution to it and mix, centrifuge at room temperature to remove the lower layer liquid, rinse with phosphate buffered saline solution Centrifuge again, the centrifugal speed is 1000rpm, the centrifugation time is 5min, remove the supernatant, add collagenase, place in a constant temperature shaking box at 37°C for 60min, centrifuge again to remove the supernatant, and then add DMEM culture medium containing fetal bovine serum, The content of fetal bovine serum in the DMEM culture medium is 15%. After filtering with a cell sieve, the supernatant is removed by centrifugation, and then added to the DMEM culture medium containing fetal bovine serum. 2 Constant temperature culture;

[0043] Step 2, su...

Embodiment 2

[0048] Autologous collagen gel with hyaluronic acid was prepared using the following steps:

[0049] Step 1: Obtain the autologous fibroblast tissue, wash it with normal saline at room temperature, cut the autologous fibroblast tissue into small pieces, add phosphate buffered saline solution to it and mix, centrifuge at room temperature to remove the lower layer liquid, rinse with phosphate buffered saline solution Centrifuge again at a centrifugal speed of 1500rpm for 4min, remove the supernatant, add collagenase, place in a constant temperature shaking box at 37°C for 60min, centrifuge again to remove the supernatant, and then add DMEM culture medium containing fetal bovine serum, The content of fetal bovine serum in the DMEM culture medium is 15%. After filtering with a cell sieve, the supernatant is removed by centrifugation, and then added to the DMEM culture medium containing fetal bovine serum. 2 Constant temperature culture;

[0050] Step 2, subculture, the fibroblast...

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PUM

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Abstract

The invention relates to collagen gel as well as a preparation method and application thereof, and belongs to the technical field of medical cosmetology. The collagen gel provided by the invention comprises collagen, hyaluronic acid, a cross-linking agent and a vascular endothelial cell growth factor. The collagen generated by fibroblasts is used as a raw material, the hyaluronic acid and the cross-linking agent are mixed to prepare a gel product, and the autologous collagen gel is free of toxic and side effects, has good biocompatibility and histocompatibility, can be used as an organism tissue filler in medical cosmetology. Wherein the vascular endothelial cell growth factor can promote healing and growth of subcutaneous blood vessels after filling, promote blood circulation, moisten skin, eliminate wrinkles and prevent stains, and has a very good beautifying effect.

Description

[0001] This application is a divisional application with an application date of August 15, 2018, an application number of 201810930849.7, and an invention title of "A Method for Preparation of Autologous Collagen Gel with Hyaluronic Acid". technical field [0002] The invention relates to a preparation method of autologous collagen gel with hyaluronic acid, belonging to the technical field of medical cosmetology. Background technique [0003] Wrinkles refer to the small fine lines and wrinkles formed by the skin being affected by the external environment to form free radicals, free radicals destroy collagen and active substances in normal cell membrane tissues, and oxidize cells. There are four reasons for the occurrence of wrinkles: natural aging, gravity, ultraviolet radiation in the sun, which causes photoaging and photodamage of the skin and excessive contraction of facial expression muscles. Among them, the wrinkles produced by excessive contraction of facial expression...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): A61L27/20A61L27/22A61L27/24A61L27/50A61L27/52C07K14/78C12P21/02
CPCA61L27/20A61L27/227A61L27/24A61L27/50A61L2430/34A61L2400/06A61L27/52C07K14/78C12P21/02C08L5/08C08L89/00
Inventor 白晋
Owner 白晋
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