Immune cell receptor transcriptome diversity detection multi-primer intelligent selection method based on base sequence matching grouping

Abstract

The invention discloses an immune cell receptor transcriptome diversity detection multi-primer intelligent selection method based on base sequence matching grouping, which comprises the following steps: segmenting m FR3 sequences into sub-strings with different lengths, de-weighting the sub-strings, further screening out a sub-string set meeting requirements according to sub-string screening conditions (primer length, annealing temperature and CG content), and selecting the sub-string set according to the sub-string screening conditions (primer length, annealing temperature and CG content) to obtain the immune cell receptor transcriptome diversity detection multi-primer intelligent selection method. Through weighted selection of substrings and grouping of original base sequences, the public continuous substrings of each group are as long as possible under the condition that the number of groups is as small as possible, and finally, the optimal degenerate primer set is screened out, so that the primer design efficiency is greatly improved, and the experiment difficulty and cost are effectively reduced.

Description

technical field [0001] The invention relates to the technical field of automatic screening of multiple primers for immune repertoires, in particular to an intelligent selection method for multiple primers based on character string matching and weighted selection of substrings and grouping. Background technique [0002] The immune repertoire (Immune Repertoire, IR) refers to the sum of all functionally diverse B cells and T cells in the individual's circulatory system at any given time. The diversity of the immune system is crucial to the health of the body. The richer the diversity, the healthier the body's immune system, and the stronger the ability to recognize and clear antigens. The body's acquired immunity can recognize countless antigenic molecules and respond accordingly. Its molecular basis is that T cell surface receptors and B cell surface receptors (TCR and BCR) specifically recognize antigen segments and activate immune responses. The T cell repertoire and B ce...

AI Technical Summary

Problems solved by technology

However, a disadvantage of multiplex PCR is that primer dimers will be formed between different primers, and the more pairs of primers, the more serious the dimer phenomenon, which greatly reduces the amplification efficiency of the primers.

Moreover, the greater the number of primer pairs, the higher the cost and difficulty of the experiment, so how to amplify the largest library with the least number of primers becomes a challenge

Images