Paraffin section of camellia oleifera flower bud as well as preparation method and application of paraffin section

A technology of paraffin sectioning and Camellia oleifera, applied in the preparation of test samples, etc., can solve the problems of material fragmentation, differences in processing methods, and affecting the quality of sections, so as to prevent changes in shape and structure, maintain integrity, and have good transparency Effect

Pending Publication Date: 2021-11-26
HAINAN UNIVERSITY
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

The basic steps of paraffin section include sampling and fixing, softening, dehydration, transparency, paraffin dipping, embedding, waxing, slicing, spreading, drying, dewaxing, rehydration, staining, sealing and observation, etc. It is more complicated, the cycle is long, and each step is very important. Plants with different tissue structures and tissues of different parts of plants require different processing methods.
Problems with the processing method or operation will affect the quality of the slices, such as broken materials, incomplete slices, discontinuous wax bands, and cavities.
[0005] Camellia oleifera flower buds have difficulties in dehydration, wax dipping, and slicing due to their dense tissue structure. Improper handling will easily cause problems such as incomplete slices and unclear tissue structure, which is not conducive to observation
At present, there are few studies on the paraffin section technology using Camellia oleifera flower buds as materials, and the sectioning effect is not ideal, and there are no reports on Camellia oleifera flower bud slices with a more complete and clear structure.

Method used

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  • Paraffin section of camellia oleifera flower bud as well as preparation method and application of paraffin section
  • Paraffin section of camellia oleifera flower bud as well as preparation method and application of paraffin section
  • Paraffin section of camellia oleifera flower bud as well as preparation method and application of paraffin section

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Experimental program
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Embodiment 1

[0071] The specific steps of this embodiment are as follows:

[0072] (1) Segmentation and fixation: Use scissors or blades to collect flower buds together with flower stalks, take them back to the laboratory immediately for selective segmentation, peel off the sepals with tweezers, and cut off 1 / 3-1 / 3 of the flower buds longitudinally with a new clean blade 5 volumes of tissue, then quickly put it into a 50ml centrifuge tube filled with fixative solution for soaking, place 3-5 flower buds in each tube, close the lid tightly, mark the date, processing, sample, collector and other information, materials and fixative solution The volume ratio of the oleifera must be greater than 1:20g / ml, and vacuum treatment is carried out every day, and the rest of the time is stored at 4°C. After fixing for 7 days, the fixed camellia flower buds are obtained; the length of the camellia oleifera flower buds is 1.05cm; the fixative is to be analyzed Pure formaldehyde solution, glacial acetic ac...

Embodiment 2

[0090] Compared with Example 1, the difference is that the length of the Camellia oleifera flower buds collected is 1.53cm, and the paraffin section effect of the Camellia oleifera flower buds in this embodiment is shown in figure 2 ,in figure 2 a is a section of Camellia oleifera flower buds under a microscope at 0.63 times, figure 2 B is a partially enlarged view (10 times) of the Camellia oleifera flower bud section of embodiment 2, figure 2 C is the magnified view (40 times) of anther tissue of embodiment 2 Camellia oleifera flower bud section, from figure 2 It can be seen that the slicing effect of Example 2 is similar to that of Example 1, and will not be described in detail, which shows that the method provided by the present invention is also applicable to larger flower buds and has a wide range of applications.

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Abstract

The invention discloses a paraffin section of a camellia oleifera flower bud as well as a preparation method and application of the paraffin section. The method comprises the operation steps of material cutting and fixing, primary dehydration, primary transparent treatment, wax soaking, embedding, wax trimming, slicing, section bonding, section unfolding, section drying treatment and the like. The paraffin section prepared by the method disclosed by the invention is high in quality, clear and complete in tissue structure, obvious in effect, high in operability, high in repeatability and high in practicability; and the method provided by the invention can be used for carrying out paraffin slicing on camellia oleifera flower buds in different development stages, so the whole camellia oleifera flower development process is observed.

Description

technical field [0001] The invention belongs to the technical field of paraffin sections, and in particular relates to a paraffin section and its preparation method and application, in particular to a paraffin section of Camellia oleifera flower buds and its preparation method and application. Background technique [0002] Camellia oleifera (Camellia oleifera Abel.) is a small tree of the genus Camellia (Theaceae) in the family Theaceae. It is native to China and is known as the world's four major woody oil plants together with oil palm, coconut, and olive. Camellia oleifera seed oil is rich in specific physiologically active substances such as unsaturated fatty acids, vitamin E, and camellia glycosides. It has extremely high edible and medical care values, and is deeply loved by consumers. [0003] Plant flowering is an important basis for plants to form economic yields. Camellia oleifera is monoecious, because of its extremely low self-fertilization rate, severe flower and...

Claims

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Application Information

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IPC IPC(8): G01N1/28G01N1/30
CPCG01N1/28G01N1/30
Inventor 陈金辉孔令珊王健
Owner HAINAN UNIVERSITY
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