Grass carp scale high-activity antifreeze polypeptide and preparation method thereof

Abstract

The invention provides a grass carp scale high-activity antifreeze polypeptide and a preparation method thereof, fish scales are taken as a raw material, the high-activity antifreeze polypeptide is obtained through pretreatment, papain enzymolysis and separation and purification, detection and analysis show that the molecular weight of the antifreeze polypeptide is 1107.54-1554.72 Da, and three main polypeptide amino acid sequences of the antifreeze polypeptide are respectively VGPAGPSGPSGPQ, RGSPGERGESGPAGPSG and VGPAGPSGPSGPQG. Fish scale waste resources are fully utilized, waste is turned into wealth, the low-cost and high-activity natural anti-freezing polypeptide is prepared, the defects and defects of common anti-freezing agents and anti-freezing proteins are overcome, and the grass carp scale high-activity antifreeze polypeptide has the advantages of being wide in source, good in safety, low in cost, good in heat stability and the like, is beneficial to industrial popularization and application of the grass carp scale high-activity antifreeze polypeptide and has good application prospects. The grass carp scale high-activity antifreeze polypeptide and the preparation method thereof provide technical support and theoretical basis for preparing the novel antifreeze polypeptide and exploring the application of the antifreeze polypeptide in the aspects of food, medicine and the like.

Description

technical field [0001] The invention belongs to the field of biotechnology, and in particular relates to a grass carp fish scale high-activity antifreeze polypeptide and a preparation method thereof. Background technique [0002] Grass carp plays an important role in the development of my country's fishery. The statistical results of the "China Fishery Statistical Yearbook" show that in 2018, the output of freshwater fish farming in my country reached 29.5984 million tons, of which grass carp produced the largest amount of 5.5043 million tons. It plays an irreplaceable role in the fields of agriculture, farmers' employment, and protection of the ecological environment. In recent years, various grass carp processed products such as prepared food, snack food, surimi products, etc. have been rising year after year. However, the increased processing of grass carp also results in the production of a large number of by-products such as fish scales. According to incomplete statisti...

AI Technical Summary

Problems solved by technology

[0015] 1. Disadvantages of the source of mammalian skin: In recent years, with the outbreak of swine fever, foot-and-mouth disease and other diseases, there are hidden dangers in the safety of antifreeze polypeptides prepared from mammalian animals

[0016] 2. The literature reports that the antifreeze activity of the antifreeze polypeptide prepared from fish skin collagen has a heat hysteresis activity of 0.63°C, and the antifreeze activity is not high enough

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