Instant-preparation instant-use semen diluent for improving fertilization rate of Yellow River catfish and preparation method thereof

Abstract

The invention provides an instant-preparation instant-use semen diluent for improving fertilization rate of Yellow River catfish and a preparation method thereof, belongs to the field of fish semen diluents, and mainly solves the problem that the artificial breeding scale is affected due to insufficient semen during large-scale artificial breeding of the Yellow River catfish. According to the instant-preparation instant-use semen diluent for improving the fertilization rate of the Yellow River catfish, the semen diluent comprises male catfish seminal fluid and water, the male catfish seminal fluid and the water are mixed and activated according to the proportion of 1: 1, the activated seminal fluid and a glucose solution with the concentration of 0.6%-0.8% are diluted according to the proportion of 1: 10, and the glucose solution is a glucose solution with the concentration of 0.8%. The preparation method of the semen diluent comprises the following steps: step 1, preparing glucose with the concentration of 0.8% as an isotonic solution; step 2, collecting seminal fluid of male Yellow River catfish; step 3, mixing and activating the seminal fluid collected in the step 2 and water in the Yellow River according to the ratio of 1: 1; and step 4, diluting the semen activated in the step 3 with a 0.8% glucose solution in the ratio of 1: 10, and preserving.

Description

technical field [0001] The invention belongs to the field of fish semen diluent, in particular to a ready-to-use semen diluent for improving the fertilization rate of Yellow River catfish and a preparation method thereof. Background technique [0002] Yellow River catfish (Silurus Lanzhouensis), also known as Lanzhou catfish, also known as catfish and soil catfish, belongs to Siluriformes and Siluridae, and is an important local famous and high-quality economic fish in the middle and upper reaches of the Yellow River. The Gansu section of the Yellow River is the origin and main habitat of the Yellow River catfish. Yellow River catfish has good ecological habits such as fast growth, strong adaptability, low temperature resistance, and low oxygen resistance. The meat is tender and rich in various nutrients, and has a delicate taste, less spines and more meat. , enjoys the reputation of "Yellow River Live Ginseng", is widely favored by people, and presents a good utilization p...

AI Technical Summary

Problems solved by technology

[0004] The purpose of the present invention is to provide a ready-to-use semen dilution for improving the fertilization rate of Yellow River catfish and its preparation method, so as to solve the problem that the scale of artificial breeding is often affected by insufficient semen in large-scale artificial breeding of Yellow River catfish

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